Comparing whole-genome sequencing with sanger sequencing for spa typing of methicillin-resistant staphylococcus aureus

Journal of Clinical Microbiology

Volumn 52, Issue 12, 2014, Pages 4305-4308

  Bartels, Mette Damkjær ^a   Petersen, Andreas ^b   Worning, Peder ^a   Nielsen, Jesper Boye ^a   Larner Svensson, Hanna ^a,b   Johansen, Helle Krogh ^a   Andersen, Leif Percival ^a   Jarløv, Jens Otto ^a   Boye, Kit ^a   Larsen, Anders Rhod ^b   Westh, Henrik ^a,c  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b STATENS SERUM INSTITUT   (Denmark)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM ISOLATE; DENMARK; GENE SEQUENCE; GENETIC PROCEDURES; INTERMETHOD COMPARISON; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS INFECTION; NONHUMAN; POLYMERASE CHAIN REACTION; RELIABILITY; SANGER SEQUENCING; SINGLE NUCLEOTIDE POLYMORPHISM; SPA TYPING; WHOLE GENOME SEQUENCING; CLASSIFICATION; COMPARATIVE STUDY; DNA SEQUENCE; EVALUATION STUDY; GENETICS; HUMAN; MICROBIOLOGY; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; PROCEDURES; STAPHYLOCOCCAL INFECTIONS;

STAPHYLOCOCCUS PROTEIN A;

DENMARK; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCAL PROTEIN A;

EID: 84910145816     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01979-14     Document Type: Article

References (10)

1. Grundmann H, Aires-de-Sousa M, Boyce J, Tiemersma E. 2006. Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368:874-885. http://dx.doi.org/10.1016/S0140-6736(06)68853-3.
2. Otto M. 2013. Community-associated MRSA: what makes them special? Int. J. Med. Microbiol. 303:324-330. http://dx.doi.org/10.1016/j.ijmm.2013.02.007.
3. Harmsen D, Claus H, Witte W, Rothganger J, Claus H, Turnwald D, Vogel U. 2003. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41:5442-5448. http://dx.doi.org/10.1128/JCM.41.12.5442-5448.2003.
4. Aires-de-Sousa M, Boye K, de Lencastre H, Deplano A, Enright MC, Etienne J, Friedrich A, Harmsen D, Holmes A, Huijsdens XW, Kearns AM, Mellmann A, Meugnier H, Rasheed JK, Spalburg E, Strommenger B, Struelens MJ, Tenover FC, Thomas J, Vogel U, Westh H, Xu J, Witte W. 2006. High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study. J. Clin. Microbiol. 44:619-621. http://dx.doi.org/10.1128/JCM.44.2.619-621.2006.
5. Didelot X, Bowden R, Wilson DJ, Peto TE, Crook DW. 2012. Transforming clinical microbiology with bacterial genome sequencing. Nat. Rev. Genet. 13:601-612. http://dx.doi.org/10.1038/nrg3226.
6. Eyre DW, Golubchik T, Gordon NC, Bowden R, Piazza P, Batty EM, Ip CL, Wilson DJ, Didelot X, O'Connor L, Lay R, Buck D, Kearns AM, Shaw A, Paul J, Wilcox MH, Donnelly PJ, Peto TE, Walker AS, Crook DW. 2012. A pilot study of rapid benchtop sequencing of Staphylococcus aureus and Clostridium difficile for outbreak detection and surveillance. BMJ Open 2:e001124. http://dx.doi.org/10.1136/bmjopen-2012-001124.
7. Stegger M, Andersen PS, Kearns A, Pichon B, Holmes MA, Edwards G, Laurent F, Teale C, Skov R, Larsen AR. 2012. Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251). Clin. Microbiol. Infect. 18:395-400. http://dx.doi.org/10.1111/j.1469-0691.2011.03715.x.
8. Salzberg SL, Yorke JA. 2005. Beware of mis-assembled genomes. Bioinformatics 21:4320-4321. http://dx.doi.org/10.1093/bioinformatics/bti769.
9. Mellmann A, Weniger T, Berssenbrugge C, Rothganger J, Sammeth M, Stoye J, Harmsen D. 2007. Based Upon Repeat Pattern (BURP): an algorithm to characterize the long-term evolution of Staphylococcus aureus populations based on spa polymorphisms. BMC Microbiol. 7:98. http://dx.doi.org/10.1186/1471-2180-7-98.
10. Leopold SR, Goering RV, Witten A, Harmsen D, Mellmann A. 2014. Bacterial whole-genome sequencing revisited: portable, scalable, and standardized analysis for typing and detection of virulence and antibiotic resistance genes. J. Clin. Microbiol. 52:2365-2370. http://dx.doi.org/10.1128/JCM.00262-14.