Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii

Journal of Biological Chemistry

Volumn 289, Issue 45, 2014, Pages 31382-31396

  Buchinger, Edith ^a,b   Knudsen, Daniel H ^a   Behrens, Manja A ^c   Pedersen, Jan Skov ^c   Aarstad, Olav A ^b   Tøndervik, Anne ^d   Valla, Svein ^c   Skjåk Bræk, Gudmund ^b   Wimmer, Reinhard ^a   Aachmann, Finn L ^b  

^a AALBORG UNIVERSITY   (Denmark)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^c AARHUS UNIVERSITY   (Denmark)

^d SINTEF MATERIALS AND CHEMISTRY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINATE; OLIGOMERS; TITRATION;

DEGREE OF FLEXIBILITY; FUNCTIONAL CHARACTERIZATION; ISOTHERMAL TITRATION CALORIMETRY; MANNURONAN C-5 EPIMERASES; MODULAR STRUCTURES; POSITIVELY CHARGED; STRONG INTERACTION; WEAK INTERACTIONS;

X RAY SCATTERING;

ALGINATE EPIMERASE 4; ALGINATE EPIMERASE 6; ALGINIC ACID; CALCIUM ION; CARBOXYLIC ACID; EPIMERASE; GULURONIC ACID; MANNURONIC ACID; OLIGOMER; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CALCIUM BINDING PROTEIN; GLUCURONIC ACID; HEXURONIC ACID;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL STRAIN; BINDING AFFINITY; ENZYME BINDING; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; EPIMERIZATION; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLASMID; PROTEIN FOLDING; R MODULE; SEQUENCE ALIGNMENT; STRUCTURE ACTIVITY RELATION; SURFACE PROPERTY; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; BINDING SITE; CALORIMETRY; CATALYSIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; MOLECULAR GENETICS; PROTEIN ENGINEERING; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; RADIATION SCATTERING; SEQUENCE HOMOLOGY; X RAY;

ALGINATES; AMINO ACID SEQUENCE; AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; BINDING SITES; CALCIUM-BINDING PROTEINS; CALORIMETRY; CARBOHYDRATE EPIMERASES; CATALYSIS; ESCHERICHIA COLI; GLUCURONIC ACID; HEXURONIC ACIDS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SCATTERING, RADIATION; SEQUENCE HOMOLOGY, AMINO ACID; X-RAYS;

EID: 84909944344     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.567008     Document Type: Article

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