Enrichment of cell subpopulations applying automated MACS technique: Purity, recovery and applicability for PCR-based chimerism analysis

Bone Marrow Transplantation

Volumn 45, Issue 1, 2010, Pages 181-189

  Willasch, A ^a   Eing, S ^a   Weber, G ^a   Kuci S ^a   Schneider, G ^a   Soerensen, J ^a   Jarisch, A ^a   Rettinger, E ^a   Koehl, U ^a   Klingebiel, T ^a   Kreyenberg, H ^a   Bader, P ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Immunomagnetic beads; Lineage specific chimerism; MACS; SNP qPCR; STR PCR; Subpopulations

Indexed keywords

CD14 ANTIGEN; CD15 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD56 ANTIGEN;

ADOLESCENT; ADULT; ARTICLE; AUTOMATION; BLOOD SAMPLING; CELL ISOLATION; CELL SELECTION; CELL SORTER; CELL SUBPOPULATION; CHILD; CHIMERA; CLINICAL ARTICLE; FEMALE; HUMAN; HUMAN CELL; IMMUNOMAGNETIC SEPARATION; IMMUNOPHENOTYPING; MAGNETIC CELL SORTING; MALE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; ANTIGENS, CD14; ANTIGENS, CD15; ANTIGENS, CD19; ANTIGENS, CD3; ANTIGENS, CD56; CELL LINEAGE; CELL SEPARATION; CHILD; CHILD, PRESCHOOL; FEMALE; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMANS; LYMPHOCYTE SUBSETS; MAGNETICS; MALE; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POSTOPERATIVE CARE; TANDEM REPEAT SEQUENCES; TRANSPLANTATION CHIMERA;

EID: 75149122851     PISSN: 02683369     EISSN: 14765365     Source Type: Journal    
DOI: 10.1038/bmt.2009.89     Document Type: Article

References (28)

1. Bader P, Kreyenberg H, Hoelle W, Dueckers G, Handgretinger R, Lang P et al.. Increasing mixed chimerism is an important prognostic factor for unfavorable outcome in children with acute lymphoblastic leukemia after allogeneic stem-cell transplantation: possible role for pre-emptive immunotherapy? J Clin Oncol. 2004; 22: 1696-1706.
2. Willasch A, Hoelle W, Kreyenberg H, Niethammer D, Handgretinger R, Lang P et al.. Outcome of allogeneic stem cell transplantation in children with non-malignant diseases. Haematologica 2006; 91: 788-794.
3. Thiede C, Bornhauser M, Ehninger G. Strategies and clinical implications of chimerism diagnostics after allogeneic hematopoietic stem cell transplantation. Acta Haematol 2004; 112: 16-23.
4. Liesveld JL, Rothberg PG. Mixed chimerism in SCT: conflict or peaceful coexistence? Bone Marrow Transplant 2008; 42: 297-310.
5. Saito B, Fukuda T, Yokoyama H, Kurosawa S, Takahashi T, Fuji S et al.. Impact of T cell chimerism on clinical outcome in 117 patients who underwent allogeneic stem cell transplantation with a busulfan-containing reduced-intensity conditioning regimen. Biol Blood Marrow Transplant 2008; 14: 1148-1155.
6. Ozyurek E, Cowan MJ, Koerper MA, Baxter-Lowe LA, Dvorak CC, Horn BN. Increasing mixed chimerism and the risk of graft loss in children undergoing allogeneic hematopoietic stem cell transplantation for non-malignant disorders. Bone Marrow Transplant 2008; 42: 83-91.
7. Lion T, Muller-Berat N. Chimerism testing after allogeneic stem cell transplantation: importance of timing and optimal technique for testing in different clinical-biological situations. Leukemia 2003; 17: 612. (Pubitemid 36395656)
8. Matthes-Martin S, Lion T, Haas OA, Frommlet F, Daxberger H, Konig M et al.. Lineage-specific chimaerism after stem cell transplantation in children following reduced intensity conditioning: potential predictive value of NK cell chimaerism for late graft rejection. Leukemia 2003; 17: 1934-1942.
9. Lim ZY, Pearce L, Ingram W, Ho AY, Mufti GJ, Pagliuca A. Chimerism does not predict for outcome after alemtuzumabbased conditioning: lineage-specific analysis of chimerism of specific diseases may be more informative. Bone Marrow Transplant 2008; 41: 587-588.
10. Ringden O. Immunotherapy by allogeneic stem cell transplantation. Adv Cancer Res 2007; 97C: 25-60.
11. Lion T. Detection of impending graft rejection and relapse by lineage-specific chimerism analysis. Methods Mol Med 2007; 134: 197-216.
12. Miura Y, Tanaka J, Toubai T, Tsutsumi Y, Kato N, Hirate D et al.. Analysis of donor-type chimerism in lineage-specific cell populations after allogeneic myeloablative and non-myeloablative stem cell transplantation. Bone Marrow Transplant. 2006; 37: 837-843.
13. Zeiser R, Spyridonidis A, Wasch R, Ihorst G, Grullich C, Bertz H et al.. Evaluation of immunomodulatory treatment based on conventional and lineage-specific chimerism analysis in patients with myeloid malignancies after myeloablative allogeneic hematopoietic cell transplantation. Leukemia 2005; 19: 814-821.
14. Bader P, Stoll K, Huber S, Geiselhart A, Handgretinger R, Niemeyer C et al.. Characterization of lineage-specific chimaerism in patients with acute leukaemia and myelodysplastic syndrome after allogeneic stem cell transplantation before and after relapse. Br J Haematol 2000; 108: 761-768.
15. Breuer S, Matthes S, König M, Fritsch G, Pötschger U, Lawitschka A et al.. Recipient chimerism in the T-cell lineage is indicative of impending graft rejection in pediatric patients undergoing allogeneic hematopoietic stem cell transplantation. In: 40th Congress of SIOP, abstract book. Wiley-Liss Inc.: Hoboken, NY, USA, 2008, pp 60-61.
16. Pongers-Willemse MJ, Verhagen OJ, Tibbe GJ, Wijkhuijs AJ, De Haas V, Roovers E et al.. Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes. Leukemia 1998; 12: 2006-2014.
17. Kreyenberg H, Holle W, Mohrle S, Niethammer D, Bader P. Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Tuebingen experience. Leukemia 2003; 17: 237-240.
18. Watzinger F, Lion T, Steward C. The RSD code: proposal for a nomenclature of allelic configurations in STR-PCR-based chimerism testing after allogeneic stem cell transplantation. Leukemia 2006; 20: 1448-1452.
19. Scharf SJ, Smith AG, Hansen JA, McFarland C, Erlich HA. Quantitative determination of bone marrow transplant engraftment using fluorescent polymerase chain reaction primers for human identity markers. Blood 1995; 85: 1954-1963.
20. Willasch A, Schneider G, Reincke BS, Shayegi N, Kreyenberg H, Kuci S et al.. Sequence polymorphism systems for quantitative real-time polymerase chain reaction to characterize hematopoietic chimerism-high informativity and sensitivity as well as excellent reproducibility and precision of measurement. Lab Hematol 2007; 13: 73-84.
21. Steinbach D, Debatin KM. What do we mean by sensitivity when we talk about detecting minimal residual disease? Leukemia 2008; 22: 1638-1639.
22. The R Foundation for Statistical Computing c/o Institut für Statistik und Wahrscheinlichkeitstheorie der Technischen Universität Wien. R, a language and environment for statistical computing and graphics. University of Vienna, Austria 2003.
23. Vitale M, Della CM, Carlomagno S, Romagnani C, Thiel A, Moretta L. et al.. The small subset of CD56brightCD16- natural killer cells is selectively responsible for both cell proliferation and interferon-gamma production upon interaction with dendritic cells. Eur J Immunol 2004; 34: 1715-1722.
24. Romagnani C, Juelke K, Falco M, Morandi B, D'Agostino A, Costa R et al.. CD56brightCD16- killer Ig-like receptor- NK cells display longer telomeres and acquire features of CD56dim NK cells upon activation. J Immunol 2007; 178: 4947-4955.
25. Beck O, Seidl C, Lehrnbecher T, Kreyenberg H, Schwabe D, Klingebiel T et al.. Quantification of chimerism within peripheral blood, bone marrow and purified leukocyte subsets: comparison of singleplex and multiplex PCR amplification of short tandem repeat (STR) loci. Eur J Haematol. 2006; 76: 237-244.
26. Tomblyn M, Lazarus HM. Donor lymphocyte infusions: the long and winding road: how should it be traveled? Bone Marrow Transplant 2008.
27. Klingebiel T, Bader P. Delayed lymphocyte infusion in children given SCT. Bone Marrow Transplant 2008; 41 (Suppl 2): S23-S26.
28. Formankova R, Sedlacek P, Krskova L, Rihova H, Sramkova L, Star J. Chimerism-directed adoptive immunotherapy in prevention and treatment of post-transplant relapse of leukemia in childhood. Haematologica 2003; 88: 117-118.