Multi-residue analysis of emerging pollutants in benthic invertebrates by modified micro-quick-easy-cheap-efficient-rugged-safe extraction and nanoliquid chromatography-nanospray-tandem mass spectrometry analysis

Journal of Chromatography A

Volumn 1367, Issue , 2014, Pages 16-32

  Berlioz Barbier, Alexandra ^a   Buleté, Audrey ^a   Faburé, Juliette ^b   Garric, Jeanne ^b   Cren Olivé, Cécile ^a   Vulliet, Emmanuelle ^a  

^a UNIVERSITÉ LYON 1   (France)

^b CEMAGREF   (France)

Author keywords

Benthic invertebrates; Emerging pollutants; MS MS; Multi residue method; Nano LC; Sample preparation

Indexed keywords

AGRICULTURAL ROBOTS; ANIMALS; AQUATIC ECOSYSTEMS; BIOACCUMULATION; BIOCHEMISTRY; DRUG PRODUCTS; EFFLUENTS; EXTRACTION; IONIZATION OF LIQUIDS; LIQUIDS; MASS SPECTROMETRY; POLLUTION; SAMPLING;

BENTHIC INVERTEBRATES; EMERGING POLLUTANTS; MS/MS; MULTIRESIDUE METHOD; NANO-LC; SAMPLE PREPARATION;

LIQUID CHROMATOGRAPHY;

17ALPHA ESTRADIOL; 4,4' ISOPROPYLIDENEDIPHENOL; AFIMOXIFENE; AMITRIPTYLINE; ATENOLOL; BEZAFIBRATE; CARBAMAZEPINE; CYCLOPHOSPHAMIDE; DESLORATADINE; DICLOFENAC; DIURON; ECONAZOLE; ESTRADIOL; ESTRONE; ETHINYLESTRADIOL; IBUPROFEN; KETOPROFEN; LIDOCAINE; MIFEPRISTONE; NICARDIPINE; NORETHISTERONE; OXAZEPAM; PANTOPRAZOLE; PREDNISOLONE; RITONAVIR; ROXITHROMYCIN; SPINOSAD; TAMOXIFEN; TESTOSTERONE; NANOMATERIAL; POLLUTANT;

AQUATIC ENVIRONMENT; ARTICLE; BENTHOS; BIOACCUMULATION; CHIRONOMUS RIPARIUS; CONCENTRATION (PARAMETERS); ECOTOXICOLOGY; ELECTROSPRAY; FRESHWATER SPECIES; GAMMARUS FOSSARUM; INTRASPECIFIC VARIATION; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MICRO QUICK EASY CHEAP EFFICIENT RUGGED SAFE EXTRACTION; NANOELECTROSPRAY IONIZATION; NANOLIQUID CHROMATOGRAPHY; NONHUMAN; POTAMOPYRGUS ANTIPODARUM; PROCESS OPTIMIZATION; PURIFICATION; RESIDUE ANALYSIS; SAMPLE SIZE; SPECIES DIFFERENCE; TANDEM MASS SPECTROMETRY; VALIDATION PROCESS; WATER POLLUTANT; AMPHIPODA; ANALYSIS; ANIMAL; CHEMISTRY; CHIRONOMIDAE; ECONOMICS; GASTROPOD; POLLUTANT; PROCEDURES;

AMPHIPODA; ANIMALS; CHIRONOMIDAE; CHROMATOGRAPHY, LIQUID; ENVIRONMENTAL POLLUTANTS; GASTROPODA; LIQUID-LIQUID EXTRACTION; NANOSTRUCTURES; TANDEM MASS SPECTROMETRY;

EID: 84908220869     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.09.044     Document Type: Article

References (92)

1. Jiang J.-Q., Zhou Z., Sharma V.K. Occurrence, transportation, monitoring and treatment of emerging micro-pollutants in waste water - a review from global views. Microchem. J. 2013, 110:292-300.
2. Calderon-Preciado D., Matamoros V., Bayona J.M. Occurrence and potential crop uptake of emerging contaminants and related compounds in an agricultural irrigation network. Sci. Total Environ. 2011, 412:14-19.
3. Brack W., Altenburger R., Schüürmann G., Krauss M., López Herráez D., van Gils J., Slobodnik J., Munthe J., Gawlik B.M., van Wezel A., Schriks M., Hollender J., Tollefsen K.E., Mekenyan O., Dimitrov S., Bunke D., Cousins I., Posthuma L., van den Brink P.J., López de Alda M., Barceló D., Faust M., Kortenkamp A., Scrimshaw M., Ignatova S., Engelen G., Massmann G., Lemkine G., Teodorovic I., Walz K.-H., Dulio V., Jonker M.T.O., Jäger F., Chipman K., Falciani F., Liska I., Rooke D., Zhang X., Hollert H., Vrana B., Hilscherova K., Kramer K., Neumann S., Hammerbacher R., Backhaus T., Mack J., Segner H., Escher B., de Aragão Umbuzeiro G. The SOLUTIONS project: challenges and responses for present and future emerging pollutants in land and water resources management. Sci. Total Environ. 2014, in press. 10.1016/j.scitotenv.2014.05.143.
4. Li L., Zheng B., Liu L. Biomonitoring and bioindicators used for river ecosystems: definitions, approaches and trends. Procedia Environ. Sci. 2010, 2:1510-1524.
5. Zuykov M., Pelletier E., Harper D.A.T. Bivalve mollusks in metal pollution studies: from bioaccumulation to biomonitoring. Chemosphere 2013, 93:201-208.
6. Rosenberg D.M., Resh V.H. Freshwater Biomonitoring and Benthic Macroinvertebrates 1993, Chapman and Hall, New York.
7. Rittschof D., McClellan-Green P. Molluscs as multidisciplinary models in environment toxicology. Mar. Pollut. Bull. 2005, 50:369-373.
8. Khamis K., Hannah D.M., Brown L.E., Tiberti R., Milner A.M. The use of invertebrates as indicators of environmental change in alpine rivers and lakes. Sci. Total Environ. 2014, 493:1242-1254.
9. Maenpaa K.A., Sormunen A.J., Kukkonen J.V.K. Bioaccumulation and toxicity of sediment associated herbicides (ioxynil, pendimethalin, and bentazone) in Lumbriculus variegatus (Oligochaeta) and Chironomus riparius (Insecta). Ecotoxicol. Environ. Saf. 2003, 56:398-410.
10. Besse J.-P., Coquery M., Lopes C., Chaumot A., Budzinski H., Labadie P., Geffard O. Caged Gammarus fossarum (Crustacea) as a robust tool for the characterization of bioavailable contamination levels in continental waters: towards the determination of threshold values. Water Res. 2013, 47:650-660.
11. Castro B.B., Guilhermino U., Ribeiro R. In situ bioassay chambers and procedures for assessment of sediment toxicity with Chironomus riparius. Environ. Pollut. 2003, 125:325-335.
12. Gust M., Buronfosse T., Geffard O., Mons R., Queau H., Mouthon J., Garric J. In situ biomonitoring of freshwater quality using the New-Zealand mudsnail Potamopyrgus antipodarum (Gray) exposed to waste water treatment plant (WWTP) effluent discharges. Water Res. 2010, 44:4517-4528.
13. Schmitt C., Vogt C., Van Ballaer B., Brix R., Suetens A., Schmitt-Jansen M., De Deckere E. In situ cage experiments with Potamopyrgus antipodarum - a novel tool for real life exposure assessment in freshwater ecosystems. Ecotoxicol. Environ. Saf. 2010, 73:1574-1579.
14. Faria M.S., Nogueira A.J.A., Soares A.M.V.M. The use of Chironomus riparius larvae to assess effects of pesticides from rice fields in adjacent freshwater ecosystems. Ecotoxicol. Environ. Saf. 2007, 67:218-226.
15. Thompson S., Budzinski H. Determination of polychlorinated biphenyls and chlorinated pesticides in environmental biological samples using focused microwave-assisted extraction. Int. J. Environ. Anal. Chem. 2000, 76:49-60.
16. Schmitz-Afonso I., Loyo-Rosales J.E., de la Paz Avilés M., Rattner B.A., Rice C.P. Determination of alkylphenol and alkylphenolethoxylates in biota by liquid chromatography with detection by tandem mass spectrometry and fluorescence spectroscopy. J. Chromatogr. A 2003, 1010:25-35.
17. Fernandez-Torres R., Bello Lopez M.A., Olias Consentino M., Callejon Mochon M., Ramos Payan M. Enzymatic-microwave assisted extraction and high-performance liquid chromatography-mass spectrometry for the determination of selected veterinary antibiotics in fish and mussel samples. J. Pharm. Biomed. Anal. 2011, 54:1146-1156.
18. Martínez Bueno M.J., Boillot C., Fenet H., Chiron S., Casellas C., Gómez E. Fast and easy extraction combined with high resolution-mass spectrometry for residue analysis of two anticonvulsants and their transformation products in marine mussels. J. Chromatogr. A 2013, 1305:27-34.
19. Sánchez-Avila J., Fernandez-Sanjuan M., Vicente J., Lacorte S. Development of a multi-residue method for the determination of organic micropollutants in water, sediment and mussels using gas chromatography-tandem mass spectrometry. J. Chromatogr. A 2011, 1218:6799-6811.
20. Lacroix C., Le Cuff N., Receveur J., Moraga D., Auffret M., Guyomarch J. Development of an innovative and "green" stir bar sorptive extraction-thermal desorption-gas chromatography-tandem mass spectrometry method for quantification of polycyclic aromatic hydrocarbons in marine biota. J. Chromatogr. A 2014, 1349:1-10.
21. Fernández-González V., Muniategui-Lorenzo S., López-Mahía P., Prada-Rodríguez D. Development of a programmed temperature vaporization-gas chromatography-tandem mass spectrometry method for polycyclic aromatic hydrocarbons analysis in biota samples at ultratrace levels. J. Chromatogr. A 2008, 1207:136-145.
22. Ali I., Gupta V.K., Aboul-Enein H.Y., Hussain A. Hyphenation in sample preparation: advancement from the micro to the nano world. J. Sep. Sci. 2008, 31:2040-2053.
23. Gómez-Ariza J.L., Bujalance M., Giráldez I., Velasco A., Morales E. Determination of polychlorinated biphenyls in biota samples using simultaneous pressurized liquid extraction and purification. J. Chromatogr. A 2002, 946:209-2019.
24. Bertin D., Ferrari B.J.D., Labadie P., Sapin A., Garric J., Budzinski H., Houde M., Babut M. Bioaccumulation of perfluoroalkyl compounds in midge (Chironomus riparius) larvae exposed to sediment. Environ. Pollut. 2014, 189:27-34.
25. Dévier M.-H., Labadie P., Togola A., Budzinski H. Simple methodology coupling microwave-assisted extraction to SPE/GC/MS for the analysis of natural steroids in biological tissues: application to the monitoring of endogenous steroids in marine mussels Mytilus sp. Anal. Chim. Acta 2010, 657:28-35.
26. Cueva-Mestanza R., Torres-Padron M.E., Sosa-Ferrera Z., Santana-Rodriguez J.J. Microwave-assisted micellar extraction coupled with solid-phase extraction for preconcentration of pharmaceuticals in molluscs prior to determination by HPLC. Biomed. Chromatogr. 2008, 22:1115-1122.
27. Saravanabhavan G., Helleur R., Hellou J. GC-MS/MS measurement of natural and synthetic estrogens in receiving waters and mussels close to a raw sewage ocean outfall. Chemosphere 2009, 76:1156-1162.
28. Anastassiades M., Lehotay S.J., Štajnbaher D., Schenck F.J. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. J. AOAC Int. 2003, 86:412-431.
29. Stubbings G., Bigwood T. The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC-MS/MS) procedure for the determination of veterinary drug residues in animal tissue using a QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach. Anal. Chim. Acta 2009, 637:68-78.
30. Abdallah H., Arnaudguilhem C., Jaber F., Lobinski R. Multiresidue analysis of 22 sulfonamides and their metabolites in animal tissues using quick, easy, cheap, effective, rugged, and safe extraction and high resolution mass spectrometry (hybrid linear ion trap-Orbitrap). J. Chromatogr. A 2014, 1355:61-72.
31. Wiest L., Bulete A., Giroud B., Fratta C., Amic S., Lambert O., Pouliquen H., Arnaudguilhem C. Multi-residue analysis of 80 environmental contaminants in honeys, honeybees and pollens by one extraction procedure followed by liquid and gas chromatography coupled with mass spectrometric detection. J. Chromatogr. A 2011, 1218:5743-5756.
32. Norli H.R., Christiansen A., Deribe E. Application of QuEChERS method for extraction of selected persistent organic pollutants in fish tissue and analysis by gas chromatography mass spectrometry. J. Chromatogr. A 2011, 1218:7234-7241.
33. Orlando E.A., Simionato A.V.C. Extraction of tetracyclinic antibiotic residues from fish filet: comparison and optimization of different procedures using liquid chromatography with fluorescence detection. J. Chromatogr. A 2013, 1307:111-118.
34. Rios A., Escarpa A., Simonet B. Analytical performance of miniaturized analytical systems. Miniaturization Anal. Syst. 2009, 357-363. John Wiley & Sons, Ltd.
35. Al-Othman Z.A., Ali I. Nano capillary electrophoresis in microchips: a need of the present century. J. Liq. Chromatogr. Relat. Technol. 2011, 34:1295-1325.
36. Ali I., Aboul-Enein H.Y., Gupta V.K. Nanochromatography and Nanocapillary Electrophoresis: Pharmaceutical and Environmental Analyses 2009, John Wiley & Sons, Hoboken.
37. Ali I., Al-Othman Z.A., Al-Warthan A., Aboul-Enein H.Y. Recent trends in chiral separations by nano liquid chromatography and nano capillary electrophoresis. Curr. Chromatogr. 2014, 1:81-89.
38. Ali I., Aboul-Enein H. Role of nano liquid chromatography in pharmaceutical analyses. Curr. Pharm. Anal. 2009, 5:367-380.
39. Karlsson K., Novotny M. Separation efficiency of slurry-packed liquid-chromatography microcolumns with very small inner diameters. Anal. Chem. 1988, 60:1662-1665.
40. Chervet J.P., Ursem M., Salzmann J.B. Instrumental requirements for nanoscale liquid chromatography. Anal. Chem. 1996, 68:1507-1512.
41. Vissers J.P.C. Recent developments in microcolumn liquid chromatography. J. Chromatogr. A 1999, 856:117-143.
42. Hsieh S.C., Jorgenson J.W. Preparation and evaluation of slurry-packed liquid chromatography microcolumns with inner diameters from 12 to 33mum. Anal. Chem. 1996, 68:1212-1217.
43. Schmidt A., Karas M., Dülcks T. Effect of different solution flow rates on analyte ion signals in nano-ESI MS, or: when does ESI turn into nano-ESI?. J. Am. Soc. Mass Spectrom. 2003, 14:492-500.
44. El-Faramawy A., Siu K.W.M., Thomson B.A. Efficiency of nano-electrospray ionization. J. Am. Soc. Mass Spectrom. 2005, 16:1702-1707.
45. Juraschek R., Dülcks T., Karas M. Nanoelectrospray - more than just a minimized-flow electrospray ionization source. J. Am. Soc. Mass Spectrom. 1999, 10:300-308.
46. Li R., Yu H., Xing R., Liu S., Qing Y., Li K., Li B., Meng X., Cui J., Li P. Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris. J. Chromatogr. B 2012, 899:86-95.
47. Clement C.C., Aphkhazava D., Nieves E., Callaway M., Olszewski W., Rotzschke O., Santambrogio L. Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics. J. Proteomics 2013, 78:172-187.
48. Jou Y.-J., Hua C.-H., Lin C.-D., Lai C.-H., Huang S.-H., Tsai M.-H., Kao J.-Y., Lin C.-W. S100A8 as potential salivary biomarker of oral squamous cell carcinoma using nanoLC-MS/MS. Clin. Chim. Acta 2014, 436:121-129.
49. Hu L., Boos K.-S., Ye M., Wu R., Zou H. Selective on-line serum peptide extraction and multidimensional separation by coupling a restricted-access material-based capillary trap column with nanoliquid chromatography-tandem mass spectrometry. J. Chromatogr. A 2009, 1216:5377-5384.
50. Kalay H., Ambrosini M., van Berkel P.H.C., Parren P.W.H.I., van Kooyk Y., Vallejo J.J.G. Online nanoliquid chromatography-mass spectrometry and nanofluorescence detection for high-resolution quantitative N-glycan analysis. Anal. Biochem. 2012, 423:153-162.
51. Gil G.-C., Brennan J., Throckmorton D.J., Branda S.S., Chirica G.S. Automated analysis of mouse serum peptidome using restricted access media and nanoliquid chromatography-tandem mass spectrometry. J. Chromatogr. B - Anal. Technol. Biomed. Life Sci. 2011, 879:1112-1120.
52. Besse J.-P., Garric J. Human pharmaceuticals in surface waters: implementation of a prioritization methodology and application to the French situation. Toxicol. Lett. 2008, 176:104-123.
53. Jean J., Perrodin Y., Pivot C., Trepo D., Perraud M., Droguet J., Tissot-Guerraz F., Locher F. Identification and prioritization of bioaccumulable pharmaceutical substances discharged in hospital effluents. J. Environ. Manage. 2012, 103:113-121.
54. Milic N., Milanovic M., Letic N.G., Sekulic M.T., Radonic J., Mihajlovic I., Miloradov M.V. Occurrence of antibiotics as emerging contaminant substances in aquatic environment. Int. J. Environ. Health Res. 2013, 23:296-310.
55. Li W.C. Occurrence, sources, and fate of pharmaceuticals in aquatic environment and soil. Environ. Pollut. 2014, 187:193-201.
56. Scott A.P. Do mollusks use vertebrate sex steroids as reproductive hormones? II. Critical review of the evidence that steroids have biological effects. Steroids 2013, 78:268-281.
57. Kroupova H.K., Trubiroha A., Lorenz C., Contardo-Jara V., Lutz I., Grabic R., Kocour M., Kloas W. The progestin levonorgestrel disrupts gonadotropin expression and sex steroid levels in pubertal roach (Rutilus rutilus). Aquat. Toxicol. 2014, 154:154-162.
58. Aris A.Z., Shamsuddin A.S., Praveena S.M. Occurrence of 17α-ethynylestradiol (EE2) in the environment and effect on exposed biota: a review. Environ. Int. 2014, 69:104-119.
59. Luo Y., Guo W., Ngo H.H., Nghiem L.D., Hai F.I., Zhang J., Liang S., Wang X.C. A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment. Sci. Total Environ. 2014, 473-474:619-641.
60. Gust M., Buronfosse T., Giamberini L., Ramil M., Mons R., Garric J. Effects of fluoxetine on the reproduction of two prosobranch mollusks: Potamopyrgus antipodarum and Valvata piscinalis. Environ. Pollut. 2009, 157:423-429.
61. E47 Committee Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates 2005, ASTM International.
62. OECD Test No. 219: Sediment-Water Chironomid Toxicity Using Spiked Water 2004, Organisation for Economic Co-operation and Development, Paris.
63. Gust M., Buronfosse T., Geffard O., Mons R., Queau H., Mouthon J., Garric J. In situ biomonitoring of freshwater quality using the New Zealand mudsnail Potamopyrgus antipodarum (Gray) exposed to waste water treatment plant (WWTP) effluent discharges. Water Res. 2010, 44:4517-4528.
64. Coulaud R., Geffard O., Xuereb B., Lacaze E., Quéau H., Garric J., Charles S., Chaumot A. In situ feeding assay with Gammarus fossarum (Crustacea): modelling the influence of confounding factors to improve water quality biomonitoring. Water Res. 2011, 45:6417-6429.
65. International Conference on Harmonization, Q2 (R1): Validation of Analytical procedures: Text and Methodology, November 2005.
66. Rainville P.D., Smith N.W., Cowan D., Plumb R.S. Comprehensive investigation of the influence of acidic, basic, and organic mobile phase compositions on bioanalytical assay sensitivity in positive ESI mode LC/MS/MS. J. Pharm. Biomed. Anal. 2012, 59:138-150.
67. Al-Qaim F.F., Abdullah M.P., Othman M.R., Latip J., Zakaria Z. Multi-residue analytical methodology-based liquid chromatography-time-of-flight-mass spectrometry for the analysis of pharmaceutical residues in surface water and effluents from sewage treatment plants and hospitals. J. Chromatogr. A 2014, 1345:139-153.
68. Robles-Molina J., Lara-Ortega F.J., Gilbert-López B., García-Reyes J.F., Molina-Díaz A. Multi-residue method for the determination of over 400 priority and emerging pollutants in water and wastewater by solid-phase extraction and liquid chromatography-time-of-flight mass spectrometry. J. Chromatogr. A 2014, 1350:30-43.
69. Salgueiro-González N., Turnes-Carou I., Muniategui-Lorenzo S., López-Mahía P., Prada-Rodríguez D. Membrane assisted solvent extraction coupled with liquid chromatography tandem mass spectrometry applied to the analysis of alkylphenols in water samples. J. Chromatogr. A 2013, 1281:46-53.
70. Dévier M.-H., Le Menach K., Viglino L., Di Gioia L., Lachassagne P., Budzinski H. Ultra-trace analysis of hormones, pharmaceutical substances, alkylphenols and phthalates in two French natural mineral waters. Sci. Total Environ. 2013, 443:621-632.
71. D'Orazio G., Cifuentes A., Fanali S. Chiral nano-liquid chromatography-mass to amino acids analysis for orange spectrometry applied juice profiling. Food Chem. 2008, 108:1114-1121.
72. Asensio-Ramos M., D'Orazio G., Hernandez-Borges J., Rocco A., Fanali S. Multi-walled carbon nanotubes-dispersive solid-phase extraction combined with nano-liquid chromatography for the analysis of pesticides in water samples. Anal. Bioanal. Chem. 2011, 400:1113-1123.
73. AOAC Official Method 2007.01, "Pesticides residues in food by acetonitrile extraction and partitionning with magnesium sulphate".
74. Norme NF EN 15662 Aliments D'origine Végétale, Méthode Polyvalente de Détermination Des Résidus Des Pesticides Par CG-SM et SL/SM/SM Avec Extraction/partition Avec de L'acétonitrile et Nettoyage Par SPE Dispersés - AFNOR, 2009.
75. Giroud B., Vauchez A., Vulliet E., Wiest L., Buleté A. Trace level determination of pyrethroid and neonicotinoid insecticides in beebread using acetonitrile-based extraction followed by analysis with ultra-high-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 2013, 1316:53-61.
76. Jia F., Wang W., Wang J., Yin J., Liu Y., Liu Z. New strategy to enhance the extraction efficiency of pyrethroid pesticides in fish samples using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. Anal. Methods 2012, 4:449-453.
77. Przybylski C., Segard C. Method for routine screening of pesticides and metabolites in meat based baby-food using extraction and gas chromatography-mass spectrometry. J. Sep. Sci. 2009, 32:1858-1867.
78. Salvia M.-V., Vulliet E., Wiest L., Baudot R., Cren-Olivé C. Development of a multi-residue method using acetonitrile-based extraction followed by liquid chromatography-tandem mass spectrometry for the analysis of steroids and veterinary and human drugs at trace levels in soil. J. Chromatogr. A 2012, 1245:122-133.
79. Dickson L.C. Performance characterization of a quantitative liquid chromatography-tandem mass spectrometric method for 12 macrolide and lincosamide antibiotics in salmon, shrimp and tilapia. J. Chromatogr. B 2014, 967:203-210.
80. Gago-Ferrero P., Díaz-Cruz M.S., Barceló D. Multi-residue method for trace level determination of UV filters in fish based on pressurized liquid extraction and liquid chromatography-quadrupole-linear ion trap-mass spectrometry. J. Chromatogr. A 2013, 1286:93-101.
81. Bonfiglio R., King R.C., Olah T.V., Merkle K. The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compounds. Rapid Commun. Mass Spectrom. 1999, 13:1175-1185.
82. Matuszewski B.K. Standard line slopes as a measure of a relative matrix effect in quantitative HPLC-MS bioanalysis. J. Chromatogr. B 2006, 830:293-300.
83. Salgueiro-González N., Turnes-Carou I., Muniategui-Lorenzo S., López-Mahía P., Prada-Rodríguez D. Fast and selective pressurized liquid extraction with simultaneous in cell clean up for the analysis of alkylphenols and bisphenol A in bivalve molluscs. J. Chromatogr. A 2012, 1270:80-87.
84. Salgueiro-González N., Concha-Graña E., Turnes-Carou I., Muniategui-Lorenzo S., López-Mahía P., Prada-Rodríguez D. Blank and sample handling troubleshooting in ultratrace analysis of alkylphenols and bisphenol A by liquid chromatography tandem mass spectrometry. Talanta 2012, 101:413-419.
85. Gauthier-Clerc S., Pellerin J., Amiard J.C. Estradiol-17β and testosterone concentrations in male and female Mya arenaria (Mollusca bivalvia) during the reproductive cycle. Gen. Comp. Endocrinol. 2006, 145:133-139.
86. Siah A., Pellerin J., Amiard J.-C., Pelletier E., Viglino L. Delayed gametogenesis and progesterone levels in soft-shell clams (Mya arenaria) in relation to in situ contamination to organotins and heavy metals in the St. Lawrence River (Canada). Comp. Biochem. Physiol. Part C Toxicol. Pharmacol. 2003, 135:145-156.
87. Morcillo Y., Porte C. Evidence of endocrine disruption in clams - Ruditapes decussata - transplanted to a tributyltin-polluted environment. Environ. Pollut. 2000, 107:47-52.
88. Warrier S.R., Tirumalai R., Subramoniam T. Occurrence of vertebrate steroids, estradiol 17β and progesterone in the reproducing females of the mud crab Scylla serrata. Comp. Biochem. Physiol. A. Mol. Integr. Physiol. 2001, 130:283-294.
89. Osada M., Tawarayama H., Mori K. Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis: gonadal profile and immunolocalization of P450 aromatase and estrogen. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2004, 139:123-128.
90. Pojana G., Gomiero A., Jonkers N., Marcomini A. Natural and synthetic endocrine disrupting compounds (EDCs) in water, sediment and biota of a coastal lagoon. Environ. Int. 2007, 33:929-936.
91. Peck M.R., Labadie P., Minier C., Hill E.M. Profiles of environmental and endogenous estrogens in the zebra mussel Dreissena polymorpha. Chemosphere 2007, 69:1-8.
92. Araujo P. Key aspects of analytical method validation and linearity evaluation. J. Chromatogr. B 2009, 877:2224-2234.