Experimental investigation of laccase purification using aqueous two-phase extraction

Chemical Engineering Transactions

Volumn 27, Issue , 2012, Pages 349-354

  Prinz, Axel ^a   Zeiner, Tim ^a   Vössing, Thomas ^a   Schüttmann, Ina ^b   Zorn, Holger ^b   Górak, Andrzej ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; EXTRACTION; SODIUM; SODIUM CHLORIDE; SODIUM COMPOUNDS; SOLVENT EXTRACTION;

AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE EXTRACTION; CHLORIDE CONCENTRATIONS; CULTURE SUPERNATANT; EXPERIMENTAL INVESTIGATIONS; LACCASES; PLEUROTUS; SODIUM PHOSPHATE;

ENZYMES;

EID: 84864405400     PISSN: 22839216     EISSN: 22839216     Source Type: Journal    
DOI: 10.3303/CET1227059     Document Type: Conference Paper

References (12)

1. Albertsson P.A., 1985, Partition of cell particles and macromolecules, John Wiley & Sons, New York, United States.
2. Beijerinck M.W., 1910, On the emulsion formation during mixing of aqueous solutions of certain colloids gelatinierender (In German), Colloid & Polymer Science, 7(1), 16-20.
3. Cabezas jr. H., 1996, Theory of phase formation in aqueous two-phase systems, Journal of Chromatography B, 680, 3-30.
4. Kim C.W., Rha C., 2000. Interfacial Tension of Polyethylene Glycol/Potassium Phosphate Aqueous Two-Phase Systems, Physics and Chemistry of Liquids, 38, 25-34.
5. Kroner K.H., Hustedt H., Granda S., Kula M.R., 1978, Technical Aspects of Separation Using Aqueous Two-Phase Systems in Enzyme Isolation Processes, Biotechnology and Bioengineering, 20, 1967-1978.
6. Linke D., Bouws H., Peters T., Nimitz M., Berger R.G., Zorn H., 2005, Laccase of Pleurotus sapidus: Characterization and Cloning, Journal of Agricultural and Food Chemistry, 53, 9498-9505.
7. Majcherczyk A., Johannes C., Hüttermann A., 1999, Oxidation of aromatic alcohols by laccase from Trametes versicolor mediated by the 2,2'-azino-bis- (3-ethylbenzothiazoline-6-sulphonic acid) cation radical and dication, Journal of Applied Microbiology, 51, 267-276.
8. Salamanca M.H., Merchuk J.C., Andrews B.A., Asenjo J.A., 1998, On the kinetics of phase separation in aqueous two-phase systems, Journal of Chromatography B, 711, 319-329.
9. Salabat A., Moghadam S.T., Far M.R., 2010, Liquid-liquid equilibria of aqueous two-phase systems composed of TritonX-100 and sodium citrate or magnesium sulfate salts, Computer Coupling of Phase Diagrams and Thermochemistry, 34, 81-83.
10. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Proventano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C., 1985, Measurement of Protein Using Bicinchoninic Acid, Analytical Biochemistry, 150, 76-85.
11. Solano-Castillo C., Rito-Palomares M., 2000, Kinetics of phase separation under different process and design parameters in aqueous two-phase systems, Journal of Chromatography B, 743, 195-201.
12. Yoon S.-H., Robyt J.F., 2005, Activation and stabilization of 10 starch degrading enzymes by Triton X-100, polyethylene glycols, and polyvinyl alcohols, Enzyme and Microbial Technology, 37, 556-562.