Resolubilization of precipitated intact membrane proteins with cold formic acid for analysis by mass spectrometry

Journal of Proteome Research

Volumn 13, Issue 12, 2014, Pages 6001-6012

  Doucette, Alan A ^a   Vieira, Douglas B ^a   Orton, Dennis J ^b   Wall, Mark J ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

acetone; chloroform; formic acid; GELFrEE; membrane protein; precipitation; top down MS

Indexed keywords

CHLOROFORM; DODECYL SULFATE SODIUM; ESCHERICHIA COLI PROTEIN; FORMIC ACID; MEMBRANE PROTEIN; METHANOL; ORGANIC SOLVENT; PROTEIN; WATER; ACETONE; FORMIC ACID DERIVATIVE; PROTEOME;

ARTICLE; FORMYLATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRECIPITATION; PROTEIN ANALYSIS; PROTEOMICS; ROOM TEMPERATURE; SUPERNATANT; ULTRASOUND; CELL MEMBRANE; CHEMISTRY; COLD; CYTOSOL; LIQUID CHROMATOGRAPHY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; REPRODUCIBILITY; SOLUBILITY;

ACETONE; CELL MEMBRANE; CHLOROFORM; CHROMATOGRAPHY, LIQUID; COLD TEMPERATURE; CYTOSOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI PROTEINS; FORMATES; FRACTIONAL PRECIPITATION; MASS SPECTROMETRY; MEMBRANE PROTEINS; METHANOL; MOLECULAR WEIGHT; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SOLUBILITY; WATER;

EID: 84915781391     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr500864a     Document Type: Article

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