Construction of dengue virus protease expression plasmid and in vitro protease assay for screening antiviral inhibitors

Methods in Molecular Biology

Volumn 1138, Issue , 2014, Pages 345-360

  Lai, Huiguo ^a   Teramoto, Tadahisa ^a   Padmanabhan, Radhakrishnan ^a  

^a NONE

Author keywords

Aprotinin; Dengue virus serotype 2; Fluorogenic peptide substrate; In vitro protease assay; NS2B cofactor; NS3 protease domain; Trypsin inhibitor

Indexed keywords

ANTIVIRUS AGENT; PROTEINASE; NS3 PROTEIN, FLAVIVIRUS; PROTEINASE INHIBITOR; RNA HELICASE; SERINE PROTEINASE; VIRUS PROTEIN;

ARTICLE; DENGUE VIRUS 2; DRUG DETERMINATION; DRUG SCREENING; IN VITRO STUDY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; DENGUE VIRUS; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; HUMAN; IC 50; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR LIBRARY; TEMPERATURE;

DENGUE VIRUS; MIRIDAE;

ANTIVIRAL AGENTS; DENGUE VIRUS; DRUG EVALUATION, PRECLINICAL; ENZYME ASSAYS; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; PLASMIDS; PROTEASE INHIBITORS; RNA HELICASES; SERINE ENDOPEPTIDASES; SMALL MOLECULE LIBRARIES; SUBSTRATE SPECIFICITY; TEMPERATURE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84910120725     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0348-1_21     Document Type: Article

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