Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by orbitrap mass spectrometry

Journal of Microbiological Methods

Volumn 110, Issue , 2015, Pages 54-60

  Tiong, Hung King ^a   Hartson, Steven ^a   Muriana, Peter M ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Adherence; Extraction; Listeria monocytogenes; Mass spectrometry; Proteomics; Surface proteins

Indexed keywords

BACTERIAL PROTEIN; BUFFER; HYPOTONIC EXTRACTION BUFFER; LITHIUM CHLORIDE; MEMBRANE PROTEIN; TRIS BUFFERED UREA; TRYPSIN AMMONIUM BICARBONATE SUCROSE BUFFER; TRYPSIN TRIS SUCROSE BUFFER; UNCLASSIFIED DRUG; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM ADHERENCE; CELL SURFACE; CELLULAR DISTRIBUTION; CONCENTRATION RESPONSE; EXTRACTION; HYDROPHILICITY; HYPOOSMOTIC STRESS; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; LISTERIA MONOCYTOGENES; MASS SPECTROMETER; MASS SPECTROMETRY; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN METABOLISM; PROTEOMICS; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY; CELL MEMBRANE; CHEMISTRY; COMPARATIVE STUDY; ISOLATION AND PURIFICATION; PROCEDURES;

BACTERIA (MICROORGANISMS); LISTERIA MONOCYTOGENES;

BACTERIAL PROTEINS; CELL MEMBRANE; CHROMATOGRAPHY, LIQUID; LISTERIA MONOCYTOGENES; MASS SPECTROMETRY; MEMBRANE PROTEINS; PROTEOMICS; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84921826517     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.01.004     Document Type: Article

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