Combinatorial mutagenesis of the voltage-sensing domain enables the optical resolution of action potentials firing at 60 Hz by a genetically encoded fluorescent sensor of membrane potential

Journal of Neuroscience

Volumn 35, Issue 1, 2015, Pages 372-385

  Piao, Hong Hua ^a   Rajakumar, Dhanarajan ^a   Kang, Bok Eum ^a   Kim, Eun Ha ^a   Baker, Bradley J ^a  

^a Korea Institute of Science and Technology   (South Korea)

Author keywords

Fluorescent protein; Omega current; Voltage imaging; Voltage sensor; Voltage sensing domain; Voltage sensing phosphatase

Indexed keywords

AMINO ACID; FLUORESCENT PROTEIN; MEMBRANE PROTEIN; PEPTIDES AND PROTEINS; UNCLASSIFIED DRUG; VOLTAGE SENSING PHOSPHATASE; FLUORESCENT DYE; ION CHANNEL; PHOTOPROTEIN;

ACTION POTENTIAL; ANIMAL TISSUE; ARTICLE; CIONA INTESTINALIS; CONTROLLED STUDY; GENE EXPRESSION; GENETIC ENCODE; GENETIC PARAMETERS; GENETIC TRANSFECTION; HIPPOCAMPUS; HUMAN; HUMAN CELL; INTRACELLULAR SIGNALING; KINETICS; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MOLECULAR IMAGING; MOUSE; MUTAGENESIS; MUTATION; NERVE CELL INHIBITION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPTICAL RESOLUTION; PATCH CLAMP TECHNIQUE; PLASMID; PROCESS OPTIMIZATION; SENSOR; SEQUENCE ALIGNMENT; SPECIES; SYNAPTIC POTENTIAL; AMINO ACID SEQUENCE; ANIMAL; C57BL MOUSE; CHANNEL GATING; HEK293 CELL LINE; MOLECULAR GENETICS; OPTOGENETICS; PHYSIOLOGY; PROCEDURES; SEQUENCE HOMOLOGY;

ACTION POTENTIALS; AMINO ACID SEQUENCE; ANIMALS; CIONA INTESTINALIS; FLUORESCENT DYES; HEK293 CELLS; HUMANS; ION CHANNEL GATING; ION CHANNELS; LUMINESCENT PROTEINS; MEMBRANE POTENTIALS; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OPTOGENETICS; SEQUENCE HOMOLOGY;

EID: 84920517970     PISSN: 02706474     EISSN: 15292401     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.3008-14.2015     Document Type: Article

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