Preparation of synaptic plasma membrane and postsynaptic density proteins using a discontinuous sucrose gradient

Journal of Visualized Experiments

Volumn , Issue 91, 2014, Pages

  Bermejo, Marie Kristel ^a   Milenkovic, Marija ^a   Salahpour, Ali ^a   Ramsey, Amy J ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Brain; Issue 91; Neurobiology; PSD; SPM; Synapse; Ultracentrifugation; Western blot

Indexed keywords

MEMBRANE PROTEIN; NERVE PROTEIN; POSTSYNAPTIC DENSITY PROTEINS; SUCROSE;

ANIMAL; CHEMISTRY; DENSITY GRADIENT CENTRIFUGATION; METABOLISM; MOUSE; PROCEDURES; SYNAPTIC MEMBRANE; SYNAPTOSOME;

ANIMALS; CENTRIFUGATION, DENSITY GRADIENT; MEMBRANE PROTEINS; MICE; NERVE TISSUE PROTEINS; SUCROSE; SYNAPTIC MEMBRANES; SYNAPTOSOMES;

EID: 84908293089     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51896     Document Type: Article

References (38)

1. Delint-Ramirez, I. et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience. 30, 8162-8170 doi:10.1523/JNEUROSCI.1792-10.2010 (2010).
2. Wang, Q. et al. The psychiatric disease risk factors DISC1 and TNIK interact to regulate synapse composition and function. Mol Psychiatry 16 doi:mp201087 [pii] 10.1038/mp.2010.87 (2010).
3. Ehlers, M. D. Reinsertion or degradation of AMPA receptors determined by activity-dependent endocytic sorting. Neuron. 28, 511-525 (2000).
4. Tomita, S., Fukata, M., Nicoll, R. A., & Bredt, D. S. Dynamic interaction of stargazin-like TARPs with cycling AMPA receptors at synapses. Science. 303, 1508-1511 doi:10.1126/science.1090262 (2004).
5. Ehlers, M. D., Heine, M., Groc, L., Lee, M. C., & Choquet, D. Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity. Neuron. 54, 447-460 doi:10.1016/j.neuron.2007.04.010 (2007).
6. Bats, C., Groc, L., & Choquet, D. The interaction between Stargazin and PSD-95 regulates AMPA receptor surface trafficking. Neuron. 53, 719-734 doi:10.1016/j.neuron.2007.01.030 (2007).
7. Ehlers, M. D. Activity level controls postsynaptic composition and signaling via the ubiquitin-proteasome system. Nat Neurosci. 6, 231-242 doi:10.1038/nn1013 nn1013 [pii] (2003).
8. Cartier, E. A. et al. A biochemical and functional protein complex involving dopamine synthesis and transport into synaptic vesicles. J Biol Chem. 285, 1957-1966 doi:10.1074/jbc.M109.054510 M109.054510 [pii] (2010).
9. Rodriguez de Lores, A., Alberici, M., & De Robertis, E. Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex. Journal of Neurochemistry. 14, 215-225 (1967).
10. Gray, E. G., & Whittaker, V. P. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat. 96, 79-88 (1962).
11. Cotman, C. W., & Taylor, D. Isolation and structural studies on synaptic complexes from rat brain. J Cell Biol. 55, 696-711 (1972).
12. Cotman, C. W., Banker, G., Churchill, L., & Taylor, D. Isolation of postsynaptic densities from rat brain. The Journal of Cell Biology. 63, 441-455 (1974).
13. Carlin, R. K., Grab, D. J., Cohen, R. S., & Siekevitz, P. Isolation and characterization of postsynaptic densities from various brain regions: enrichment of different types of postsynaptic densities. The Journal of Cell Biology. 86, 831-845 (1980).
14. Jones, D. H., & Matus, A. I. Isolation of synaptic plasma membrane from brain by combined flotation-sedimentation density gradient centrifugation. Biochim Biophys Acta. 356, 276-287 (1974).
15. Kennedy, M. B., Bennett, M. K., & Erondu, N. E. Biochemical and immunochemical evidence that the 'major postsynaptic density protein' is a subunit of a calmodulin-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America. 80, 7357-7361 (1983).
16. Moon, I. S., Apperson, M. L., & Kennedy, M. B. The major tyrosine-phosphorylated protein in the postsynaptic density fraction is N-methyl-D-aspartate receptor subunit 2B. Proceedings of the National Academy of Sciences of the United States of America. 91, 3954-3958 (1994).
17. Cho, K. O., Hunt, C. A., & Kennedy, M. B. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron. 9, 929-942 (1992).
18. Salahpour, A. et al. Increased amphetamine-induced hyperactivity and reward in mice overexpressing the dopamine transporter. Proc Natl Acad Sci U S A. 105, 4405-4410 doi:0707646105 [pii] 10.1073/pnas.0707646105 (2008).
19. Ma, D. K. et al. Neuronal activity-induced Gadd45b promotes epigenetic DNA demethylation and adult neurogenesis. Science. 323, 1074-1077 doi:10.1126/science.1166859 1166859 [pii] (2009).
20. Budreck, E. C., & Scheiffele, P. Neuroligin-3 is a neuronal adhesion protein at GABAergic and glutamatergic synapses. The European Journal of Neuroscience. 26, 1738-1748 doi:10.1111/j.1460-9568.2007.05842.x (2007).
21. Wu, J. et al. Arc/Arg3.1 regulates an endosomal pathway essential for activity-dependent beta-amyloid generation. Cell. 147, 615-628 doi:10.1016/j.cell.2011.09.036 (2011).
22. Huttner, W. B., Schiebler, W., Greengard, P., & De Camilli, P. Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. J Cell Biol. 96, 1374-1388 (1983).
23. Nagy, A., Baker, R. R., Morris, S. J., & Whittaker, V. P. The preparation and characterization of synaptic vesicles of high purity. Brain Res. 109, 285-309 doi:0006-8993(76)90531-X [pii] (1976).
24. Cheng, D. et al. Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum. Molecular & Cellular Proteomics: MCP. 5, 1158-1170 doi:10.1074/mcp.D500009-MCP200 (2006).
25. Sheng, M., & Hoogenraad, C. C. The postsynaptic architecture of excitatory synapses: a more quantitative view. Annu Rev Biochem. 76, 823-847 doi:10.1146/annurev.biochem.76.060805.160029 (2007).
26. Sambrook, J., Fritsch, E. F., & Maniatis, T. Molecular cloning: a Laboratory Manual. 2nd edn, Cold Spring Harbor Laboratory Press, (1989).
27. Eggena, M. et al. Identification of histone H1 as a cognate antigen of the ulcerative colitis-associated marker antibody pANCA. J Autoimmun. 14, 83-97 doi:10.1006/jaut.1999.0340 S0896-8411(99)90340-2 [pii] (2000).
28. Salahpour, A. et al. Homodimerization of the beta2-adrenergic receptor as a prerequisite for cell surface targeting. J Biol Chem. 279, 33390-33397 doi:10.1074/jbc.M403363200 M403363200 [pii] (2004).
29. Bernocco, S. et al. Sequential detergent fractionation of primary neurons for proteomics studies. Proteomics. 8, 930-938 doi:10.1002/pmic.200700738 (2008).
30. Grunewald, T. G. et al. Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma. BMC Cancer. 7, 198 doi:1471-2407-7-198 [pii] 10.1186/1471-2407-7-198 (2007).
31. Heimann, K., Percival, J. M., Weinberger, R., Gunning, P., & Stow, J. L. Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles. J Biol Chem. 274, 10743-10750 (1999).
32. Neff, R. A.,, Gomez-Varela, D., Fernandes, C. C., & Berg, D. K. Postsynaptic scaffolds for nicotinic receptors on neurons. Acta Pharmacol Sin. 30, 694-701 doi:10.1038/aps.2009.52 aps200952 [pii] (2009).
33. Cella, N., Cornejo-Uribe, R. R., Montes, G. S., Hynes, N. E., & Chammas, R. The lysosomal-associated membrane protein LAMP-1 is a novel differentiation marker for HC11 mouse mammary epithelial cells. Differentiation. 61, 113-120 doi:S0301-4681(09)60753-2 [pii] 10.1046/j.1432-0436.1996.6120113.x (1996).
34. Otera, H. et al. Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import. Mol Cell Biol. 22, 1639-1655 (2002).
35. Goubaeva, F. et al. Cardiac mitochondrial connexin 43 regulates apoptosis. Biochem Biophys Res Commun. 352, 97-103 doi:S0006-291X(06)02417-X [pii] 10.1016/j.bbrc.2006.10.177 (2007).
36. Lamers, K. J. et al. Protein S-100B, neuron-specific enolase (NSE), myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) in cerebrospinal fluid (CSF) and blood of neurological patients. Brain Res Bull. 61, 261-264 doi:S0361923003000893 [pii] (2003).
37. Arunachalam, L. et al. Munc18-1 is critical for plasma membrane localization of syntaxin1 but not of SNAP-25 in PC12 cells. Mol Biol Cell. 19, 722-734 doi:E07-07-0662 [pii] 10.1091/mbc.E07-07-0662 (2008).
38. Brandstatter, J. H., Fletcher, E. L., Garner, C. C., Gundelfinger, E. D., & Wassle, H. Differential expression of the presynaptic cytomatrix protein bassoon among ribbon synapses in the mammalian retina. Eur J Neurosci. 11, 3683-3693 doi:ejn793 [pii] (1999).