Comparison of the FilmArray assay and in-house real-time PCR for detection of respiratory infection

Scandinavian Journal of Infectious Diseases

Volumn 46, Issue 12, 2014, Pages 897-901

  Andersson, Maria E ^a   Olofsson, Sigvard ^a   Lindh, Magnus ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Acute respiratory infection; FilmArray; Molecular diagnostics; Point of care testing; Real time PCR

Indexed keywords

ADENOVIRUS; ARTICLE; BACTERIUM DETECTION; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FILMARRAY RESPIRATORY PANEL ASSAY; GENE AMPLIFICATION; HEALTH CARE COST; HUMAN; MAJOR CLINICAL STUDY; MICROORGANISM DETECTION; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; NUCLEIC ACID ANALYSIS; POINT OF CARE TESTING; POLYMERASE CHAIN REACTION SYSTEM; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT EXAMINATION; RESPIRATORY TRACT INFECTION; VIRUS DETECTION; ACUTE DISEASE; ADOLESCENT; ADULT; BACTERIUM; CHILD; COMPARATIVE STUDY; DIAGNOSTIC KIT; GENETICS; HOSPITAL INFORMATION SYSTEM; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PROCEDURES; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; TIME; VERY ELDERLY; VIRUS; YOUNG ADULT;

DIAGNOSTIC KIT; PRIMER DNA;

ACUTE DISEASE; ADOLESCENT; ADULT; AGED, 80 AND OVER; BACTERIA; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; HUMANS; INFANT; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; POINT-OF-CARE SYSTEMS; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIRUSES; YOUNG ADULT;

EID: 84911444424     PISSN: 00365548     EISSN: 16511980     Source Type: Journal    
DOI: 10.3109/00365548.2014.951681     Document Type: Article

References (17)

1. Olofsson S, Brittain-Long R, Andersson L.-M, Westin J, Lindh M, PCR for detection of respiratory viruses: Seasonal variations of virus infections Exp Rev Anti Infect Ther 2011 9 615-615
2. Mahony JB. D etection of respiratory viruses by molecular methods. Clin Microbiol Rev 2008; 21: 716-47.
3. Gadsby NJ, Hardie A, Claas ECJ, T empleton KE. C omparison of the Luminex Respiratory Virus Panel Fast Assay with in-house real-Time PCR for respiratory viral infection diagnosis. J Clin Microbiol 2010; 48: 2213-16.
4. Popowitch EB, O 'Neill SS, Miller MB. C omparison of the Biofi re FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP Fast Multiplex Assays for detection of respiratory viruses. J Clin Microbiol 2013; 51: 1528-33.
5. Heim A, Ebnet C, Harste G, Pring- Åkerblom P. Rapid and quantitative detection of human adenovirus DNA by realtime PCR. J Med Virol 2003; 70: 228-39.
6. Gunson RN, Collins TC, Carman WF. R eal-Time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J Clin Virol 2005; 33: 341-4.
7. Brittain-Long R, Nord S, Olofsson S, Westin J, Anderson L-M, Lindh M. Multiplex real-Time PCR for detection of respiratory tract infections. J Clin Virol 2008; 41: 53-6.
8. Ward CL, Dempsey MH, Ring CJA, K empson RE, Zhang L, Gor D, et al. Design and performance testing of quantitative real time PCR assays for infl uenza A and B viral load measurement. J Clin Virol 2004; 29: 179-88.
9. Watzinger F, Suda M, Preuner S, Baumgartinger R, Ebner K, Baskova L, et al. Real-Time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. J Clin Microbiol 2004; 42: 5189-98.
10. Poritz M A, Blaschke AJ, Byington C L, Meyers L, Nilsson K, Jones DE, et al. FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS One 2011; 6: e26047.
11. Renaud C, Crowley J, Jerome KR, Kuypers J. Comparison of FilmArray Respiratory Panel and laboratory-developed real-Time reverse transcription-polymerase chain reaction assays for respiratory virus detection. Diagn Microbiol Infect Dis 2012; 74: 379-83.
12. Pierce VM, Elkan M, Leet M, McGowan KL, Hodinka RL. Comparison of the Idaho Technology FilmArray system to real-Time PCR for detection of respiratory pathogens in children. J Clin Microbiol 2012; 50: 364-71.
13. Couturier MR, Barney T, Alger G, Hymas WC, Stevenson JB, Hillyard D, et al. Evaluation of the FilmArray Respiratory Panel for clinical use in a large children's hospital. J Clin Lab Anal 2013; 27: 148-54.
14. Don M, Soderlund-Venermo M, Hedman K, Ruuskanen O, Allander T, Korppi M. Don't forget serum in the diagnosis of human bocavirus infection. J Infect Dis 2011; 203: 1031-2.
15. Zhao B, Y u X, Wang C, Teng Z, Wang C, Shen J, et al. High human bocavirus viral load is associated with disease severity in children under fi ve years of age. PLoS One 2013; 8: e62318.
16. L oeffelholz MJ, Pong DL, Pyles RB, Xiong Y, Miller A L, Bufton KK, et al. Comparison of the FilmArray Respiratory Panel and Prodesse Real-Time PCR assays for detection of respiratory pathogens. J Clin Microbiol 2011; 49: 4083-8.
17. Moore C. Point-of-care tests for infection control: should rapid testing be in the laboratory or at the front line? J Hosp Infect 2013; 85: 1-7.