In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-carcinoembryonic antigen antibody in two-photon excitation microscopy

Cancer Science

Volumn 105, Issue 10, 2014, Pages 1299-1306

  Koga, Shigehiro ^a,b   Oshima, Yusuke ^a,b,c,d   Honkura, Naoki ^a,b,e   Iimura, Tadahiro ^b,c,d   Kameda, Kenji ^c   Sato, Koichi ^a   Yoshida, Motohira ^a   Yamamoto, Yuji ^a   Watanabe, Yuji ^a   Hikita, Atsuhiko ^a,b,c   Imamura, Takeshi ^a,b,c,d  

^a EHIME UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c EHIME UNIVERSITY   (Japan)

^d EHIME UNIVERSITY HOSPITAL   (Japan)

^e UPPSALA UNIVERSITY   (Sweden)

Author keywords

Cancer cells; Carcinoembryonic antigen; Fluorophore conjugated antibodies; in vivo fluorescence imaging; Two photon excitation microscopy

Indexed keywords

ALEXA594; CARCINOEMBRYONIC ANTIGEN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; ORGANIC COMPOUND;

ANIMAL; BAGG ALBINO MOUSE; DIAGNOSTIC USE; FEMALE; FLUORESCENCE MICROSCOPY; HUMAN; IMMUNOLOGY; LYMPH NODE METASTASIS; MOUSE; NEOPLASMS, EXPERIMENTAL; PROCEDURES; TUMOR CELL LINE;

ANIMALS; CARCINOEMBRYONIC ANTIGEN; CELL LINE, TUMOR; FEMALE; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; LYMPHATIC METASTASIS; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; NEOPLASMS, EXPERIMENTAL; ORGANIC CHEMICALS;

EID: 84911092453     PISSN: 13479032     EISSN: 13497006     Source Type: Journal    
DOI: 10.1111/cas.12500     Document Type: Article

References (27)

1. Chaffer CL, Weinberg RA. A perspective on cancer cell metastasis. Science 2011; 331: 1559-64.
2. Massoud TF, Gambhir SS. Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev 2003; 17: 545-80.
3. Weissleder R, Pittet MJ. Imaging in the era of molecular oncology. Nature 2008; 452: 580-9.
4. Kobayashi H, Longmire MR, Ogawa M, Choyke PL, Kawamoto S. Multiplexed imaging in cancer diagnosis: applications and future advances. Lancet Oncol 2010; 11: 589-95.
5. Withrow KP, Newman JR, Skipper JB et al. Assessment of bevacizumab conjugated to Cy5.5 for detection of head and neck cancer xenografts. Technol Cancer Res Treat 2008; 7: 61-6.
6. Kulbersh BD, Duncan RD, Magnuson JS, Skipper JB, Zinn K, Rosenthal EL. Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Arch Otolaryngol Head Neck Surg 2007; 133: 511-5.
7. Koyama Y, Hama Y, Urano Y, Nguyen DM, Choyke PL, Kobayashi H. Spectral fluorescence molecular imaging of lung metastases targeting HER2/neu. Clin Cancer Res 2007; 13: 2936-45.
8. Hama Y, Urano Y, Koyama Y, Choyke PL, Kobayashi H. Activatable fluorescent molecular imaging of peritoneal metastases following pretargeting with a biotinylated monoclonal antibody. Cancer Res 2007; 67: 3809-17.
9. McElroy M, Kaushal S, Luiken GA et al. Imaging of primary and metastatic pancreatic cancer using a fluorophore-conjugated anti-CA19-9 antibody for surgical navigation. World J Surg 2008; 32: 1057-66.
10. Cooper EH, Turner R, Steele L, Neville AM, Mackay AM. The contribution of serum enzymes and carcinoembryonic antigen to the early diagnosis of metastatic colorectal cancer. Br J Cancer 1975; 31: 111-7.
11. Wolf B, Thompson J, Von Kleist S. Ultrastructural localization of carcino-embryonic antigen in a continuous human tumour cell line (LS 174 T) in relation to morphometric investigations. Anticancer Res 1984; 4: 213-9.
12. Goldstein MJ, Mitchell EP. Carcinoembryonic antigen in the staging and follow-up of patients with colorectal cancer. Cancer Invest 2005; 23: 338-51.
13. Ito S, Muguruma N, Kusaka Y et al. Detection of human gastric cancer in resected specimens using a novel infrared fluorescent anti-human carcinoembryonic antigen antibody with an infrared fluorescence endoscope in vitro. Endoscopy 2001; 33: 849-53.
14. Lisy MR, Goermar A, Thomas C et al. In vivo near-infrared fluorescence imaging of carcinoembryonic antigen-expressing tumor cells in mice. Radiology 2008; 247: 779-87.
15. 3 regenerative amplifier. Opt Lett 2003; 28: 1022-4.
16. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J. High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harb Protoc 2011; 2011: 1167-84.
17. Hanyu A, Kojima K, Hatake K et al. Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells. Cancer Sci 2009; 100: 2085-92.
18. Ehata S, Hanyu A, Fujime M et al. Ki26894, a novel transforming growth factor-beta type I receptor kinase inhibitor, inhibits in vitro invasion and in vivo bone metastasis of a human breast cancer cell line. Cancer Sci 2007; 98: 127-33.
19. Goldman LA, Cutrone EC, Kotenko SV, Krause CD, Langer JA. Modifications of vectors pEF-BOS, pcDNA1 and pcDNA3 result in improved convenience and expression. Biotechniques 1996; 21: 1013-5.
20. Katayama K, Wada K, Miyoshi H et al. RNA interfering approach for clarifying the PPARgamma pathway using lentiviral vector expressing short hairpin RNA. FEBS Lett 2004; 560: 178-82.
21. Hayashi K, Jiang P, Yamauchi K et al. Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res 2007; 67: 8223-8.
22. Yang M, Baranov E, Wang JW et al. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci U S A 2002; 99: 3824-9.
23. Chen H, Wang H, Slipchenko MN et al. A multimodal platform for nonlinear optical microscopy and microspectroscopy. Opt Express 2009; 17: 1282-90.
24. Hilger I, Leistner Y, Berndt A et al. Near-infrared fluorescence imaging of HER-2 protein over-expression in tumour cells. Eur Radiol 2004; 14: 1124-9.
25. Petrovsky A, Schellenberger E, Josephson L, Weissleder R, Bogdanov A Jr. Near-infrared fluorescent imaging of tumor apoptosis. Cancer Res 2003; 63: 1936-42.
26. Urano Y, Asanuma D, Hama Y et al. Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes. Nat Med 2009; 15: 104-9.
27. Centonze VE, White JG. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J 1998; 75: 2015-24.