Preparation of cytoplasmic and nuclear RNA from tissue culture cells

Cold Spring Harbor Protocols

Volumn 5, Issue 6, 2010, Pages

  Rio, Donald C ^a   Ares Jr, Manuel ^a   Hannon, Gregory J ^a   Nilsen, Timothy W ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ARTICLE; CELL ADHESION; CELL DISRUPTION; CELL NUCLEUS; CENTRIFUGATION; CULTURE MEDIUM; CYTOPLASM; RNA EXTRACTION; RNA ISOLATION; RNA PURIFICATION; TISSUE CULTURE CELL;

EID: 77956293990     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5441     Document Type: Article

References (3)

1. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010a. Purification of RNA by SDS solubilization and phenol extraction. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5438.
2. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010b. Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5439.
3. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010c. Removal of ribosomal subunits (and rRNA) from cytoplasmic extracts before solubilization with SDS and deproteinization. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5442.