Comparison of bacteriological culture and PCR for detection of bacteria in ovine milk-Sheep are not small cows

Journal of Dairy Science

Volumn 97, Issue 10, 2014, Pages 6326-6333

  Zadoks, Ruth N ^a,b   Tassi, Riccardo ^a   Martin, Elena ^a   Holopainen, Jani ^c   McCallum, Sarah ^d   Gibbons, James ^e   Ballingall, Keith T ^a  

^a MOREDUN RESEARCH INSTITUTE   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c Thermo Fisher Scientific Oy   (Finland)

^d BIOBEST LABORATORIES LTD   (United Kingdom)

^e UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Culture; Mastitis; PCR; Sheep

Indexed keywords

OVIS; OVIS ARIES;

ANIMAL; BACTERIUM; CLASSIFICATION; COMPARATIVE STUDY; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MASTITIS; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MILK; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASE; SPECIES DIFFERENCE; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; UDDER; VETERINARY;

ANIMALS; BACTERIA; BACTERIOLOGICAL TECHNIQUES; FEMALE; MAMMARY GLANDS, ANIMAL; MASTITIS; MILK; REAL-TIME POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES; SPECIES SPECIFICITY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS;

EID: 84908054788     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2014-8351     Document Type: Article

References (32)

1. Aarestrup F.M., Wegener H.C., Collignon P. Resistance in bacteria of the food chain: Epidemiology and control strategies. Expert Rev. Anti. Infect. Ther. 2008, 6:733-750.
2. Arsenault J., Dubreuil P., Higgins R., Bélanger D. Risk factors and impacts of clinical and subclinical mastitis in commercial meat-producing sheep flocks in Quebec, Canada. Prev. Vet. Med. 2008, 87:373-393.
3. Cowan and Steel's Manual for the Identification of Medical Bacteria 2004, Cambridge University Press, Cambridge, UK. 3rd. G.I. Barrow, R.K.A. Feltham (Eds.).
4. Bergonier D., de Crémoux R., Rupp R., Lagriffoul G., Berthelot X. Mastitis of dairy small ruminants. Vet. Res. 2003, 34:689-716.
5. Brakstad O.G., Aasbakk K., Maeland J.A. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol. 1992, 30:1654-1660.
6. Callon C., Duthoit F., Delbès C., Ferrand M., Le Frileux Y., De Crémoux R., Montel M.-C. Stability of microbial communities in goat milk during a lactation year: Molecular approaches. Syst. Appl. Microbiol. 2007, 30:547-560.
7. Cederlöf S.E., Toft N., Aalbaek B., Klaas I.C. Latent class analysis of the diagnostic characteristics of PCR and conventional bacteriological culture in diagnosing intramammary infections caused by Staphylococcus aureus in dairy cows at dry off. Acta Vet. Scand. 2012, 54:65-71.
8. Conington J., Cao G., Stott A., Bünger L. Breeding for resistance to mastitis in United Kingdom sheep, a review and economic appraisal. Vet. Rec. 2008, 162:369-376.
9. Cuccuru C., Meloni M., Sala E., Scaccabarozzi L., Locatelli C., Moroni P., Bronzo V. Effects of intramammary infections on somatic cell score and milk yield in Sarda sheep. N. Z. Vet. J. 2011, 59:128-131.
10. Drancourt M., Raoult D. rpoB gene sequence-based identification of Staphylococcus species. J. Clin. Microbiol. 2002, 40:1333-1338.
11. Fotou K., Tzora A., Voidarou Ch., Alexopoulos A., Plessas S., Avgeris I., Bezirtzoglou E., Akrida-Demertzi K., Demertzis P.G. Isolation of microbial pathogens of subclinical mastitis from raw sheep's milk of Epirus (Greece) and their role in its hygiene. Anaerobe 2011, 17:315-319.
12. Gilchrist T.L., Smith D.G., Fitzpatrick J.L., Zadoks R.N., Fontaine M.C. Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates. J. Dairy Sci. 2013, 96:962-970.
13. Gillespie B.E., Oliver S.P. Simultaneous detection of mastitis pathogens, Staphylococcus aureusStreptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. J. Dairy Sci. 2005, 88:3510-3518.
14. Heikens E., Fleer A., Paauw A., Florijn A., Fluit A.C. Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci. J. Clin. Microbiol. 2005, 43:2286-2290.
15. Jenness R. Biochemical and nutritional aspects of milk and colostrum. Lactation 1985, 164-197. Iowa State University Press, Ames. B.L. Larson (Ed.).
16. Keane O.M., Budd K.E., Flynn J., McCoy F. Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture. Vet. Rec. 2013, 173:268-274.
17. Koskinen M.T., Holopainen J., Pyörälä S., Bredbacka P., Pitkälä A., Barkema H.W., Bexiga R., Roberson J., Sølverød L., Piccinini R., Kelton D., Lehmusto H., Niskala S., Salmikivi L. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci. 2009, 92:952-959.
18. Koskinen M.T., Wellenberg G.J., Sampimon O.C., Holopainen J., Rothkamp A., Salmikivi L., van Haeringen W.A., Lam T.J., Pyörälä S. Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. J. Dairy Sci. 2010, 93:5707-5715.
19. Mellmann A., Becker K., von Eiff C., Keckevoet U., Schumann P., Harmsen D. Sequencing and staphylococci identification. Emerg. Infect. Dis. 2006, 12:333-336.
20. Mørk T., Waage S., Tollersrud T., Kvitle B., Sviland S. Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Vet. Scand. 2007, 49:23.
21. Moroni P., Pisoni G., Varisco G., Boettcher P. Effect of intramammary infection in Bergamasca meat sheep on milk parameters and lamb growth. J. Dairy Res. 2007, 74:340-344.
22. Omaleki L., Browning G.F., Allen J.L., Barber S.R. The role of Mannheimia species in ovine mastitis. Vet. Microbiol. 2011, 153:67-72.
23. Pérez M.M., Prenafeta A., Valle J., Penadés J., Rota C., Solano C., Marco J., Grilló M.J., Lasa I., Irache J.M., Maira-Litran T., Jiménez-Barbero J., Costa L., Pier G.B., de Andrés D., Amorena B. Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl β-1,6 glucosamine specific antibody production using biofilm-embedded bacteria. Vaccine 2009, 27:2379-2386.
24. Phuektes P., Mansell P.D., Browning G.F. Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. J. Dairy Sci. 2001, 84:1140-1148.
25. Quigley L., O'Sullivan O., Stanton C., Beresford T.P., Ross R.P., Fitzgerald G.F., Cotter P.D. The complex microbiota of raw milk. FEMS Microbiol. Rev. 2013, 37:664-698.
26. Smyth D.S., Feil E.J., Meaney W.J., Hartigan P.J., Tollersrud T., Fitzgerald J.R., Enright M.C., Smyth C.J. Molecular genetic typing reveals further insights into the diversity of animal-associated Staphylococcus aureus. J. Med. Microbiol. 2009, 58:1343-1353.
27. Spittel S., Hoedemaker M. Mastitis diagnosis in dairy cows using PathoProof real-time polymerase chain reaction assay in comparison with conventional bacterial culture in a Northern German field study. Berl. Munch. Tierärztl. Wochenschr. 2012, 125:494-502.
28. Supré K., De Vliegher S., Cleenwerck I., Engelbeen K., Van Trappen S., Piepers S., Sampimon O.C., Zadoks R.N., De Vos P., Haesebrouck F. Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows. Int. J. Syst. Evol. Microbiol. 2010, 60:2739-2744.
29. Taponen S., Salmikivi L., Simojoki H., Koskinen M.T., Pyörälä S. Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J. Dairy Sci. 2009, 92:2610-2617.
30. Tornadijo E., Fresno J.M., Carballo J., Martín-Sarmiento R. Study of Enterobacteriaceae throughout the manufacturing and ripening of hard goats' cheese. J. Appl. Bacteriol. 1993, 75:240-246.
31. Wiedmann M., Weilmeier D., Dineen S.S., Ralyea R., Boor K.J. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk. Appl. Environ. Microbiol. 2000, 66:2085-2095.
32. Zadoks R.N., Watts J.L. Species identification of coagulase-negative staphylococci: Genotyping is superior to phenotyping. Vet. Microbiol. 2009, 134:20-28.