Single stage aqueous two-phase extraction for monoclonal antibody purification from cell supernatant

Fluid Phase Equilibria

Volumn 385, Issue , 2015, Pages 227-236

  Muendges, Jan ^a   Stark, Ina ^a   Mohammad, Sultan ^a   Górak, Andrzej ^a,b   Zeiner, Tim ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

^b LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Aqueous two phase system; Cell supernatent; Extraction; Monoclonal antibody

Indexed keywords

CELLS; CYTOLOGY; EXTRACTION; MONOCLONAL ANTIBODIES; PURIFICATION; SODIUM; SODIUM CHLORIDE; SOLUBILITY; SOLVENT EXTRACTION;

AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE EXTRACTION; CHINESE HAMSTER OVARY CELLS; EXTRACTION CONDITIONS; MONOCLONAL ANTIBODY PURIFICATION; OPERATING WINDOWS; PURIFICATION FACTORS; SINGLE EXTRACTION;

LOADING;

EID: 84911915102     PISSN: 03783812     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fluid.2014.10.034     Document Type: Article

References (17)

1. Igarashi, Kieckbusch T.G., Franco T.T. Bioprocess. Biosyst. Eng. 2004, 26:151-157.
2. Albertsson P.-Å. Partition of cell particles and macromolecules. Separation and Purification of Biomolecules, Cell Organelles, Membranes, and Cells in Aqueous Polymer Two-phase Systems and Their Use in Biochemical Analysis and Biotechnology 1986, Wiley, New York. 3rd ed.
3. Andrews B.A., Head D.M., Dunthorne P., Asenjo J.A. Biotechnol. Tech. 1990, 4:49-54.
4. Andrews B.A., Nielsen S., Asenjo J.A. Bioseparations 1996, 6:303-313.
5. Rosa P.A.J., Azevedo A.M., Aires-Barros M.R. J. Chromatogr. A 2007, 1141:50-60.
6. Azevedo A.M., Rosa P.A.J., Ferreira I.F., Aires-Barros M.R. J. Biotechnol. 2007, 132:209-217.
7. Azevedo A.M., Gomes A.G., Rosa P.A.J., Ferreira I.F., Pisco A.M.M.O., Aires-Barros M.R. Sep. Purif. Technol. 2009, 65:14-21.
8. Platis D., Labrou N.E. J. Chromatogr. A 2006, 1128:114-124.
9. Rito-Palomares M., Haynes C.A. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 2004, 807:3-11.
10. Rosa P.A.J., Ferreira I.F., Azevedo A.M., Aires-Barros M.R. J. Chromatogr. A 2010, 1217:2296-2305.
11. Oelmeier S.A., Dismer F., Hubbuch J. Biotechnol. Bioeng. 2011, 108:69-81.
12. Benavides J., Rito-Palomares M. J. Chem. Technol. Biotechnol. 2008, 83:133-142.
13. Hustedt H., Kroner K.H., Kula M.R. Partitioning in aqueous two-phase systems. Theory, Methods, Uses, and Application to Biotechnology 1985, 529-589. Academic Press, Orlando. D.E. Walter (Ed.).
14. Prinz A., Zeiner T., Vössing T., Schüttmann I., Zorn H., Górak A. Chem. Eng. Trans. 2012, 27:349-354.
15. Prinz A., Hönig J., Schüttmann I., Zorn H., Zeiner T. Proc. Biochem. 2014, 49:335-346.
16. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Anal. Biochem. 1985, 150:76-85.
17. González-González M., Mayolo-Deloisa K., Rito-Palomares M., Winkler R. Proc. Biochem. 2011, 46:413-417.