Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot

Human Vaccines and Immunotherapeutics

Volumn 10, Issue 8, 2014, Pages 2490-2496

  Trück, Johannes ^a   Mitchell, Ruth ^a   Thompson, Amber J ^a   Morales Aza, Begonia ^b   Clutterbuck, Elizabeth A ^a   Kelly, Dominic F ^a   Finn, Adam ^b   Pollard, Andrew J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

B cells; Cryopreservation; ELISpot; Memory B cells; PBMC; Shipment; Storage; Vaccination

Indexed keywords

DIPHTHERIA TETANUS TOXOID; BACTERIAL ANTIGEN;

ANTIGEN DETECTION; ARTICLE; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYOPRESERVATION; ELISA KIT; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; HUMAN CELL; MEMORY CELL; OBSERVER VARIATION; PERIPHERAL BLOOD MONONUCLEAR CELL; PROSPECTIVE STUDY; REPRODUCIBILITY; B LYMPHOCYTE; EVALUATION STUDY; IMMUNOLOGICAL MEMORY; IMMUNOLOGY; LABORATORY DIAGNOSIS; LEUKOCYTE COUNT; MONONUCLEAR CELL; NORMAL HUMAN; PROCEDURES;

ANTIGENS, BACTERIAL; B-LYMPHOCYTES; CRYOPRESERVATION; ENZYME-LINKED IMMUNOSPOT ASSAY; HEALTHY VOLUNTEERS; HUMANS; IMMUNOLOGIC MEMORY; LEUKOCYTE COUNT; LEUKOCYTES, MONONUCLEAR; PROSPECTIVE STUDIES; REPRODUCIBILITY OF RESULTS; SPECIMEN HANDLING;

EID: 84908360086     PISSN: 21645515     EISSN: 2164554X     Source Type: Journal    
DOI: 10.4161/hv.29318     Document Type: Article

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