Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes

PLoS ONE

Volumn 9, Issue 10, 2014, Pages

  De Hoog, Hans Peter M ^a   JieRong, Esther M Lin ^a   Banerjee, Sourabh ^a   Décaillot, Fabien M ^b   Nallani, Madhavan ^a,c  

^a Research Biolabs Pte Ltd   (Singapore)

^b AGENCY FOR SCIENCE   (Singapore)

^c NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ARTIFICIAL MEMBRANE; CHEMOKINE RECEPTOR CXCR4; CXCR4 PROTEIN, HUMAN;

ANIMAL; ARTIFICIAL MEMBRANE; CELL FREE SYSTEM; CHEMISTRY; GENETIC PROCEDURES; HUMAN; MOUSE; PROTEIN CONFORMATION; PROTEIN FOLDING;

ANIMALS; ANTIBODIES; BIOSENSING TECHNIQUES; CELL-FREE SYSTEM; HUMANS; MEMBRANES, ARTIFICIAL; MICE; PROTEIN CONFORMATION; PROTEIN FOLDING; RECEPTORS, CXCR4;

EID: 84908200314     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0110847     Document Type: Article

References (25)

1. Robertson N, Jazayeri A, Errey J, Baig A, Hurrell E, et al. (2010) The properties of thermostabilised G protein-coupled receptors (StaRs) and their use in drug discovery. Neuropharmacology 60: 36-44.
2. Wu B, Chien EYT, Mol CD, Fenalti G, Liu W, Katritch V, et al. (2010) Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science 330: 1066-1071.
3. Discher DE, Eisenberg A (2002) Polymer vesicles. Science 297: 967-973.
4. Discher BM, Won YY, Ege DS, Lee JCM, Bates FS, et al. (1999) Polymersomes: Tough vesicles made from diblock copolymers. Science 284: 1143-1146.
5. Oates J, Watts A (2011) Uncovering the intimate relationship between lipids, cholesterol and GPCR activation. Curr Opin Struct Biol 21: 802-807.
6. Phillips R, Ursell T, Wiggins P, Sens P (2009) Emerging roles for lipids in shaping membrane-protein function. Nature 459: 379-385.
7. Kumar M, Grzelakowski M, Zilles J, Clark M, Meier W (2007) Highly permeable polymeric membranes based on the incorporation of the functional water channel protein Aquaporin Z. PNAS 104: 20719-20724.
8. Nallani M, Benito S, Onaca O, Graff A, Lindemann M, et al (2006) A nanocompartment system (Synthosome) designed for biotechnological applications. J Biotechnol 123: 50-59.
9. Choi HJ, Montemagno CD (2005) Artificial organelle: ATP synthesis from cellular mimetic polymersomes. Nano Lett 5: 2538-2542.
10. Kita-Tokarczyk K, Grumelard J, Haefele T, Meier W (2005) Block copolymer vesicles - Using concepts from polymer chemistry to mimic biomembranes. Polymer 46: 3540-3563.
11. Nallani M, Andreasson-Ochsner M, Tan CWD, Sinner EK, Wisantoso Y, et al. (2011) Proteopolymersomes: In vitro production of a membrane protein in polymersome membranes. Biointerphases 6: 153-157.
12. May S, Andreasson-Ochsner M, Fu Z, Low YX, Tan D, et al. (2013) In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies. Angew. Chem. - Int. Ed. 52: 749-753.
13. Allen SJ, Crown SE, Handel TM (2007) Chemokine: Receptor structure, interactions, and ntagonism Annu. Rev. Immunol. 25: 787-820.
14. Karlsson OP, Löfås S (2002) Flow-mediated on-surface reconstitution of Gprotein coupled receptors for applications in surface plasmon resonance biosensors. Anal. Biochem. 300: 132-138.
15. Stenlund P, Babcock GJ, Sodroski J, Myszka DG (2003) Capture and reconstitution of G protein-coupled receptors on a biosensor surface. Anal. Biochem. 316: 243-250.
16. Navratilova I, Sodroski J, Myszka DG (2005) Solubilization, stabilization, and purification of chemokine receptors using biosensor technology. Anal. Biochem. 339: 271-281.
17. Knoll W (1998) Interfaces and thin films as seen by bound electromagnetic waves. Ann. Rev. Phys. Chem. 49: 569-638.
18. Strizki JM, Turner JD, Collman RG, Hoxie J, González-Scarano F (1987) A monoclonal antibody (12G5) directed against CXCR-4 inhibits infection with the dual-tropic human immunodeficiency virus type 1 isolate HIV-1(89.6) but not the T-tropic isolate HIV-1(HxB). J. Virol. 71: 5678-5683.
19. Carnec X, Quan L, Olson WC, Hazan U, Dragic T (2005) Anti-CXCR4 monoclonal antibodies recognizing overlapping epitopes differ significantly in their ability to inhibit entry of human immunodeficiency virus type 1. J. Virol. 79: 1930-1933.
20. Willis S, Davidoff C, Schilling J, Wanless A, Doranz BJ, et al (2008) Virus-like particles as quantitative probes of membrane protein interactions. Biochemistry 47: 6988-6990.
21. Hoffman TL, Canziani G, Jia L, Rucker J, Doms RW (2000) A biosensor assay for studying ligand-membrane receptor interactions: Binding of antibodies and HIV-1 Env chemokine receptors. PNAS 97: 11215-11220.
22. Whorton MR, Bokoch MP, Rasmussen SGF, Huang B, Zare RN, et al. (2007) A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. PNAS 104: 7682-7687.
23. Baribaud F, Edwards TG, Sharron M, Brelot A, Heveker N, et al. (2007) Antigenically distinct conformations of CXCR4. J. Virol. 75: 8957-8967.
24. Yaşayan G, Redhead M, Magnusson JP, Spain SG, Allen S, et al. (2012) Welldefined polymeric vesicles with high stability and modulation of cell uptake by a simple coating protocol. Polym. Chem. 9: 2596-2604.
25. Hanson MA, Cherezov V, Griffith MT, Roth CB, Jaakola VP, et al. (2008) A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor. Structure 16: 897-905.