Rapid isolation of nuclei from living immune cells by a single centrifugation through a multifunctional lysis gradient

Journal of Immunological Methods

Volumn 373, Issue 1-2, 2011, Pages 167-173

  Poglitsch, Marko ^a   Katholnig, Karl ^a   Säemann, Marcus D ^a   Weichhart, Thomas ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Density gradient; Discontinuous; Fractionation; Fragile; Nucleus

Indexed keywords

IODIXANOL;

ARTICLE; CELL ISOLATION; CELL NUCLEUS; CELL TYPE; CENTRIFUGATION; CYTOLYSIS; CYTOPLASM; DENSITY GRADIENT; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; ISOLATION PROCEDURE; MONOCYTE; PRIORITY JOURNAL; REPRODUCIBILITY;

BLOTTING, WESTERN; CELL FRACTIONATION; CELL LINE, TUMOR; CELL NUCLEUS; CELLS, CULTURED; CENTRIFUGATION, DENSITY GRADIENT; CYTOPLASM; HUMANS; JURKAT CELLS; LIPOPOLYSACCHARIDES; MACROPHAGES; MICROSCOPY, FLUORESCENCE; MONOCYTES; REPRODUCIBILITY OF RESULTS; SUBCELLULAR FRACTIONS; T-LYMPHOCYTES; TIME FACTORS; TRANSCRIPTION FACTORS; TRIIODOBENZOIC ACIDS;

EID: 80054838531     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.08.012     Document Type: Article

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