Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

Applied and Environmental Microbiology

Volumn 80, Issue 21, 2014, Pages 6677-6684

  Liang, Youyun ^a   Si, Tong ^b   Ang, Ee Lui ^a   Zhao, Huimin ^a,b  

^a INSTITUTE OF CHEMICAL AND ENGINEERING SCIENCES   (Singapore)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSE; ENZYMES; ETHANOL; FERMENTATION; PHOSPHORIC ACID; SACCHARIFICATION;

CELLOBIOSE DEHYDROGENASE; CELLULOSIC MATERIAL; CELLULOSIC SUBSTRATES; CONSOLIDATED BIO-PROCESSING; ETHANOL FERMENTATION; LIGNOCELLULOSIC MATERIAL; PHOSPHORIC-ACID SWOLLEN CELLULOSE; SIMULTANEOUS SACCHARIFICATION AND FERMENTATION;

YEAST;

CELLULOSE; ENZYME ACTIVITY; ETHANOL; FERMENTATION; LIGNIN; SECRETION; SUBSTRATE; SYNERGISM; YEAST;

CELLULOSE; ETHANOL; FERMENTATION; YEASTS;

SACCHAROMYCES CEREVISIAE;

ALCOHOL; CARBON; CELLULOSE; CELLULOSOME; RECOMBINANT PROTEIN;

FERMENTATION; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

CARBON; CELLULOSE; CELLULOSOMES; ETHANOL; FERMENTATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84914169042     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02070-14     Document Type: Article

References (29)

1. Sahin Y. 2011. Environmental impacts of biofuels. Energy Education Sci. Technol. Part A Energy Sci. Res. 26:129-142.
2. Brown TR, Brown RC. 2013. A review of cellulosic biofuel commercialscale projects in the United States. Biofuels Bioprod. Biorefin. 7:235-245. http://dx.doi.org/10.1002/bbb.1387.
3. Olson DG, McBride JE, Joe Shaw A, Lynd LR. 2012. Recent progress in consolidated bioprocessing. Curr. Opin. Biotechnol. 23:396-405. http://dx.doi.org/10.1016/j.copbio.2011.11.026.
4. Hasunuma T, Kondo A. 2012. Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains. Proc. Biochem. 47:1287-1294. http://dx.doi.org/10.1016/j.procbio.2012.05.004.
5. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A. 2004. Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl. Environ. Microbiol. 70:1207-1212. http://dx.doi.org/10.1128/AEM.70.2.1207-1212.2004.
6. Den Haan R, Rose SH, Lynd LR, van Zyl WH. 2007. Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab. Eng. 9:87-94. http://dx.doi.org/10.1016/j.ymben.2006.08.005.
7. Wen F, Sun J, Zhao H. 2010. Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl. Environ. Microbiol. 76:1251-1260. http://dx.doi.org/10.1128/AEM.01687-09.
8. Yamada R, Taniguchi N, Tanaka T, Ogino C, Fukuda H, Kondo A. 2011. Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression. Biotechnol. Biofuels 4:8. http://dx.doi.org/10.1186/1754-6834-4-8.
9. Fan L-H, Zhang Z-J, Yu X-Y, Xue Y-X, Tan T-W. 2012. Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production. Proc. Natl. Acad. Sci. U. S. A. 109:13260-13265. http://dx.doi.org/10.1073/pnas.1209856109.
10. Nakatani Y, Yamada R, Ogino C, Kondo A. 2013. Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose. Microb. Cell Fact. 12:66. http://dx.doi.org/10.1186/1475-2859-12-66.
11. Zhang X-Z, Sathitsuksanoh N, Zhu Z, Percival Zhang YH. 2011. One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis. Metab. Eng. 13:364-372. http://dx.doi.org/10.1016/j.ymben.2011.04.003.
12. Horn S, Vaaje-Kolstad G, Westereng B, Eijsink VG. 2012. Novel enzymes for the degradation of cellulose. Biotechnol. Biofuels 5:45. http://dx.doi.org/10.1186/1754-6834-5-45.
13. Fushinobu S. 2014. Metalloproteins: a new face for biomass breakdown. Nat. Chem. Biol. 10:88-89. http://dx.doi.org/10.1038/nchembio.1434.
14. Podkaminer KK, Kenealy WR, Herring CD, Hogsett DA, Lynd LR. 2012. Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous saccharification and fermentation (tSSF). Biotechnol. Biofuels 5:43. http://dx.doi.org/10.1186/1754-6834-5-43.
15. Cannella D, Jørgensen H. 2014. Do new cellulolytic enzyme preparations affect the industrial strategies for high solids lignocellulosic ethanol production? Biotechnol. Bioeng. 111:59-68. http://dx.doi.org/10.1002/bit.25098.
16. Quinlan RJ, Sweeney MD, Lo Leggio L, Otten H, Poulsen J-CN, Johansen KS, Krogh KBRM, Jørgensen CI, Tovborg M, Anthonsen A, Tryfona T, Walter CP, Dupree P, Xu F, Davies GJ, Walton PH. 2011. Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc. Natl. Acad. Sci. U. S. A. 108:15079-15084. http://dx.doi.org/10.1073/pnas.1105776108.
17. Dotson W, Greenier J, Ding H. January 2006. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same. US patent 20,060,005,279.
18. Sweeney M, Vlasenko E, Abbate E. June 2011. Methods for increasing hydrolysis of cellulosic material in the presence of cellobiose dehydrogenase. US patent 20,100,159,536.
19. Shao Z, Zhao H, Zhao H. 2009. DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res. 37:e16. http://dx.doi.org/10.1093/nar/gkn991.
20. Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO. 2009. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6:343-345. http://dx.doi.org/10.1038/nmeth.1318.
21. Gietz RD, Woods RA. 2006. Yeast transformation by the LiAc/SS carrier DNA/PEG method. Methods Mol. Biol. 313:107-120. http://dx.doi.org/10.1385/1-59259-958-3:107.
22. + T-cell epitopes using yeast displaying pathogen-derived peptide library. J. Immunol. Methods 336:37-44. http://dx.doi.org/10.1016/j.jim.2008.03.008.
23. Zhang YH, Cui J, Lynd LR, Kuang LR. 2006. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure. Biomacromolecules 7:644-648. http://dx.doi.org/10.1021/bm050799c.
24. Clements JM, Catlin GH, Price MJ, Edwards RM. 1991. Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences. Gene 106:267-271. http://dx.doi.org/10.1016/0378-1119(91)90209-T.
25. Yang J, Zhang X, Yoon J, Rao K, Grate JH, Baidyaroy D, Elgart DD. February 2013. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity. US patent 20,130,052,698.
26. Sygmund C, Santner P, Krondorfer I, Peterbauer CK, Alcalde M, Nyanhongo GS, Guebitz GM, Ludwig R. 2013. Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production. Microb. Cell Fact. 12:38. http://dx.doi.org/10.1186/1475-2859-12-38.
27. Du J, Yuan Y, Si T, Lian J, Zhao H. 2012. Customized optimization of metabolic pathways by combinatorial transcriptional engineering. Nucleic Acids Res. 40:e142. http://dx.doi.org/10.1093/nar/gks549.
28. Tsai S-L, Oh J, Singh S, Chen R, Chen W. 2009. Functional assembly of minicellulosomes on the Saccharomyces cerevisiae cell surface for cellulose hydrolysis and ethanol production. Appl. Environ. Microbiol. 75:6087-6093. http://dx.doi.org/10.1128/AEM.01538-09.
29. Arfi Y, Shamshoum M, Rogachev I, Peleg Y, Bayer EA. 2014. Integration of bacterial lytic polysaccharide monooxygenases into designer cellulosomes promotes enhanced cellulose degradation. Proc. Natl. Acad. Sci. U. S. A. 111:9109-9114. http://dx.doi.org/10.1073/pnas.1404148111.