Monitoring of hematopoietic chimerism after transplantation for pediatric myelodysplastic syndrome: Real-time or conventional short tandem repeat pcr in peripheral blood or bone marrow?

Biology of Blood and Marrow Transplantation

Volumn 20, Issue 12, 2014, Pages 1918-1925

  Willasch, Andre M ^a   Kreyenberg, Hermann ^a   Shayegi, Nona ^b   Rettinger, Eva ^a   Meyer, Vida ^a   Zabel, Marion ^a   Lang, Peter ^c   Kremens, Bernhard ^d   Meisel, Roland ^e   Strahm, Brigitte ^f   Rossig, Claudia ^g   Gruhn, Bernd ^h   Klingebiel, Thomas ^a   Niemeyer, Charlotte M ^f   Bader, Peter ^a  

^a GOETHE UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

^d UNIVERSITY HOSPITAL ESSEN   (Germany)

^e HEINRICH HEINE UNIVERSITY   (Germany)

^f UNIVERSITY OF FREIBURG   (Germany)

^g UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^h JENA UNIVERSITY HOSPITAL   (Germany)

Author keywords

Allogeneic SCT; Chimerism; Minimal residual disease; Pediatric MDS; Real time PCR

Indexed keywords

BUSULFAN; CYCLOPHOSPHAMIDE; MELPHALAN; OKT 3; THIOTEPA; THYMOCYTE ANTIBODY;

ARTICLE; BLOOD; BONE MARROW; CHILD; CHIMERA; CONTROLLED STUDY; DISEASE COURSE; FALSE POSITIVE RESULT; FEMALE; HUMAN; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MALE; MYELODYSPLASTIC SYNDROME; PATIENT MONITORING; PREDICTIVE VALUE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REFRACTORY ANEMIA WITH EXCESS BLASTS; RELAPSE; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; SHORT TANDEM REPEAT; T CELL DEPLETION; ADOLESCENT; ALLOGRAFT; CLINICAL TRIAL; COMPARATIVE STUDY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HISTOCOMPATIBILITY; INFANT; MULTICENTER STUDY; MYELODYSPLASTIC SYNDROMES; PHYSIOLOGIC MONITORING; PRESCHOOL CHILD; PROCEDURES;

ADOLESCENT; ALLOGRAFTS; BONE MARROW; CHILD; CHILD, PRESCHOOL; FEMALE; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMANS; INFANT; MALE; MONITORING, PHYSIOLOGIC; MYELODYSPLASTIC SYNDROMES; REAL-TIME POLYMERASE CHAIN REACTION; TRANSPLANTATION CHIMERA;

EID: 84912135702     PISSN: 10838791     EISSN: 15236536     Source Type: Journal    
DOI: 10.1016/j.bbmt.2014.07.030     Document Type: Article

References (25)

1. Alizadeh M., Bernard M., Danic B., et al. Quantitative assessment of hematopoietic chimerism after bone marrow transplantation by real-time quantitative polymerase chain reaction. Blood 2002, 99:4618-4625.
2. Hochberg E.P., Miklos D.B., Neuberg D., et al. Anovel rapid single nucleotide polymorphism (SNP)-based method for assessment of hematopoietic chimerism after allogeneic stem cell transplantation. Blood 2003, 101:363-369.
3. Maas F., Schaap N., Kolen S., et al. Quantification of donor and recipient hemopoietic cells by real-time PCR of single nucleotide polymorphisms. Leukemia 2003, 17:621-629.
4. Bai L., Deng Y.M., Dodds A.J., et al. ASYBR green-based real-time PCR method for detection of haemopoietic chimerism in allogeneic haemopoietic stem cell transplant recipients. Eur J Haematol 2006, 77:425-431.
5. Willasch A., Schneider G., Reincke B.S., et al. Sequence polymorphism systems for quantitative real-time polymerase chain reaction to characterize hematopoietic chimerism-high informativity and sensitivity as well as excellent reproducibility and precision of measurement. Lab Hematol 2007, 13:73-84.
6. Gineikiene E., Stoskus M., Griskevicius L. Single nucleotide polymorphism-based system improves the applicability of quantitative PCR for chimerism monitoring. JMol Diagn 2009, 11:66-74.
7. Almeida C.A., Dreyfuss J.L., Azevedo-Shimmoto M.M., et al. Evaluation of 16 SNPs allele-specific to quantify post hSCT chimerism by SYBR green-based qRT-PCR. JClin Pathol 2013, 66:238-242.
8. Koldehoff M., Steckel N.K., Hlinka M., et al. Quantitative analysis of chimerism after allogeneic stem cell transplantation by real-time polymerase chain reaction with single nucleotide polymorphisms, standard tandem repeats, and Y-chromosome-specific sequences. Am J Hematol 2006, 81:735-746.
9. Wiedemann B., Klyuchnikov E., Kroger N., et al. Chimerism studies with quantitative real-time PCR in stem cell recipients with acute myeloid leukemia. Exp Hematol 2010, 38:1261-1271.
10. Chen D.P., Tseng C.P., Wang W.T., et al. Real-time biallelic polymorphism-polymerase chain reaction for chimerism monitoring of hematopoietic stem cell transplantation relapsed patients. Clin Chim Acta 2011, 412:625-630.
11. Martinez-Lopez J., Crooke A., Grande S., et al. Real-time PCR quantification of haematopoietic chimerism after transplantation: a comparison between TaqMan and hybridization probes technologies. Int J Lab Hematol 2010, 32:E17-E25.
12. Willasch A., Eing S., Weber G., et al. Enrichment of cell subpopulations applying automated MACS technique: purity, recovery and applicability for PCR-based chimerism analysis. Bone Marrow Transplant 2010, 45:181-189.
13. Jimenez-Velasco A., Barrios M., Roman-Gomez J., et al. Reliable quantification of hematopoietic chimerism after allogeneic transplantation for acute leukemia using amplification by real-time PCR of null alleles and insertion/deletion polymorphisms. Leukemia 2005, 19:336-343.
14. Thiede C., Bornhauser M., Ehninger G. Strategies and clinical implications of chimerism diagnostics after allogeneic hematopoietic stem cell transplantation. Acta Haematol 2004, 112:16-23.
15. Fehse B., Chukhlovin A., Kuhlcke K., et al. Real-time quantitative Y chromosome-specific PCR (QYCS-PCR) for monitoring hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation. JHematother Stem Cell Res 2001, 10:419-425.
16. Elmaagacli A.H. Real-time PCR for monitoring minimal residual disease and chimerism in patients after allogeneic transplantation. Int J Hematol 2002, 76(Suppl 2):204-205.
17. Wilhelm J., Reuter H., Tews B., et al. Detection and quantification of insertion/deletion variations by allele-specific real-time PCR: application for genotyping and chimerism analysis. Biol Chem 2002, 383:1423-1433.
18. Vago L., Mazzi B., Chiesa L., et al. High-sensitivity hematopoietic chimerism by qPCR for relapse prediction and specific identification of HLA loss leukemic variants. Tissue Antigens 2013, 81:296.
19. Kletzel M., Huang W., Olszewski M., Khan S. Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: real-time PCR (qPCR) versus variable number tandem repeats PCR (VNTR PCR). Chimerism 2013, 4:1-8.
20. Bader P., Niemeyer C., Willasch A., et al. Children with myelodysplastic syndrome (MDS) and increasing mixed chimaerism after allogeneic stem cell transplantation have a poor outcome which can be improved by pre-emptive immunotherapy. Br J Haematol 2005, 128:649-658.
21. Klingebiel T., Niethammer D., Dietz K., Bader P. Progress in chimerism analysis in childhood malignancies-the dilemma of biostatistical considerations and ethical implications. Leukemia 2001, 15:1989-1991.
22. Hasle H., Niemeyer C.M., Chessells J.M., et al. Apediatric approach to the WHO classification of myelodysplastic and myeloproliferative diseases. Leukemia 2003, 17:277-282.
23. Kreyenberg H., Holle W., Mohrle S., et al. Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Tuebingen experience. Leukemia 2003, 17:237-240.
24. Fry T.J., Willasch A., Bader P. The graft-versus-tumor effect in pediatric malignancy. Pediatr Clin North Am 2010, 57:67-81.
25. Bader P., Kreyenberg H., Hoelle W., et al. Increasing mixed chimerism is an important prognostic factor for unfavorable outcome in children with acute lymphoblastic leukemia after allogeneic stem-cell transplantation: possible role for pre-emptive immunotherapy?. JClin Oncol 2004, 22:1696-1706.