Rapid and quantitative detection of Fusarium oxysporum f. sp. cubense race 4 in soil by real-time fluorescence loop-mediated isothermal amplification

Journal of Applied Microbiology

Volumn 117, Issue 6, 2014, Pages 1740-1749

  Peng, Jun ^a   Zhang, He ^a   Chen, Fengping ^b   Zhang, Xin ^a   Xie, Yixian ^a   Hou, Xianwen ^a   Li, Guangyi ^a   Pu, Jinji ^a  

^a INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

^b FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY   (China)

Author keywords

Detection; Fusarium oxysporum f. sp. cubense (Foc) race 4; Loop mediated isothermal amplification; Real time fluorescence loop mediated isothermal amplification; Soil,subtropical race 4 (STR4),tropical race 4 (TR4)

Indexed keywords

FUNGI; ISOTHERMS; POLYMERASE CHAIN REACTION; SOILS;

CUBENSE (FOC) RACE 4; DETECTION; FUSARIUM OXYSPORUM F.; FUSARIUM OXYSPORUMS; LOOP MEDIATED ISOTHERMAL AMPLIFICATIONS; LOOP-MEDIATED ISOTHERMAL AMPLIFICATIONS; REAL- TIME; REAL-TIME FLUORESCENCE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION; SOIL,SUBTROPICAL RACE 4 ,TROPICAL RACE 4; SP.;

FLUORESCENCE;

PLASMID DNA;

BIOASSAY; COMPARATIVE STUDY; FUNGUS; GENETIC MARKER; PATHOGEN; REAL TIME; SOIL BIOTA; SPORE;

ARTICLE; CONTROLLED STUDY; FUNGAL DETECTION; FUNGUS SPORE; FUSARIUM OXYSPORUM; INTERMETHOD COMPARISON; LAMP SYSTEM; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; QUANTITATIVE ANALYSIS; RANDOM AMPLIFIED POLYMORPHIC DNA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL ANALYSIS; SOIL MICROFLORA; CLASSIFICATION; EVALUATION STUDY; FLUORESCENCE; FUSARIUM; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES;

CHINA;

FUSARIUM OXYSPORUM F. CUBENSE;

FLUORESCENCE; FUSARIUM; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; REAL-TIME POLYMERASE CHAIN REACTION; SOIL MICROBIOLOGY;

EID: 84920911239     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12645     Document Type: Article

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