Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses

Journal of Immunological Methods

Volumn 391, Issue 1-2, 2013, Pages 50-59

  Jahnmatz, Maja ^a   Kesa, Gun ^b   Netterlid, Eva ^a   Buisman, Anne Marie ^c   Thorstensson, Rigmor ^a   Ahlborg, Niklas ^b  

^a SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^b Mabtech AB ^*  (Sweden)

^c NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

Author keywords

B cell ELISpot; Biotinylation; Monoclonal antibody; Optimization; R848

Indexed keywords

ANTIGEN; AVIDIN; BIOTIN; CPG OLIGODEOXYNUCLEOTIDE; DIPHTHERIA PERTUSSIS TETANUS VACCINE; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; INTERLEUKIN 10; INTERLEUKIN 2; RECOMBINANT INTERLEUKIN 2; RESIQUIMOD; VACCINE;

ARTICLE; B LYMPHOCYTE; CELLULAR IMMUNITY; CLINICAL PROTOCOL; CONTROLLED STUDY; DIPHTHERIA; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; MEMORY CELL; NUCLEIC ACID AMPLIFICATION; PERIPHERAL BLOOD MONONUCLEAR CELL; PERTUSSIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; TETANUS; VACCINATION;

ADOLESCENT; ADULT; ANTIBODIES, ANTI-IDIOTYPIC; ANTIBODIES, MONOCLONAL; B-LYMPHOCYTES; BIOTINYLATION; CELLS, CULTURED; CPG ISLANDS; DIPHTHERIA-TETANUS-ACELLULAR PERTUSSIS VACCINES; ENZYME-LINKED IMMUNOSPOT ASSAY; HUMANS; IMIDAZOLES; IMMUNOGLOBULIN G; IMMUNOLOGIC MEMORY; INTERLEUKIN-10; INTERLEUKIN-2; LYMPHOCYTE ACTIVATION; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TOLL-LIKE RECEPTORS;

EID: 84876118696     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.02.009     Document Type: Article

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