Electrochemical detection of protein kinase activity based on carboxypeptidase Y digestion triggered signal amplification

Biosensors and Bioelectronics

Volumn 66, Issue , 2015, Pages 77-83

  Yin, Huanshun ^a   Wang, Xinxu ^a   Guo, Yunlong ^a   Zhou, Yunlei ^a   Ai, Shiyun ^a  

^a SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Carboxypeptidase Y; Electrochemical biosensor; Phosphorylation; Protein kinase

Indexed keywords

BIOSENSORS; DIAGNOSIS; ENZYMES; PEPTIDES; PHOSPHORYLATION; PROTEINS; SIGNAL DETECTION; SIGNAL PROCESSING;

CARBOXYPEPTIDASE; ELECTROCHEMICAL BIOSENSOR; ELECTROCHEMICAL DETECTION; ELECTROCHEMICAL METHODS; ELECTROCHEMICAL SIGNALS; INHIBITOR CONCENTRATION; PROTEIN KINASE; PROTEIN KINASE ACTIVITIES;

CHEMICAL DETECTION;

ASPARTIC ACID; CARBOXYPEPTIDASE C; CASEIN KINASE II; ELLAGIC ACID; PEPTIDE; PROTEIN KINASE INHIBITOR;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; ELECTROCHEMICAL ANALYSIS; ELECTRODE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; ENZYMIC BIOSENSOR; HUMAN; HUMAN CELL; HYDROLYSIS; IC50; LIMIT OF DETECTION; OXIDATION REDUCTION POTENTIAL; SIGNAL AMPLIFICATION; SIGNAL PROCESSING; AMINO ACID SEQUENCE; ANTAGONISTS AND INHIBITORS; CHEMISTRY; DRUG SCREENING; GENETIC PROCEDURES; HEPG2 CELL LINE; METABOLISM; PHOSPHORYLATION; PROCEDURES;

AMINO ACID SEQUENCE; BIOSENSING TECHNIQUES; CASEIN KINASE II; CATHEPSIN A; DRUG EVALUATION, PRECLINICAL; ELECTROCHEMICAL TECHNIQUES; ENZYME ASSAYS; HEP G2 CELLS; HUMANS; LIMIT OF DETECTION; PEPTIDES; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS;

EID: 84910131159     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2014.11.014     Document Type: Article

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