Processing cellular spheroids for histological examination

Journal of Histotechnology

Volumn 37, Issue 4, 2014, Pages 138-142

  Olsen, Timothy R ^a   Mattix, Brandon ^a   Casco, Megan ^a   Herbst, Austin ^a   Williams, Colby ^a   Tarasidis, Anna ^a   Evans, Grace ^a   Jenkins, Linda ^a   McMahan, Chad L ^a   Simionescu, Dan ^a   Visconti, Richard P ^b   Alexis, Frank ^a  

^a Clemson University   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Magnetic nanoparticles; Spheroid histology; Spheroids; Tissue engineering

Indexed keywords

MAGNETIC NANOPARTICLE; ULTRASMALL SUPERPARAMAGNETIC IRON OXIDE;

ANIMAL CELL; ARTICLE; EMBEDDING; EXTRACELLULAR MATRIX; HISTOLOGY; MICROSCOPE; MULTICELLULAR SPHEROID; NONHUMAN; PROCESSING; RAT; TISSUE ENGINEERING; TUMOR SPHEROID;

EID: 84919384094     PISSN: 01478885     EISSN: 20460236     Source Type: Journal    
DOI: 10.1179/2046023614Y.0000000047     Document Type: Article

References (16)

1. Achilli TM, Meyer J, Morgan JR. Advances in the formation, use and understanding of multi-cellular spheroids. Expert Opin Biol Ther. 2012;12:1347–60.
2. Mattix B, Olsen TR, Moore T, Casco M, Simionescu D, Visconti RP, et al. Accelerated iron oxide nanoparticle degradation mediated by polyester encapsulation within cellular spheroids. Adv Funct Mater. 2014;24:800–7.
3. Mattix B, Olsen TR, Gu Y, Casco M, Herbst A, Simionescu DT, et al. Biological magnetic cellular spheroids as building blocks for tissue engineering. Acta Biomater. 2014;10:623–9.
4. Mattix BM, Olsen TR, Casco M, Reese L, Poole JT, Zhang J, et al. Janus magnetic cellular spheroids for vascular tissue engineering. Biomaterials. 2014;35:949–60.
5. Athanasiou KA, Eswaramoorthy R, Hadidi P, Hu JC. Self-organization and the self-assembling process in tissue engineering. Annu Rev Biomed Eng. 2013;15:115–36.
6. Jakab K, Norotte C, Marga F, Murphy K, Vunjak-Novakovic G, Forgacs G. Tissue engineering by self-assembly and bio-printing of living cells. Biofabrication. 2010;2.
7. Mattix B, Poole T, Casco M, Uvarov O, Vicsonti RP, Simionescu D, et al. Small diameter vascular grafts: replacement strategies, maturation techniques and challenges. J Tissue Sci Eng. 2013;4.
8. Layer PG, Robitzki A, Rothermel A, Willbold E. Of layers and spheres: the reaggregate approach in tissue engineering. Trends Neurosci. 2002;25:131–4.
9. Kelm JM, Ehler E, Nielsen LK, Schlatter S, Perriard JC, Fussenegger M. Design of artificial myocardial microtissues. Tissue Eng. 2004;10:201–14.
10. Kelm JM, Djonov V, Ittner LM, Fluri D, Born W, Hoerstrup SP, et al. Design of custom-shaped vascularized tissues using microtissue spheroids as minimal building units. Tissue Eng. 2006;12:2151–60.
11. Abu-Absi SF, Friend JR, Hansen LK, Hu WS. Structural polarity and functional bile canaliculi in rat hepatocyte spheroids. Exp Cell Res. 2002;274:56–67.
12. Riccalton-Banks L, Liew C, Bhandari R, Fry J, Shakesheff K. Long-term culture of functional liver tissue: three-dimensional coculture of primary hepatocytes and stellate cells. Tissue Eng. 2003;9:401–10.
13. Lehnert L, Trost H, Schmiegel W, Roder C, Kalthoff H. Hollow-spheres: a new model for analyses of differentiation of pancreatic duct epithelial cells. Ann NY Acad Sci. 1999;880:83–93.
14. Alajati A, Laib AM, Weber H, Boos AM, Bartol A, Ikenberg K, et al. Spheroid-based engineering of a human vasculature in mice. Nat Meth. 2008;5:439–45.
15. Olsen TR, Alexis F. Bioprocessing of tissues using cellular spheroids. J Bioprocess Biotech. 2014;4.
16. Marga F, Jakab K, Khatiwala C, Shepherd B, Dorfman S, Hubbard B, et al. Toward engineering functional organ modules by additive manufacturing. Biofabrication. 2012;4.