Structure-function analyses of cytochrome P450revI involved in reveromycin A biosynthesis and evaluation of the biological activity of its substrate, reveromycin T

Journal of Biological Chemistry

Volumn 289, Issue 47, 2014, Pages 32446-32458

  Takahashi, Shunji ^a,b   Nagano, Shingo ^c,d   Nogawa, Toshihiko ^a   Kanoh, Naoki ^e   Uramoto, Masakazu ^b   Kawatani, Makoto ^b   Shimizu, Takeshi ^b   Miyazawa, Takeshi ^b   Shiro, Yoshitsugu ^d   Osada, Hiroyuki ^a,b  

^a Elements Chemistry Laboratory   (Japan)

^b RIKEN   (Japan)

^c TOTTORI UNIVERSITY   (Japan)

^d RIKEN SPring 8 Center   (Japan)

^e TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; BIOSYNTHESIS; CRYSTAL STRUCTURE; ESTERIFICATION; ESTERS; GENES; HYDROXYLATION; METABOLITES; SUBSTRATES;

ANTICANCER ACTIVITIES; BIOSYNTHETIC GENE CLUSTER; COCRYSTAL STRUCTURE; PHYSIOLOGICAL SUBSTRATE; SECONDARY METABOLITES; SITE DIRECTED MUTAGENESIS; STRUCTURAL STUDIES; STRUCTURE-FUNCTION ANALYSIS;

ENZYME ACTIVITY;

ANTINEOPLASTIC AGENT; CARBOXYL GROUP; CYTOCHROME P450; REVEROMYCIN A; REVEROMYCIN T; UNCLASSIFIED DRUG; CYTOCHROME P-450 107E6 PROTEIN, HUMAN; ISOLEUCINE TRANSFER RNA LIGASE; PROTEIN BINDING; PYRAN DERIVATIVE; SPIRO COMPOUND;

ANTINEOPLASTIC ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; BIOSYNTHESIS; CATALYSIS; CRYSTAL STRUCTURE; DRUG SCREENING; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SUBSTRATE; GENE CLUSTER; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC VARIABILITY; HYDROXYLATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOCATALYSIS; CELL CULTURE; CELL SURVIVAL; CHEMICAL STRUCTURE; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; ENZYME SPECIFICITY; GENETICS; HELA CELL LINE; HUMAN; K562 CELL LINE; KINETICS; METABOLISM; MICROBIAL VIABILITY; MUTATION; OSTEOCLAST; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN TERTIARY STRUCTURE; TANDEM MASS SPECTROMETRY; TUMOR CELL LINE; X RAY CRYSTALLOGRAPHY;

ANIMALS; BIOCATALYSIS; CELL LINE, TUMOR; CELL SURVIVAL; CELLS, CULTURED; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HELA CELLS; HUMANS; HYDROXYLATION; ISOLEUCINE-TRNA LIGASE; K562 CELLS; KINETICS; MICROBIAL VIABILITY; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTATION; OSTEOCLASTS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PYRANS; SPIRO COMPOUNDS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

EID: 84911931679     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.598391     Document Type: Article

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