Immunoassay-based serum hepcidin reference range measurements in healthy children: Differences among age groups

Journal of Clinical Laboratory Analysis

Volumn 29, Issue 1, 2015, Pages 10-14

  Sdogou, Triantafyllia ^a   Tsentidis, Charalampos ^a   Gourgiotis, Dimitrios ^a   Marmarinos, Antonios ^a   Gkourogianni, Alexandra ^a   Papassotiriou, Ioannis ^a   Anastasiou, Theodora ^a   Kossiva, Lydia ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

ELISA; Ferritin; Hepcidin; Iron metabolism; Pediatrics

Indexed keywords

FERRITIN; HEPCIDIN;

ARTICLE; BLOOD LEVEL; CHILD; CLINICAL EVALUATION; CONTROLLED STUDY; ELISA KIT; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FERRITIN BLOOD LEVEL; GROUPS BY AGE; GROWTH RATE; HUMAN; HUMAN EXPERIMENT; IMMUNOASSAY; IRON METABOLISM; MALE; NORMAL HUMAN; NORMAL VALUE; REFERENCE VALUE; REPRODUCIBILITY; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; AGE; AGING; BLOOD; NONPARAMETRIC TEST; PRESCHOOL CHILD;

AGE FACTORS; AGING; CHILD; CHILD, PRESCHOOL; FEMALE; HEPCIDINS; HUMANS; IMMUNOASSAY; MALE; REFERENCE VALUES; STATISTICS, NONPARAMETRIC;

EID: 84921064219     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.21719     Document Type: Article

References (20)

1. Nguyen NB, Callaghan KD, Ghio AJ, Haile DJ, Yang F. Hepcidin expression and iron transport in alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 2006;291:L417-L425.
2. Young MF, Glahn RP, Ariza-Nieto M, et al. Serum hepcidin is significantly associated with iron absorption from food and supplemental sources in healthy young women. Am J Clin Nutr 2009;89:533-538.
3. Delaby C, Pilard N, Goncalves AS, Beaumont C, Canonne-Hergaux F. The presence of the iron exporter ferroportin at the plasma membrane of macrophages is enchanced by iron loading and downregulated by hepcidin. Blood 2005;106:3979-3984.
4. Ramey G, Deschemin JC, Burel B, Canonne-Hergaux G, Nicolas G, Vaulont S. Hepcidin targets ferroportin for degradation in hepatocytes. Haematologica 2010;95:501-504.
5. Nemeth E, Tuttle MS, Powelson J, et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internaliztion. Science 2004;306:2090-2093.
6. Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood 2003;102:783-788.
7. Kemna EH, Tjalsma H, Willems HL, Swinkels DW. Hepcidin: From discovery to differential diagnosis. Haematologica 2008;93:90-97.
8. Kroot JC, Tjalsma H, Fleming RE, Swinkels DW. Hepcidin in human iron disorders: Diagnostic impications. Clin Chem 2011;57:12:1650-1669.
9. Grebechtchikov N, Geurts-Moespot AJ, Droot JJ, et al. High-sensitive radioimmunoassay for human serum hepcidin. Br J Haematol 2009;146(3):317-325.
10. Butterfield AM, Luan P, Witcher DR, et al. A dual-monoclonal sandwich ELISA specific for hepcidin-25. Clin Chem 2010;56:1725-1732.
11. Kemna EH, Tjalsma H, Podust VN, Swinkels DW. Mass spectometry-based hepcidin measurements in serum and urine: Analytical aspects and clinical implications. Clin Chem 2007;53:620-628.
12. Kroot JJ, Hendriks JC, Laarakkers CM, et al. (Pre) analytical impression, between-subject variability and daily variations in serum and urine hepcidin: Implications for clinical studies. Anal Biochem 2009;389:124-129.
13. Ganz T, Olbina G, Girelli D, Nemeth E, Westerman M. Immunoassay for human serum hepcidin. Blood 2008;112:4292-4297.
14. Galesloot TE, Vermeulen SH, Geurts-Moespot AJ, et al. Serum hepcidin: Reference ranges and biochemical correlates in the general population. Blood 2011;117:218-225.
15. Del Giudice EM, Santoro N, Amato A, et al. Hepcidin in obese children as a potential mediator of the association between obesity and iron deficiency. J Clin Encocrinol Metab 2009;94:5102-5107.
16. Cangemi G, Pistorio A, Miano M, et al. Diagnostic potential of hepcidin testing in pediatrics. Eur J Hematol 2013;90: 323-330.
17. Aeberli I, Hurrell RF, Zimmermann MB. Overweight children have higher circulating hepcidin concentrations and lower iron status but have dietary iron intakes and bioavailability comparable with normal weight children. Int J Obes (Lond) 2009;33:1111-1117.
18. de Mast Q, Syafruddin D, Keijmel S, et al. Increased serum hepcidin and alterations in blood iron parameters associated with asymtomatic P. falciparum and P. vivax malaria. Haematologica 2010;95:1068-1074.
19. Cole TJ, Bellizzi MC, Flegal KM, Dietz WH. Establishing a standard definition for child overweight and obesity worldwide:international survey. BMJ 2000, 320, 1-6.
20. Pasricha SR, McQuilten Z, Westerman M, et al. Serum hepcidin as a diagnostic test for iron deficiency in premenopausal female blood donors. Haematologica 2011;96:1099-1105.