Cloning and characterization of a novel vacuolar Na+/H+ antiporter gene (VuNHX1) from drought hardy legume, cowpea for salt tolerance

Plant Cell, Tissue and Organ Culture

Volumn 120, Issue 1, 2015, Pages 19-33

  Mishra, Sagarika ^a   Alavilli, Hemasundar ^b   Lee, Byeong ha ^b   Panda, Sanjib Kumar ^c,e   Sahoo, Lingaraj ^a,d  

^a INDIAN INSTITUTE OF TECHNOLOGY GUWAHATI   (India)

^b Sogang University   (South Korea)

^c ASSAM UNIVERSITY   (India)

^d GIFU UNIVERSITY   (Japan)

^e OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Cowpea; Na+ H+ antiporter gene; RACE; Salt tolerance; Transgenic Arabidopsis

Indexed keywords

CLONE CELLS; CROPS; DROUGHT; GENES; LITHIUM COMPOUNDS; POTASSIUM COMPOUNDS; SODIUM CHLORIDE; YEAST; ION EXCHANGE;

BIOINFORMATIC ANALYSIS; COWPEA; EXPRESSION ANALYSIS; RACE; SALT TOLERANCE; SALT-SENSITIVE PHENOTYPES; TRANSGENIC ARABIDOPSIS; VACUOLAR SEQUESTRATIONS; ABIOTIC STRESS; NA +/H + ANTIPORTER; NA+/H+ ANTIPORTER GENE; SALT STRESSS; SALT TOLERANT; SOIL SALINITY;

ION EXCHANGE; DROUGHT;

ARABIDOPSIS; VIGNA UNGUICULATA;

EID: 84919838971     PISSN: 01676857     EISSN: 15735044     Source Type: Journal    
DOI: 10.1007/s11240-014-0572-7     Document Type: Article

References (72)

1. Ali R, Brett CL, Mukherjee S, Rao R (2004) Inhibition of sodium/proton exchange by a Rab-GTPase-activating protein regulates endosomal traffic in yeast. J Biol Chem 279:4498–4506
2. + antiport in Arabidopsis. Science 285:1256–1258
3. + antiporter. Plant J 36:229–239
4. + antiporter cDNA from Eucalyptus globules. Biochem Biophys Res Commun 430:535–540
5. + Antiporter gene of alfalfa enhances salinity tolerance in transgenic Arabidopsis. Acta Agron Sin 34:557–564
6. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
7. Brett CL, Donowitz M, Rao R (2005a) The evolutionary origins of eukaryotic sodium/proton exchangers. Am J Physiol Cell Physiol 288:223–239
8. + exchanger Nhx1 regulates cellular ph to control vesicle trafficking. Mol Biol Cell 16:1396–1405
9. Brini F, Gaxiola RA, Berkowitz GA, Masmoudi K (2005) Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump. Plant Physiol Biochem 43:347–354
10. +-antiport in Mesembryanthemum crsytallinum. Plant J 24:511–522
11. + antiporter gene AtNHX1 in common buckwheat (Fagopyrum esculentum). Transgenic Res 17:121–132
12. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
13. + exchangers. Biochem J 351:241–249
14. Duzdemir O, Unlukara A, Kurunc A (2009) Response of cowpea (Vigna unguiculata) to salinity and irrigation regimes. N Z J Crop Hort Sci 37:271–280
15. + exchanger gene in Oryza sativa. Biochim Biophys Acta 1446:149–155
16. + antiporter from rice. Plant Cell Physiol 45:146–159
17. Gattiker A, Gasteiger E, Bairoch A (2002) ScanPROSITE: a reference implementation of a PROSITE scanning tool. Appl Bioinformatics 1:107–108
18. Geourjon C, Deleage G (1995) SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments. Comput Appl Biosci 11:681–684
19. Gietz D, St. Jean A, Woods RA, Schiestl RH (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425
20. +-pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance. Plant Mol Biol 79:137–155
21. Hall AE (2012) Phenotyping cowpeas for adaptation to drought. Front Physiol 3:00155
22. + antiporter gene from the halophyte Atriplex gmelini. Plant Mol Biol 46:35–42
23. Hanana M, Cagnac O, Yamaguchi T, Hamdi S, Ghorbel A, Blumwald E (2007) A grape berry (Vitis vinifera L.) cation/proton antiporter is associated with berry ripening. Plant Cell Physiol 48:804–811
24. +) homeostasis and salt tolerance of plants. Environ Exp Bot 92:19–31
25. Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Bio 51:463–499
26. He CX, Yan JQ, Shen GX, Fu LH, Holaday AS, Auld D, Blumwald E, Zhang H (2005) Expression of an arabidopsis vacuolar sodium/proton antiporter gene in cotton improves photosynthetic performance under salt conditions and increases fiber yield in the field. Plant Cell Physiol 46:1848–1854
27. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetic and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
28. + antiporter AtNHX1 conferring increased salt tolerance in yeast: the endosome/prevacuolar compartment is a target for salt toxicity. J Biol Chem 284:14276–14285
29. Hofmann K, Stoffel W (1993) A database of membrane spanning proteins segments. Biol Chem 374:166
30. + antiporter gene SbNHX1 and its expression by abiotic stress. Mol Biol Rep 38:1965–1973
31. + antiporter gene of Rosa hybrida. Genes Genet Syst 80:121–128
32. +-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters. Folia Microbiol 49:519–525
33. Kronzucker HJ, Britto DT (2011) Sodium transport in plants: a critical review. New Phytol 189:54–81
34. Lee BH, Zhu JK (2010) Phenotypic analysis of Arabidopsis mutants: germination rate under salt/hormone-induced stress. Cold Spring Harb Protoc 2010:pdb-prot4969
35. Li WYF, Wong FL, Tsai SN, Phang TH et al (2006) Tonoplast-located GmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (BY)-2 cells. Plant Cell Environ 29:1122–1137
36. Lichtenthaler HK (1987) Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown. J Plant Physiol 131:101–110
37. + antiporter gene in Suada salsa under salt stress. Biol Plant 48:219–225
38. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
39. Murillo-Amador B, Troyo-Dieguez E, Garcia-Hernandez JL et al (2006) Effect of NaCl salinity in the genotypic variation of cowpea (Vigna unguiculata) during early vegetative growth. Sci Hortic 108:423–431
40. + antiporter gene from Atriplex gmelini confers salt tolerance to rice. FEBS Lett 532:279–282
41. + antiporter from Agropyron elongatum, confers salt tolerance to Arabidopsis and Festuca plants. Plant Cell Rep 26:1663–1672
42. + antiporter gene from Malus zumi. Afr J Biotechnol 8:4774–4781
43. + antiporters. FEBS Lett 471:224–228
44. + antiporter confers high level of salinity tolerance in transgenic Brassica juncea. Mol Breed 19:137–151
45. + antiporters in plants. Mol Plant 7:261–263
46. Rodriguez-Navarro A, Ramos J (1984) Dual system for potassium transport in Saccharomyces cerevisiae. J Bacteriol 159:940–945
47. + antiporter gene AtNHX1 by salt stress and abscisic acid. Plant Mol Biol 50:543–550
48. Singh BB (2005) Cowpea [Vigna unguiculata (L.) Walp. In: Singh RJ, Jauhar PP (eds) Genetic resources, chromosomal engineering and crop improvement, vol 1. CRC Press, Boca Raton, pp 117–162
49. + antiporter: impact of AtNHX1 on gene expression. Plant J 40:752–771
50. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
51. + antiporter gene TrNHX1 from Trifolium repens L. Plant Mol Biol Rep 28:102–111
52. + transport in higher plants. Ann Bot 91:503–527
53. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgin DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
54. Timko MP, Ehlers JD, Roberts PA (2007) Cowpea. In: Kole (ed) Genome mapping and molecular breeding in plants, vol 3, pulses, sugar and tuber crops. Springer, Berlin pp 49–67
55. + ion homeostasis in plants. J Biol Chem 278:22453–22459
56. + antiporter gene from Brassica napus. DNA Seq 14:351–358
57. + antiporter gene from Cucumis melo L. Afr J Biotechnol 10:1752–1759
58. + antiporter plays an important role in salt stress. Plant Cell Physiol 45:600–607
59. + antiporter gene. Plant Sci 169:65–73
60. + antiporter gene ThNHX1 from a halophytic plant Thellungiella halophila. Plant Mol Biol Rep 27:1–12
61. + antiporter in Beta vulgaris. Physiol Plant 116:206–212
62. +. Plant Sci 167:849–859
63. + exchanger and flower coloration. Plant Cell Physiol 42:451–461
64. + antiporter reveals a luminal C terminus that regulates antiporter cation selectivity. Proc Natl Acad Sci USA 100:12510–12515
65. Zhang HX, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765–768
66. Zhang HX, Hodson JN, Williams JP, Blumwald E (2001) Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci USA 98:12832–12836
67. + antiporter gene from the monocot halophyte Aeluropus littoralis. Plant Physiol Biochem 46:117–126
68. + antiporter gene, DmNHX1, confers salt tolerance when expressed transiently in Nicotiana benthamiana or stably in Arabidopsis thaliana. Plant Cell Tissue Organ Cult 110:189–200
69. + antiporter gene from Arabidopsis. J Plant Physiol 164:1377–1383
70. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71
71. Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6:441–445
72. + antiporters (ZmNHX) of maize (Zea mays L.) and their expression under salt stress. J Plant Physiol 162:55–65