Decreased N6Methyladenosine in peripheral blood RNA from diabetic patients is associated with FTO expression rather than ALKBH5

Journal of Clinical Endocrinology and Metabolism

Volumn 100, Issue 1, 2015, Pages E148-E154

  Shen, Fan ^a,b   Huang, Wei ^b   Huang, Jing Tao ^a   Xiong, Jun ^b   Yang, Ying ^a   Wu, Ke ^b   Jia, Gui Fang ^c   Chen, Jinyun ^d   Feng, Yu Qi ^b   Yuan, Bi Feng ^b   Liu, Song Mei ^a  

^a ZHONGNAN HOSPITAL OF WUHAN UNIVERSITY   (China)

^b WUHAN UNIVERSITY   (China)

^c PEKING UNIVERSITY   (China)

^d UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

6 N METHYLADENOSINE; MESSENGER RNA; ADENOSINE; ALKBH5 PROTEIN, HUMAN; BIOLOGICAL MARKER; DIOXYGENASE; FTO PROTEIN, HUMAN; MEMBRANE PROTEIN; N(6)-METHYLADENOSINE; PROTEIN;

ALKBH5 GENE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CASE CONTROL STUDY; CONTROLLED STUDY; DEMETHYLATION; DNA SEQUENCE; ELECTROSPRAY MASS SPECTROMETRY; EPIGENETICS; FTO GENE; GENE; GENE EXPRESSION; GENE FREQUENCY; GENETIC ASSOCIATION; HIGH RESOLUTION MELTING ANALYSIS; HIGH RISK PATIENT; HUMAN; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; MAJOR CLINICAL STUDY; MALE; MEASUREMENT ACCURACY; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PERIPHERAL CIRCULATION; RAT; REAL TIME POLYMERASE CHAIN REACTION; RECEIVER OPERATING CHARACTERISTIC; RISK ASSESSMENT; RNA ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; TANDEM MASS SPECTROMETRY; ANALOGS AND DERIVATIVES; ANIMAL; BLOOD; FEMALE; GENETIC EPIGENESIS; GENETICS; METABOLISM;

ANIMALIA; MAMMALIA; RATTUS;

ADENOSINE; ANIMALS; BIOLOGICAL MARKERS; DIABETES MELLITUS, TYPE 2; DIOXYGENASES; EPIGENESIS, GENETIC; FEMALE; HUMANS; MALE; MEMBRANE PROTEINS; PROTEINS; RATS;

EID: 84920573898     PISSN: 0021972X     EISSN: 19457197     Source Type: Journal    
DOI: 10.1210/jc.2014-1893     Document Type: Article

References (28)

1. Xu Y, Wang L, He J, et al. Prevalence and control of diabetes in Chinese adults. JAMA. 2013;310:948-959.
2. Gilbert ER, Liu D. Epigenetics: The missing link to understanding-cell dysfunction in the pathogenesis of type 2 diabetes. Epigenetics. 2012;7:841-852.
3. He C. Grand challenge commentary: RNA epigenetics? Nat Chem Biol. 2010;6:863-865.
4. Machnicka MA, Milanowska K, Osman Oglou O, et al. MODOMICS: a database of RNA modification pathways-2013 update. Nucleic Acids Res. 2013;41:D262-D267.
5. Desrosiers R, Friderici K, Rottman F. Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells. Proc Natl Acad Sci USA. 1974;71:3971-3975.
6. Jia G, Fu Y, He C. Reversibl e RNA adenosine methylation in biological regulation. Trends Genet. 2013;29:108-115.
7. Niu Y, Zhao X, Wu YS, Li MM, Wang XJ, Yang YG. N6-methyladenosine (m6A) in RNA: an old modificatio n with a novel epigenetic function. Genomics Proteomics Bioinformatics. 2013;11: 8-17.
8. Fustin JM, Doi M, Yamaguchi Y, et al. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 2013;155:793-806.
9. Wang X, Lu Z, Gomez A, et al. N-methyladenosine-dependent regulation of messenger RNA stability. Nature. 2013;505:117-120.
10. Schwartz S, Agarwala SD, Mumbach MR, et al. High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. Cell. 2013;155:1409-1421.
11. Meyer KD, Saletore Y, Zumbo P, Elemento O, Mason CE, Jaffrey SR. Comprehensive analysis of mRNA methylation reveals enrichment in 3-UTRs and near stop codons. Cell. 2012;149:1635-1646.
12. Dominissini D, Moshitch-Moshkovitz S, Schwartz S, et al. Topology of the human and mouse m6A RNA methylomes revealed by m6Aseq. Nature. 2012;485:201-206.
13. Jia G,Fu Y Zhao X.,et al. N6-methyladenosine in nuclear RNA is a major substrate of the obesity-Associated FTO Nat Chem Biol. 2011 7 885-887.
14. Zheng G, Dahl JA, Niu Y, et al. ALKBH5 is a mammalian RNA demethylase that impactsRNAmetabolism and mouse fertility. Mol Cell. 2013;49:18-29.
15. Gerken T, Girard CA, Tung YC, et al. The obesity-Associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase. Science. 2007;318:1469-1472.
16. Dina C, Meyre D, Gallina S, et al. Variation in FTO contributes to childhood obesity and severe adult obesity. Nat Genet. 2007;39: 724-726.
17. Frayling TM, Timpson NJ, Weedon MN, et al. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity. Science. 2007;316:889-894.
18. Yang J, Loos RJ, Powell JE, et al. FTO genotype is associated with phenotypic variability of body mass index. Nature. 2012;490:267-272.
19. Fischer J, Koch L, Emmerling C, Vierkotten J, Peters T, Bruning JC, Ruther U. Inactivation of the Fto gene protects from obesity. Nature. 2009;458:894-898.
20. Church C, Moir L, McMurray F, et al. Overexpression of Fto leads to increased food intake and results in obesity. Nat Genet. 2010; 42:1086-1092.
21. DoR, Bailey SD, Desbiens K, et al. Genetic variants ofFTOinfluence adiposity, insulin sensitivity, leptin levels, and resting metabolic rate in the Quebec Family Study. Diabetes. 2008;57:1147-1150.
22. Cecil JE, Tavendale R, Watt P, Hetherington MM, Palmer CN. An obesi ty-Associated FTO gene variant and increased energy intake in children. N Engl J Med. 2008;359:2558-2566.
23. Kalnina I, Zaharenko L, Vaivade I, et al. Polymorphisms inFTOand near TMEM18 associat e with type 2 diabetes and predispose to younger age at diagnosis of diabetes. Gene. 2013;527:462-468.
24. SmemoS, Tena JJ,KimKH,et al. Obesity-Associated variants within FTO form long-range functional connections with IRX3. Nature. 2014;507:371-375.
25. Souness JE, Stouffer JE, Chagoya de Sanchez V. Effect of N6-methyladenosine on fat-cell glucose metabolism. Evidence for two modes of action. Biochem Pharmacol. 1982;31:3961-3971.
26. Chen ML, Shen F, Huang W, et al. Quantification of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA from hepatocellular carcinoma tissues by capillary hydrophilic-interaction liquid chromatography/quadrupole TOF mass spectrometry. Clin Chem. 2013;59:824-832.
27. Zheng G, Dahl JA, Niu Y, et al. Sprouts of RNA epigenetics: The discovery of mammalian RNA demethylases. RNA Biol. 2013;10: 915-918.
28. Saletore Y, Chen-Kiang S, Mason CE. NovelRNAregulatory mechanisms revealed in the epitranscriptome. RNA Biol. 2013;10:342-346.