Lysis gradient centrifugation: A flexible method for the isolation of nuclei from primary cells

Methods in Molecular Biology

Volumn 1228, Issue , 2015, Pages 15-23

  Katholnig, Karl ^a   Poglitsch, Marko ^a   Hengstschläger, Markus ^a   Weichhart, Thomas ^a  

^a NONE

Author keywords

Discontinuous density gradient; Fractionation; Mild lysis; Nuclear isolation; Single step

Indexed keywords

DETERGENT; IODIXANOL;

ANIMAL CELL; ARTICLE; CELL MANIPULATION; CELL NUCLEUS; CELL SUSPENSION; CENTRIFUGATION; CHEMOLUMINESCENCE; CONTROLLED STUDY; CYTOLYSIS; CYTOPLASM; DENSITY GRADIENT CENTRIFUGATION; FIBROBLAST; HUMAN; HUMAN CELL; JURKAT CELL LINE; LEUKEMIA CELL LINE; LYMPHOBLASTOID CELL LINE; LYSIS GRADIENT CENTRIFUGATION; MACROPHAGE; MONOCYTE; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIMARY CELL; PRIORITY JOURNAL; REPRODUCIBILITY; T LYMPHOCYTE; WESTERN BLOTTING; ANIMAL; CELL CULTURE; CELL DEATH; CELL FRACTIONATION; METABOLISM; PROCEDURES;

EUKARYOTA;

ANIMALS; BLOTTING, WESTERN; CELL DEATH; CELL FRACTIONATION; CELL NUCLEUS; CELLS, CULTURED; CENTRIFUGATION; HUMANS; MICE;

EID: 84921768513     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1680-1_2     Document Type: Article

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