Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos

Zygote

Volumn 24, Issue 1, 2016, Pages 48-57

  Campelo, Iana S ^a   Pereira, Alexsandra F ^a   Alcântara Neto, Agostinho S ^a   Canel, Natalia G ^b   Souza Fabjan, Joanna M G ^a   Teixeira, Dárcio I A ^a   Camargo, Luiz S A ^c   Melo, Luciana M ^a   Rádis Baptista, Gandhi ^d   Salamone, Daniel F ^b   Freitas, Vicente J F ^a  

^a STATE UNIVERSITY OF CEARÁ   (Brazil)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c EMBRAPA CERRADOS   (Brazil)

^d FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

Cattle; Developmental competence; Embryotoxicity; Preimplantation embryo; qPCR

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AQUAPORIN 3; CROTAMINE; GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 3; SNAKE VENOM;

ANIMAL; BLASTOCYST; BOVINE; CYTOLOGY; DRUG EFFECTS; FEMALE; GENE EXPRESSION REGULATION; GENETICS; IN VITRO FERTILIZATION; MALE; PHYSIOLOGY;

ANIMALS; AQUAPORIN 3; BLASTOCYST; CATTLE; CROTALID VENOMS; FEMALE; FERTILIZATION IN VITRO; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GLUCOSE TRANSPORTER TYPE 1; GLUCOSE TRANSPORTER TYPE 3; MALE; SODIUM-POTASSIUM-EXCHANGING ATPASE;

EID: 84920025602     PISSN: 09671994     EISSN: 14698730     Source Type: Journal    
DOI: 10.1017/S0967199414000707     Document Type: Article

References (30)

1. Augustin, R., Pocar, P., Navarrete-Santos, A., Wrenzycki, C., Gandolfi, F., Niemann, H., Fischer, B. (2001). Glucose transporter expression is developmentally regulated in in vitro derived bovine preimplantation embryos. Mol. Reprod. Dev. 60, 370-6.
2. Barcroft, L.C., Offenberg, H., Thomsen, P., Watson, A.J. (2003). Aquaporin proteins in murine trophectoderm mediate transepithelial water movements during cavitation. Dev. Biol. 256, 342-54.
3. Camargo, L.S., Boite, M.C., Wohlres-Viana, S., Mota, G.B., Serapiaõ, R.V., Sa, W.F., Viana, J.H., Nogueira, L.A. (2011). Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. Cryobiology 63, 256-62.
4. Dussault, A.A., Pouliot, M. (2006). Rapid and simple comparison of messenger RNA levels using real-time PCR. Biol. Proc. Online 8, 1-10.
5. Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A., Ruddle, F.H. (1980). Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA 77, 7380-4.
6. Gupta, B., Levchenko, T.S., Torchilin, V.P. (2005). Intracellular delivery of large molecules and small particles by cellpenetrating proteins and peptides. Adv. Drug Deliv. Rev. 57, 637-51.
7. Gupta, S.K., Bhandari, B., Shrestha, A., Biswal, B.K., Palaniappan, C., Malhotra, S.S., Gupta, N. (2012). Mammalian zona pellucida glycoproteins: structure and function during fertilization. Cell Tissue Res. 349, 665-78.
8. Hammer, R.E., Pursel, V.G., Rexroad, Jr C.E., Wall, R.J., Bolt, D.J., Ebert, K.M., Palmiter, R.D., Brinster, R.L. (1985). Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315, 680-3.
9. Hayashi, M.A., Nascimento, F.D., Kerkis, A., Oliveira, V., Oliveira, E.B., Pereira, A., Rádis-Baptista, G., Nader, H.B., Yamane, T., Kerkis, I., Tersariol, I.L. (2008). Cytotoxic effects of crotamine are mediated through lysosomal membrane permeabilization. Toxicon 52, 508-17.
10. Holm, P., Booth, P.J., Schmidt, M.H., Greve, T., Callesen, H. (1999). High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 52, 683-700.
11. Houdebine, L.M. (2007). Transgenic animal models in biomedical research. Methods Mol. Biol. 360, 163-202.
12. Kerkis, A., Kerkis, I., Rádis-Baptista, G., Oliveira, E.B., Vianna-Morgante, A.M., Pereira, L.V., Yamane, T. (2004). Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus. FASEB J. 18, 1407-9.
13. Kues, W.A., Niemann, H. (2011). Advances in farm animal transgenesis. Prev. Vet. Med. 102, 146-56.
14. Kuzmany, A., Havlicek, V., Wrenzycki, C., Wilkening, S., Brem, G., Besenfelder, U. (2011). Expression of mRNA, before and after freezing, in bovine blastocysts cultured under different conditions. Theriogenology 75, 482-94.
15. Livak, K.J., Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-C T method. Methods 25, 402-8.
16. Nascimento, F.D., Hayashi, M.A., Kerkis, A., Oliveira, V., Oliveira, E.B., Rádis-Baptista, G., Nader, H.B., Yamane, T., Tersariol, I.L.S., Kerkis, I. (2007). Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. J. Biol. Chem. 282, 21349-60.
17. Nascimento, F.D., Sancey, L., Pereira, A., Rome, C., Oliveira, V., Oliveira, E.B., Nader, H.B., Yamane, T., Kerkis, I., Tersariol, I.L.S., Coll, J., Hayashi, M.A.F. (2012). The natural cell-penetrating peptide crotamine targets tumor tissue in vivo and triggers a lethal calcium-dependent pathway in cultured cells. Mol. Pharmaceutics 9, 211-21.
18. Palmiter, R.D., Brinster, R.L., Hammer, R.E., Trumbauer, M.E., Rosenfeld, M.G., Birnberg, N.C., Evans, R.M. (1982). Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300, 611-5.
19. Purcell, S.H., Moley, K.H. (2009). Glucose transporters in gametes and preimplantation embryos. Trends Endocrinol. Metab. 20, 483-9.
20. Pursel, V.G., Wall, R.J., Rexroad, Jr C.E., Hammer, R.E., Brinster, R.L. (1985). A rapid whole-mounted staining products for nuclei of mammalian eggs. Theriogenology 24, 687-91.
21. Rádis-Baptista, G., Kerkis, I. (2011). Crotamine, a small basic polypeptide myotoxin from rattlesnake venom with cell-penetrating properties. Curr. Pharm. Des. 17, 4351-61.
22. Rádis-Baptista, G., de la Torre, B.G., Andreu, D. (2008). A novel cell-penetrating peptide sequence derived by structural minimization of a snake toxin exhibits preferential nucleolar localization. J. Med. Chem. 51, 7041-4.
23. Richard, J.P., Melikov, K., Vives, E., Ramos, C., Verbeure, B., Gait, M.J., Chernomordik, L.V., Lebleu, B. (2003). Cellpenetrating peptides: a reevaluation of the mechanism of cellular uptake. J. Biol. Chem. 278, 585-90.
24. Rideout, W.M., Eggan, K., Jaenisch, R. (2001). Nuclear cloning and epigenetic reprogramming of the genome. Science 293, 1093-8.
25. Rizos, D., Clemente, M., Bermejo-Alvarez, P., Fuente, J., Lonergan, P., Gutiérrez-Adán, A. (2008). Consequences of in vitro culture conditions on embryo development and quality. Reprod. Dom. Anim. 43, 44-50.
26. Rodrigues, M., Santos, A., Torre, B.G., Rádis-Baptista, G., Andreu, D., Santos, N.C. (2012). Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes. Biochim. Biophys. Acta 1818, 2707-17.
27. Schwarze, S.R., Dowdy, S.F. (2000). In vivo protein transduction: intracellular delivery of biologically active proteins, compounds and DNA. Trends Pharmacol. Sci. 21, 45-8.
28. Tervit, H., Whittingham, D., Rowson, L. (1972). Successful culture in vitro of sheep and cattle ova. J. Reprod. Fertil. 30, 493-7.
29. Vandesompele, J., Preter, D.K., Pattynm, F., Poppe, B., Roy, N.V., Paepe, A.D., Speleman, F. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Gen. Biol. 3, 1-12.
30. Watson, A.J., Westhusin, M.E., De Sousa, P.A., Betts, D.H., Barcroft, L.C. (1999). Gene expression regulating blastocyst formation. Theriogenology 51, 117-33.