Using Caged Ligands to Study Intracrine Endothelin Signaling in Intact Cardiac Myocytes

Methods in Molecular Biology

Volumn 1234, Issue , 2015, Pages 31-41

  Merlen, Clémence ^a   Villeneuve, Louis R ^a   Allen, Bruce G ^a  

^a NONE

Author keywords

Caged ligands; Confocal microscopy; GPCRs; Immunocytofl uorescence; Intracrine signaling; Live cell imaging; Transcription

Indexed keywords

CALCIUM ION; ENDOTHELIN; LIGAND;

ADULT; ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CALCIUM CELL LEVEL; CELL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EXPOSURE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HEART MUSCLE CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE MICROSCOPY; INTRACELLULAR SIGNALING; NONHUMAN; PHOTOLYSIS; PRIORITY JOURNAL; RADIATION EXPOSURE; RAT; SECOND MESSENGER; ULTRAVIOLET IRRADIATION; ANIMAL; FLUORESCENCE MICROSCOPY; METABOLISM; MOLECULAR IMAGING; PROCEDURES;

ANIMALS; ENDOTHELINS; LIGANDS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; MYOCYTES, CARDIAC; RATS;

EID: 84921738637     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1755-6_4     Document Type: Article

References (12)

1. Vaniotis G, Allen BG, Hébert TE (2011) Nuclear GPCRs in cardiomyocytes: an insider's view of β-adrenergic receptor signaling. Am J Physiol Heart Circ Physiol 301:H1754–H1764
2. Tadevosyan A, Vaniotis G, Allen BG et al (2012) G protein-coupled receptor signalling in the cardiac nuclear membrane: evidence and possible roles in physiological and pathophysiological function. J Physiol 590:1313–1330
3. McCray JA, Trentham DR (1989) Properties and uses of photoreactive caged compounds. Annu Rev Biophys Biophys Chem 18:239–270
4. Adams SR, Tsien RY (1993) Controlling cell chemistry with caged compounds. Annu Rev Plant Physiol Plant Mol Biol 55:755–784
5. Marriott G, Walker JW (1999) Caged peptides and proteins: new probes to study polypeptide function in complex biological systems. Trends Plant Sci 4:330–334
6. Merlen C, Farhat N, Luo X et al (2013) Intracrine endothelin signaling evokes IP3- dependent increases in nucleoplasmic Ca in adult cardiac myocytes. J Mol Cell Cardiol 62:189–202
7. Vaniotis G, Glazkova I, Merlen C et al (2013) Regulation of cardiac nitric oxide signalling by nuclear β-adrenergic and endothelin receptors. J Mol Cell Cardiol 62:58–68
8. Bourgault S, Létourneau M, Fournier A (2007) Development of photolabile caged analogs of endothelin-1. Peptides 28:1074–1082
9. Rodrigues B, Severson DL (1997) Preparation of cardiomyocytes. In: McNeill JH (ed) Biochemical techniques in the heart. CRC, New York, NY, pp 101–115
10. Chevalier D, Allen BG (2000) Two distinct forms of MAPKAP kinase-2 in adult cardiac ventricular myocytes. Biochemistry 39:6145–6156
11. Ryall KA, Saucerman JJ (2012) Automated imaging reveals a concentration dependent delay in reversibility of cardiac myocyte hypertrophy. J Mol Cell Cardiol 53:282–290
12. Dingar D, Merlen C, Grandy S et al (2010) Effect of pressure overload-induced hypertrophy on the expression and localization of p38 MAP kinase isoforms in the mouse heart. Cell Signal 22:1634–1644