Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecALGA251

Clinical Microbiology and Infection

Volumn 18, Issue 4, 2012, Pages 395-400

  Stegger, M ^a   Andersen, P S ^a   Kearns, A ^b   Pichon, B ^b   Holmes, M A ^c   Edwards, G ^d   Laurent, F ^e   Teale, C ^f   Skov, R ^a   Larsen, A R ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d Scottish MRSA Reference Laboratory   (United Kingdom)

^e Centre National de Reference des Staphylocoques   (France)

^f VETERINARY LABORATORIES AGENCY   (United Kingdom)

Author keywords

MecA; MecC; Methicillin resistant Staphylococcus aureus; Panton Valentine leukocidin; SCCmec; Staphylococcus aureus

Indexed keywords

PENICILLIN BINDING PROTEIN 2A;

AMPLICON; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; GENE SEQUENCE; GENETIC DIFFERENCE; GENETIC IDENTIFICATION; GENETIC VARIABILITY; GENOTYPE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SENSITIVE STAPHYLOCOCCUS AUREUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VALIDATION PROCESS;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 84858679784     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2011.03715.x     Document Type: Article

References (13)

1. Garcia-Alvarez L, Holden MTG, Lindsay H et al. Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis 2011; 11: 595-603.
2. Shore AC, Deasy EC, Slickers P et al. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2011; 55: 3765-3773.
3. Fluit AC. What to do with MRSA with a novel mec gene? Lancet Infect Dis 2011; 11: 580-581.
4. Cuny C, Layer F, Strommenger B, Witte W. Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PLoS One 2011; 6: e24360. Sep 8 [Epub ahead of print].
5. Larsen AR, Stegger M, Sorum M. spa typing directly from a mecA, spa and pvl multiplex PCR assay-a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance. Clin Microbiol Infect 2008; 14: 611-614.
6. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 2000; 38: 1008-1015.
7. DANMAP. 2010. Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark. ISSN: 1600-2032.
8. Annual Report on Staphylococcus aureus bacteraemia cases in Denmark 2009.
9. Kondo Y, Ito T, Ma XX et al. Combination of multiplex PCRs for SCCmec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 2007; 51: 264-274.
10. Chambers HF. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 1997; 10: 781-791.
11. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2002; 46: 2155-2161.
12. Harmsen D, Claus H, Witte W et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003; 41: 5442-5448.
13. Deurenberg RH, Vink C, Driessen C et al. Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR. FEMS Microbiol Lett 2004; 240: 225-228.