A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources

Bioresource Technology

Volumn 177, Issue , 2015, Pages 51-57

  Li, Jing ^a   Liu, Ruijie ^a   Chang, Guifang ^b   Li, Xiangyu ^a   Chang, Ming ^a   Liu, Yuanfa ^a   Jin, Qingzhe ^a   Wang, Xingguo ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Wilmar International Limited   (China)

Author keywords

Aurantiochytrium limacinum SR21; DHA; Glucose; Glycerol; Mixed carbon sources

Indexed keywords

BATCH CELL CULTURE; CARBON; FERMENTATION; GLYCEROL; PRODUCTIVITY; UNSATURATED FATTY ACIDS;

AURANTIOCHYTRIUM; CARBON SOURCE; DHA; DOCOSAHEXAENOIC ACID; ECONOMIC STRATEGY; FED-BATCH CULTURES; FED-BATCH FERMENTATION; LIPID SYNTHESIS;

GLUCOSE;

DOCOSAHEXAENOIC ACID; GLUCOSE; GLYCEROL; CARBON;

ALCOHOL; BIOTECHNOLOGY; CARBOXYLIC ACID; ECONOMIC ANALYSIS; GLUCOSE; GROWTH; MARINE TECHNOLOGY; PROTIST;

AERATION; ALGAL GROWTH; ARTICLE; AURANTIOCHYTRIUM LIMACINUM SR21; BIOSYNTHESIS; CARBON METABOLISM; CARBON SOURCE; CELL DENSITY; CELL FUNCTION; CELL GROWTH; CELL METABOLISM; FED BATCH CULTURE; FED BATCH FERMENTATION; FERMENTATION; LIPID METABOLISM; LIPID STORAGE; LIPOGENESIS; MICROALGA; NONHUMAN; NUTRIENT LIMITATION; OXYGEN SUPPLY; PENTOSE PHOSPHATE CYCLE; PRODUCTIVITY; PURIFICATION; AEROBIC METABOLISM; BATCH CELL CULTURE; BIOREACTOR; DRUG EFFECTS; METABOLISM; MICROBIOLOGY; STRAMENOPILE; TIME;

AEROBIOSIS; BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; CARBON; DOCOSAHEXAENOIC ACIDS; FERMENTATION; GLUCOSE; GLYCEROL; STRAMENOPILES; TIME FACTORS;

EID: 84913585113     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2014.11.046     Document Type: Article

References (30)

1. Abbad-Andaloussi S., Amine J., Gerard P., Petitdemange H. Effect of glucose on glycerol metabolism by Clostridium butyricum DSM 5431. J. Appl. Microbiol. 1998, 84:515-522.
2. Aubert S., Gout E., Bligny R., Douce R. Multiple effects of glycerol on plant cell metabolism: phosphorus-31 nuclear magnetic resonance studies. J. Biol. Chem. 1994, 269:21420-21427.
3. Bailey, R.B., Dimasi, D., Hansen, J.M., Mirrasoul, P.J., Ruecker, C.M., Veeder, G.T., Kaneko, T., Barclay, W.R., 2003. Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermenters. US Patent 6607900.
4. Borowitzka M.A. High-value products from microalgae-their development and commercialisation. J. Appl. Phycol. 2013, 25:743-756.
5. Chang G.F., Gao N.S., Tian G.W., Wu Q.H., Chang M., Wang X.G. Improvement of docosahexaenoic acid production on glycerol by Schizochytrium sp. S31 with constantly high oxygen transfer coefficient. Bioresour. Technol. 2013, 142:400-406.
6. Chang G.F., Luo Z.L., Gu S.T., Wu Q.H., Chang M., Wang X.G. Fatty acid shifts and metabolic activity changes of Schizochytrium sp. S31 cultured on glycerol. Bioresour. Technol. 2013, 142:255-260.
7. Chen X.S., Ren X.D., Dong N., Li S., Li F., Zhao F.L., Tang L., Zhang J.H., Mao Z.G. Culture medium containing glucose and glycerol as a mixed carbon source improves e{open}-poly-l-lysine production by Streptomyces sp. M-Z18. Bioprocess Biosyst. Eng. 2012, 35:469-475.
8. Chi Z., Pyle D., Wen Z., Frear C., Chen S. A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation. Process Biochem. 2007, 42:1537-1545.
9. Chi Z., Liu Y., Frear C., Chen S. Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level. Appl. Microbiol. Biotechnol. 2009, 81:1141-1148.
10. Esterling E.R., French W.T., Hernandez R., Licha M. The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rhodotorula glutinis. Bioresour. Technol. 2009, 100:356-361.
11. Ethier S., Woisard K., Vaughan D., Wen Z. Continuous culture of the microalgae Schizochytrium limacinum on biodiesel-derived crude glycerol for producing docosahexaenoic acid. Bioresour. Technol. 2011, 102:88-93.
12. Hauvermale A., Kuner J., Rosenzweig B., Guerra D., Diltz S., Metz J.G. Fatty acid production in Schizochytrium sp.: involvement of a polyunsaturated fatty acid synthase and a type I fatty acid synthase. Lipids 2006, 41:739-747.
13. Huang T.Y., Lu W.C., Chu I.M. A fermentation strategy for producing docosahexaenoic acid in Aurantiochytrium limacinum SR21 and increasing C22:6 proportions in total fatty acid. Bioresour. Technol. 2012, 123:8-14.
14. 2 limitation. Appl. Microbiol. Biotechnol. 2008, 80:297-306.
15. Mantzouridou F., Naziri E., Tsimidou M.Z. Industrial glycerol as a supplementary carbon source in the production of β-carotene by Blakeslea trispora. J. Agric. Food Chem. 2008, 56:2668-2675.
16. Metz J.G., Roessler P., Facciotti D., Levering C., Dittrich F., Lassner M., Valentine R., Lardizabal K., Domergue F., Yamada A., Yazawa K., Knauf V., Browse J. Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 2001, 293:290-293.
17. Perez-Garcia O., Escalante F.M.E., de-Bashan L.E., Bashan Y. Heterotrophic cultures of microalgae: metabolism and potential products. Water Res. 2011, 45:11-36.
18. Pyle D.J., Garcia R.A., Wen Z. Producing docosahexaenoic acid (DHA)-rich algae from biodiesel-derived crude glycerol: effects of impurities on DHA production and algal biomass composition. J. Agric. Food Chem. 2008, 56:3933-3939.
19. Qu L., Ren L.J., Sun G.N., Ji X.J., Nie Z.K., Huang H. Batch, fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid. Bioprocess Biosyst. Eng. 2013, 36:1905-1912.
20. Ratledge C. Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 2004, 86:807-815.
21. Ren L.J., Sun G.N., Ji X.J., Hu X.C., Huang H. Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp. Bioresour. Technol. 2014, 157:107-113.
22. Riesenberg D., Guthke R. High-cell-density cultivation of microorganisms. Appl. Microbiol. Biotechnol. 1999, 51:422-430.
23. Scott S.D., Armenta R.E., Berryman K.T., Norman A.W. Use of raw glycerol to produce oil rich in polyunsaturated fatty acids by a thraustochytrid. Enzyme Microb. Technol. 2011, 48:267-272.
24. Sun L.N., Ren L.J., Zhang X.Y., Ji X.J., Yan J.C., Huang H. Differential effect of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp. Bioresour. Technol. 2014, 159:199-206.
25. 2 production during mixed carbon (glucose and glycerol) fermentation at pH 7.5 and pH 5.5. Int. J. Hydrogen Energy 2013, 38:3921-3929.
26. Wang Z.Q., Yang S.T. Propionic acid production in glycerol/glucose co-fermentation by Propionibacterium freudenreichii subsp. shermanii. Bioresour. Technol. 2013, 137:116-123.
27. Ward O.P., Singh A. Omega-3/6 fatty acids: alternative sources of production. Process Biochem. 2005, 40:3627-3652.
28. Wu S.T., Yu S.T., Lin L.P. Effect of culture conditions on docosahexaenoic acid production by Schizochytrium sp. S31. Appl. Microbiol. Biotechnol. 2005, 49:72-76.
29. Yokochi T., Honda D., Higashihara T., Nakahara T. Optimization of docosahexaenoic acid production by Aurantiochytrium limacinum SR21. Appl. Microbiol. Biotechnol. 1998, 49:72-76.
30. Yokoyama R., Honda D. Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience 2007, 48:199-211.