Down-regulation of sweetpotato lycopene β-cyclase gene enhances tolerance to abiotic stress in transgenic calli

Molecular Biology Reports

Volumn 41, Issue 12, 2014, Pages 8137-8148

  Kim, Sun Ha ^a   Jeong, Jae Cheol ^a   Park, Seyeon ^a   Bae, Ji Yeong ^b   Ahn, Mi Jeong ^b   Lee, Haeng Soon ^a   Kwak, Sang Soo ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Gyeongsang National University   (South Korea)

Author keywords

Carotenoid; Drought stress; Lycopene cyclase; Metabolic engineering; RNAi; Salt stress; Sweetpotato

Indexed keywords

ABSCISIC ACID; CAROTENOID; CYTOCHROME P450; MALONALDEHYDE; ISOMERASE; LYCOPENE; LYCOPENE CYCLASE-ISOMERASE; MACROGOL DERIVATIVE; VEGETABLE PROTEIN;

ABIOTIC STRESS; ANTIOXIDANT ACTIVITY; ARTICLE; CALLUS (PLANT); CAROTENOID CLEAVAGE DIOXIGENASE 1 GENE; CHY BETA GENE; CONTROLLED STUDY; CYTOCHROME P450 GENE; DOWN REGULATION; DROUGHT STRESS; GENE ACTIVATION; LYCOPENE BETA CYCLASE GENE; NONHUMAN; OXIDATIVE STRESS; PLANT CELL; PLANT GENE; PLANT METABOLISM; PLANT RESPONSE; SALT STRESS; SEQUENCE ANALYSIS; SWEET POTATO; TRANSGENIC PLANT; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGICAL STRESS; SALT TOLERANCE;

IPOMOEA BATATAS; LYCOPERSICON ESCULENTUM;

ABSCISIC ACID; CAROTENOIDS; DOWN-REGULATION; GENE EXPRESSION REGULATION, PLANT; INTRAMOLECULAR LYASES; IPOMOEA BATATAS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYETHYLENE GLYCOLS; SALT-TOLERANCE; STRESS, PHYSIOLOGICAL;

EID: 84912049440     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-014-3714-4     Document Type: Article

References (40)

1. Cunningham FX, Pogson B, Sun Z, McDonald KA, DellaPenna D, Gantt E (1996) Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8(9):1613–1626
2. Cunningham FX, Gantt E (1998) Genes and enzymes of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 49:557–583
3. Goodwin T (1980) The biochemistry of the carotenoids, vol 1(2nd edn). Chapman & Hall, London
4. Ronen GCM, Zamir D, Hirschberg J (1999) Regulation of carotenoid biosynthesis during tomato fruit development: expression of the gene for lycopene epsilon-cyclase is down-regulated during ripening and is elevated in the mutant Delta. Plant J 17:341–351
5. Giovannucci E, Rimm E, Liu Y, Stampfer M, Willett W (2002) A prospective study of tomato products, lycopene, and prostate cancer risk. J Natl Cancer Inst 94:391–398
6. Ronen G, Carmel-Goren L, Zamir D, Hirschberg J (2000) An alternative pathway to beta-carotene formation in plant chromoplasts discovered by map-based cloning of Beta and old-gold color mutations in tomato. Proc Natl Acad Sci USA 97:11102–11107
7. Rosati C, Aquilani R, Dharmapuri S, Pallara P, Marusic C, Tavazza R, Bouvier F, Camara B, Giuliano G (2000) Metabolic engineering of beta-carotene and lycopene content in tomato fruit. Plant J 24(3):413–420
8. Hugueney P, Badillo A, Chen H-C, Klein A, Hirschberg J, Camara B, Kuntz M (1995) Metabolism of cyclic carotenoids: a model for the alteration of this biosynthetic pathway in Capsicum annuum chromoplasts. Plant J 8(3):417–424
9. Alquézar B, Zacarías L, Rodrigo MJ (2009) Molecular and functional characterization of a novel chromoplast-specific lycopene β-cyclase from Citrus and its relation to lycopene accumulation. J Exp Bot 60(6):1783–1797
10. Devitt LC, Fanning K, Dietzgen RG, Holton TA (2010) Isolation and functional characterization of a lycopene β-cyclase gene that controls fruit colour of papaya (Carica papaya L.). J Exp Bot 61(1):33–39
11. Pecker I, Gabbay R, Cunningham F Jr, Hirschberg J (1996) Cloning and characterization of the cDNA for lycopene β-cyclase from tomato reveals decrease in its expression during fruit ripening. Plant Mol Biol 30(4):807–819
12. Ziska LHRG, Tomecek M, Prior SA, Torbet HA, Sicher R (2009) An evaluation of cassava, sweetpotato and field corn as potential carbohydrate sources for bioethanol production in Alabama and Maryland. Biomass Bioenergy 33:1503–1508
13. Kim SH, Ahn YO, Ahn M-J, Jeong JC, Lee H-S, Kwak S-S (2013) Downregulation of the lycopene epsilon-cyclase gene increases carotenoid synthesis via the β-branch-specific pathway and enhances salt-stress tolerance in sweetpotato transgenic calli. Physiol Plant 147:432–442
14. Kim SH, Ahn YO, Ahn M-J, Lee H-S, Kwak S-S (2012) Down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato. Phytochemistry 74:69–78
15. Hofgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucl Acids Res 16:9877
16. Lim CJ, Kim HY, Lee CH, Kim Y, Back K, Bae J-M, Lee SW, Ahn M-J (2009) Variation in carotenoid composition in carrots during storage and cooking. J Food Sci Nutr 14:240–245
17. Pieroni A, Janiak V, Durr CM, Ludeke S, Trachsel E, Heinrich M (2002) In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy. Phytother Res 16(5):467–473
18. Chadwick CA, Potten CS, Nikaido O, Matsunaga T, Proby C, Young AR (1995) The detection of cyclobutane thymine dimers (6-4) photolesions and the dewar photoisomers in sections of UV-irradiated human skin using specific antibodies, and the demonstration of depth penetration effects. J Photochem Photobiol B 28(2):163–170
19. Verslues PEAM, Katiyar-Agarwal S, Zhu J, Zhu J-K (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539
20. Zhao LZF, Guo J, Yang Y, Li B, Zhang L (2004) Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed. Plant Physiol 134:849–857
21. Peever TLHV (1989) Electrolyte leakage, lipoxygenase, and lipid peroxidation induced in tomato leaf tissue by specific and nonspecific elicitors from Cladosporium fulvum. Plant Physiol 90:867–875
22. Artsaenko OPM, Zur Nieden U, Fiedler U, Weiler EW, Müntz K, Conrad U (1995) Expression of a single-chain Fv antibody against abscisic acid creates a wilty phenotype in transgenic tobacco. Plant J 8:745–750
23. Moreno JC, Pizarro L, Fuentes P, Handford M, Cifuentes V, Stange C (2013) Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota. PLoS One 8(3):e58144
24. Beyer P, Kröncke U, Nievelstein V (1991) On the mechanism of the lycopene isomerase/cyclase reaction in Narcissus pseudonarcissus L. chromoplasts. J Biol Chem 266(26):17072–17078
25. Apel W, Bock R (2009) Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiol 151(1):59–66
26. Ahrazem O, Rubio-Moraga A, López RC, Gómez-Gómez L (2010) The expression of a chromoplast-specific lycopene beta cyclase gene is involved in the high production of saffron’s apocarotenoid precursors. J Exp Bot 61(1):105–119
27. Bai L, Kim E-H, DellaPenna D, Brutnell TP (2009) Novel lycopene epsilon cyclase activities in maize revealed through perturbation of carotenoid biosynthesis. Plant J 59(4):588–599
28. Bang H, Kim S, Leskovar D, King S (2007) Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene β-cyclase (LCYB) gene. Mol Breed 20(1):63–72
29. Chaudhary N, Nijhawan A, Khurana J, Khurana P (2010) Carotenoid biosynthesis genes in rice: structural analysis, genome-wide expression profiling and phylogenetic analysis. Mol Genet Genomics 283(1):13–33
30. Lange BM, Ghassemian M (2003) Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism. Plant Mol Biol 51(6):925–948
31. Just BJ, Santos CAF, Fonseca MEN, Boiteux LS, Oloizia BB, Simon PW (2007) Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers and genome mapping. Theor Appl Genet 114(4):693–704
32. Giuliano G, Al-Babili S, von Lintig J (2003) Carotenoid oxygenases: cleave it or leave it. Trends Plant Sci 8(4):145–149
33. Rodríguez-Ávila NL, Narváez-Zapata JA, Ramírez-Benítez JE, Aguilar-Espinosa ML, Rivera-Madrid R (2011) Identification and expression pattern of a new carotenoid cleavage dioxygenase gene member from Bixa orellana. J Exp Bot 62(15):5385–5395
34. Luo Y, Liu YB, Dong YX, Gao X-Q, Zhang XS (2009) Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustment. J Plant Physiol 166(4):385–394
35. Chen X, Han H, Jiang P, Nie L, Bao H, Fan P, Lv S, Feng J, Li Y (2011) Transformation of β-lycopene cyclase genes from Salicornia europaea and Arabidopsis conferred salt tolerance in Arabidopsis and Tobacco. Plant Cell Physiol 52(5):909–921
36. Burton GW, Ingold KU (1984) beta-carotene: an unusual type of lipid antioxidant. Science 224:569–573
37. Krinsky NI, Yeum KJ (2003) Carotenoid-radical interactions. Biochem Biophys Res Commun 305:754–760
38. El-Agamey ALG, McGarvey DJ, Mortensen A, Phillip DM, Truscott TG, Young AJ (2004) Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys 430:37–48
39. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53(1):247–273
40. Li XJ, Yang MF, Chen H, Qu LQ, Chen F (1804) Shen SH (2010) Abscisic acid pretreatment enhances salt tolerance of rice seedlings: proteomic evidence. Biochim Biophys Acta 4:929–940