Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India

Journal of Virological Methods

Volumn 211, Issue , 2015, Pages 22-31

  Neeraja, M ^a   Lakshmi, V ^a   Lavanya, Vanjari ^a   Priyanka, E N ^a   Parida, M M ^b   Dash, P K ^b   Sharma, Shashi ^b   Rao, P V Lakshmana ^d   Reddy, Gopal ^c  

^a NIZAM'S INSTITUTE OF MEDICAL SCIENCES   (India)

^b DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^c OSMANIA UNIVERSITY   (India)

^d DRDO BU Center for Life Sciences   (India)

Author keywords

DENV serotypes; Genie II flourometer; NS 1 antigen; RT LAMP; RT PCR; Serotyping

Indexed keywords

ANTIGEN; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; NONSTRUCTURAL PROTEIN 1; NONSTRUCTURAL PROTEIN 1 ANTIGEN; UNCLASSIFIED DRUG; VIRUS RNA; VIRUS PROTEIN;

ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; DENGUE; DENGUE VIRUS; DENGUE VIRUS 1; DENGUE VIRUS 2; DENGUE VIRUS 3; DENGUE VIRUS 4; DIAGNOSTIC TEST ACCURACY STUDY; DISEASE SURVEILLANCE; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUOROMETER; HUMAN; INDIA; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MEASUREMENT REPEATABILITY; NONHUMAN; NS1 GENE; NUCLEOTIDE SEQUENCE; PHYLOGENY; PREDICTIVE VALUE; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; STANDARDIZATION; TERTIARY CARE CENTER; VALIDATION PROCESS; VIRUS DETECTION; CLASSIFICATION; COMPARATIVE STUDY; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; PROCEDURES; REVERSE TRANSCRIPTION; SEROTYPE; STANDARDS; TIME; VALIDATION STUDY; VIROLOGY;

DENGUE; DENGUE VIRUS; HUMANS; INDIA; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REVERSE TRANSCRIPTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SEROGROUP; TERTIARY CARE CENTERS; TIME FACTORS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84910051718     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.10.005     Document Type: Article

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