SiRNA-mediated flotillin-2 (Flot2) downregulation inhibits cell proliferation, migration, and invasion in gastric carcinoma cells

Oncology Research

Volumn 21, Issue 5, 2013, Pages 271-279

  Cao, Ke ^a   Xie, Dingfang ^a   Cao, Peiguo ^a   Zou, Qiong ^a   Lu, Can ^a   Xiao, Sheng ^a   Zhou, Jianda ^a   Peng, Xiaowei ^b  

^a CENTRAL SOUTH UNIVERSITY   (China)

^b Central South University   (China)

Author keywords

Flotillin2 (Flot2); Gastric carcinoma; Invasion; Migration; Prognosis; Proliferation

Indexed keywords

FLOTILLIN 2; SMALL INTERFERING RNA; FLOTILLINS; MEMBRANE PROTEIN;

ADULT; ARTICLE; CANCER INHIBITION; CANCER PROGNOSIS; CANCER STAGING; CANCER TISSUE; CARCINOMA CELL; CELL INVASION; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; FEMALE; FOLLOW UP; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; STOMACH CARCINOMA; SURVIVAL TIME; TUMOR VOLUME; UPREGULATION; AGED; BIOSYNTHESIS; CELL GROWTH; CELL MOTION; GENETIC TRANSFECTION; GENETICS; IMMUNOHISTOCHEMISTRY; METABOLISM; MIDDLE AGED; PATHOLOGY; PHYSIOLOGY; PROGNOSIS; STOMACH NEOPLASMS; TISSUE MICROARRAY; TUMOR INVASION; VERY ELDERLY;

ADULT; AGED; AGED, 80 AND OVER; CELL GROWTH PROCESSES; CELL MOVEMENT; DOWN-REGULATION; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MEMBRANE PROTEINS; MIDDLE AGED; NEOPLASM INVASIVENESS; PROGNOSIS; RNA, SMALL INTERFERING; STOMACH NEOPLASMS; TISSUE ARRAY ANALYSIS; TRANSFECTION;

EID: 84922055989     PISSN: 09650407     EISSN: 15553906     Source Type: Journal    
DOI: 10.3727/096504014X13946737557031     Document Type: Article

References (21)

1. Cheng, Y. X.; Hu, M.; Chen, L.; Huang, J. L.; Xia, L. B.; Li, B. S.; Zhou, L. M.; Hong, L. The mechanism of lipid raft mediating chemoresistance of cervical cancer. Saudi Med. J. 33(5):508-514. 2012.
2. Sonnino, S.; Prinetti, A. Membrane domains and the "lipid raft" concept. Curr. Med. Chem. 20(1):4-21. 2013.
3. George, K. S.; Wu, S. Lipid raft: A floating island of death or survival. Toxicol. Appl. Pharmacol. 259(3):311-319. 2012.
4. Doherty, S. D.; Prieto, V. G.; George, S.; Hazarika, P.; Duvic, M. High flotillin-2 expression is associated with lymph node metastasis and Breslow depth in melanoma. Melanoma Res. 16(5):461-463. 2006.
5. Sasaki, Y.; Oshima, Y.; Koyama, R.; Maruyama, R.; Akashi, H.; Mita, H.; Toyota, M.; Shinomura, Y.; Imai, K.; Tokino, T. Identification of flotillin-2, a major protein on lipid rafts, as a novel target of p53 family members. Mol. Cancer Res. 6(3):395-406. 2008.
6. Zhu, Z.; Wang, J.; Sun, Z.; Sun, X.; Wang, Z.; Xu, H. Flotillin2 expression correlates with HER2 levels and poor prognosis in gastric cancer. PLoS One 8(5):e62365. 2013.
7. Gong, H.; Song, L.; Lin, C.; Liu, A.; Lin, X.; Wu, J.; Li, M.; Li, J. Downregulation of miR-138 sustains NF-kappaB activation and promotes lipid raft formation in esophageal squamous cell carcinoma. Clin. Cancer Res. 19(5):1083-1093. 2013.
8. Ji, H.; Greening, D. W.; Barnes, T. W.; Lim, J. W.; Tauro, B. J.; Rai, A.; Xu, R.; Adda, C.; Mathivanan, S.; Zhao, W.; Xue, Y.; Xu, T.; Zhu, H. J.; Simpson, R. J. Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components. Proteomics 13(10-11):1672-1686. 2013.
9. Salzer, U.; Prohaska, R. Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts. Blood 97(4):1141-1143. 2001.
10. Solomon, S.; Masilamani, M.; Rajendran, L.; Bastmeyer, M.; Stuermer, C. A.; Illges, H. The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs. Immunobiology 205(1):108-119. 2002.
11. Neumann-Giesen, C.; Falkenbach, B.; Beicht, P.; Claasen, S.; Luers, G.; Stuermer, C. A.; Herzog, V.; Tikkanen, R. Membrane and raft association of reggie-1/flotillin-2: Role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression. Biochem. J. 378(Pt. 2):509-518. 2004.
12. Li, Y.; Martin, B. R.; Cravatt, B. F.; Hofmann, S. L. DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells. J. Biol. Chem. 287(1):523-530. 2012.
13. Walton, J. R.; Frey, H. A.; Vandre, D. D.; Kwiek, J. J.; Ishikawa, T.; Takizawa, T.; Robinson, J. M.; Ackerman, W. E. Expression of flotillins in the human placenta: Potential implications for placental transcytosis. Histochem. Cell Biol. 139(3):487-500. 2013.
14. Rajendran, L.; Beckmann, J.; Magenau, A.; Boneberg, E. M.; Gaus, K.; Viola, A.; Giebel, B.; Illges, H. Flotillins are involved in the polarization of primitive and mature hematopoietic cells. PLoS One 4(12):e8290. 2009.
15. Kokubo, H.; Lemere, C. A.; Yamaguchi, H. Localization of flotillins in human brain and their accumulation with the progression of Alzheimer's disease pathology. Neurosci. Lett. 290(2):93-96. 2000.
16. Wu, Y.; Chen, X.; Wang, S.; Jiang, M.; Zheng, B.; Zhou, Q.; Bi, Y.; Zhou, Z.; Huang, X.; Sha, J. Flotillin-2 is an acrosome-related protein involved in mouse spermiogenesis. J. Biomed. Res. 26(4):278-287. 2012.
17. Babuke, T.; Ruonala, M.; Meister, M.; Amaddii, M.; Genzler, C.; Esposito, A.; Tikkanen, R. Hetero-oligomerization of reggie-1/flotillin-2 and reggie-2/flotillin-1 is required for their endocytosis. Cell Signal. 21(8):1287-1297. 2009.
18. Hazarika, P.; McCarty, M. F.; Prieto, V. G.; George, S.; Babu, D.; Koul, D.; Bar-Eli, M.; Duvic, M. Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1. Cancer Res. 64(20):7361-7369. 2004.
19. Berger, T.; Ueda, T.; Arpaia, E.; Chio, I. I.; Shirdel, E. A.; Jurisica, I.; Hamada, K.; You-Ten, A.; Haight, J.; Wakeham, A.; Cheung, C. C.; Mak, T. W. Flotillin-2 deficiency leads to reduced lung metastases in a mouse breast cancer model. Oncogene 32:4989-4994; 2013.
20. Yang, X. Y.; Ren, C. P.; Wang, L.; Li, H.; Jiang, C. J.; Zhang, H. B.; Zhao, M.; Yao, K. T. Identification of differentially expressed genes in metastatic and non-metastatic nasopharyngeal carcinoma cells by suppression subtractive hybridization. Cell Oncol. 27(4):215-223. 2005.
21. Kim, J.; Adam, R. M.; Solomon, K. R.; Freeman, M. R. Involvement of cholesterol-rich lipid rafts in interleukin-6-induced neuroendocrine differentiation of LNCaP prostate cancer cells. Endocrinology 145(2):613-619. 2004.