Isolation of primary murine brain microvascular endothelial cells

Journal of Visualized Experiments

Volumn , Issue 93, 2014, Pages

  Ruck, Tobias ^a   Bittner, Stefan ^a,b   Epping, Lisa ^a   Herrmann, Alexander M ^a   Meuth, Sven G ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b Interdisciplinary Center for Clinical Research IZKF Münster   (Germany)

Author keywords

Blood brain barrier; Central nervous system; Endothelial cells; Immune cell trafficking; Issue 93; Neurodegeneration; Neuroinflammation; Neuroscience; Neurovascular unit

Indexed keywords

ANIMAL; ANIMAL MODEL; BLOOD BRAIN BARRIER; BRAIN; C57BL MOUSE; CULTURE TECHNIQUE; CYTOLOGY; ENDOTHELIUM CELL; HOMEOSTASIS; METABOLISM; MOUSE; PROCEDURES; TIGHT JUNCTION; TRANSPORT AT THE CELLULAR LEVEL; VASCULARIZATION;

ANIMALS; BIOLOGICAL TRANSPORT; BLOOD-BRAIN BARRIER; BRAIN; CELL CULTURE TECHNIQUES; ENDOTHELIAL CELLS; HOMEOSTASIS; MICE; MICE, INBRED C57BL; MODELS, ANIMAL; TIGHT JUNCTIONS;

EID: 84911381441     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52204     Document Type: Article

References (26)

1. Neuwelt, E. A., et al. Engaging neuroscience to advance translational research in brain barrier biology. Nature reviews. Neuroscience. 12, 169-182 (2011).
2. Ohtsuki, S., Terasaki, T. Contribution of carrier-mediated transport systems to the blood-brain barrier as a supporting and protecting interface for the brain; importance for CNS drug discovery and development. Pharmaceutical Research. 24, 1745-1758 (2007).
3. Bittner, S., et al. Endothelial TWIK-related potassium channel-1 (TREK1) regulates immune-cell trafficking into the CNS. Nature Medicine. 19, 1161-1165 (2013).
4. Zlokovic, B. V. The blood-brain barrier in health and chronic neurodegenerative disorders.Neuron. 57, 178-201 (2008).
5. Stoll, G., et al. Transient widespread blood-brain barrier alterations after cerebral photothrombosis as revealed by gadofluorine M-enhanced magnetic resonance imaging. Journal of Cerebral Blood Flow & Metabolism. 29, 331-341 (2009).
6. Kleinschnitz, C., et al. Glucocorticoid insensitivity at the hypoxic blood-brain barrier can be reversed by inhibition of the proteasome. Stroke. 42, 1081-1089 (2011).
7. Hawkins, B. T., Davis, T. P. The blood-brain barrier/neurovascular unit in health and disease. Pharmacological reviews. 57, 173-185 (2005).
8. Bazzoni, G., Dejana, E. Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis. Physiological reviews. 84, 869-901 (2004).
9. Pardridge, W. M. Blood-brain barrier delivery. Drug Discovery Today. 12, 54-61 (2007).
10. Persidsky, Y., Ramirez, S. H., Haorah, J., Kanmogne, G. D. Blood-brain barrier: structural components and function under physiologic and pathologic conditions. Journal of neuroimmunepharmacology : the official journal of the Society on NeuroImmune Pharmacology. 1, 223-236 (2006).
11. Pardridge, W. M. Molecular biology of the blood-brain barrier. Molecular Biotechnology. 30, 57-70 (2005).
12. Ley, K., Laudanna, C., Cybulsky, M. I., Nourshargh, S. Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nature Reviews Immunology. 7, 678-689 (2007).
13. Wilson, E. H., Weninger, W., Hunter, C. A. Trafficking of immune cells in the central nervous system. Journal of Clinical Investigation. 120, 1368-1379 (2010).
14. Engelhardt, B., Capture Ransohoff, R. M. crawl, cross: the T cell code to breach the blood-brain barriers. Trends in Immunology. 33, 579-589 (2012).
15. Zonta, M., et al. Neuron-to-astrocyte signaling is central to the dynamic control of brain microcirculation. Nature Neuroscience. 6, 43-50 (2003).
16. Daneman, R., Zhou, L., Kebede, A. A., Barres, B. A. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature. 468, 562-566 (2010).
17. Gerhardt, H., et al. VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia. Journal of Cell Biology. 161, 1163-1177 (2003).
18. Engelhardt, B., Sorokin, L. The blood-brain and the blood-cerebrospinal fluid barriers: function and dysfunction. Seminars in immunopathology. 31, 497-511 (2009).
19. Perriere, N., et al. Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties. J. Neurochem. 93, 279-289 (2005).
20. Ruck, T., et al. CD4+NKG2D+ T cells exhibit enhanced migratory and encephalitogenic properties in neuroinflammation. PLoS One. 8, 81455(2013).
21. Weidenfeller, C., Schrot, S., Zozulya, A., Galla, H. J. Murine brain capillary endothelial cells exhibit improved barrier properties under the influence of hydrocortisone. Brain Res. 1053, 162-174 (2005).
22. Gumbleton, M., Audus, K. L. Progress and limitations in the use of in vitro cell cultures to serve as a permeability screen for the blood-brain barrier. J. Pharmaceutal Sci. 90, 1681-1698 (2001).
23. Watanabe, T., et al. Paracellular barrier and tight junction protein expression in the immortalized brain endothelial cell lines bEND.3, bEND.5 and mouse brain endothelial cell 4. Biological & Pharmaceutical Bulletin. 36, 492-495 (2013).
24. Omidi, Y., et al. Evaluation of the immortalised mouse brain capillary endothelial cell line, b.End3, as an in vitro blood-brain barrier model for drug uptake and transport studies. Brain Research. 990, 95-112 (2003).
25. Sacharidou, A., et al. Endothelial lumen signaling complexes control 3D matrix-specific tubulogenesis through interdependent Cdc42- and MT1-MMP-mediated events. Blood. 115, 5259-5269 (2010).
26. Urich, E., et al. Multicellular self-assembled spheroidal model of the blood brain barrier. Scientific reports.3, (2013).