Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Brazilian Journal of Microbiology

Volumn 45, Issue 2, 2014, Pages 495-501

  Souto, Renata ^a   Silva Boghossian, Carina M ^a   Colombo, Ana Paula Vieira ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Acinetobacter spp; PCR; Periodontitis; Pseudomonas aeruginosa; Saliva; Subgingival biofilm

Indexed keywords

ACINETOBACTER; PSEUDOMONAS AERUGINOSA;

BACTERIAL DNA;

ACINETOBACTER; ADULT; BIOFILM; COMPARATIVE STUDY; FEMALE; GENETICS; GINGIVA; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MIDDLE AGED; NORMAL HUMAN; PERIODONTAL DISEASE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PREVALENCE; PSEUDOMONAS AERUGINOSA; SALIVA; YOUNG ADULT;

ACINETOBACTER; ADULT; BIOFILMS; DNA, BACTERIAL; FEMALE; GINGIVA; HEALTHY VOLUNTEERS; HUMANS; MALE; MIDDLE AGED; PERIODONTAL DISEASES; POLYMERASE CHAIN REACTION; PREVALENCE; PSEUDOMONAS AERUGINOSA; SALIVA; YOUNG ADULT;

EID: 84908043967     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822014000200017     Document Type: Article

References (43)

1. Abe S., Ishihara K, Okuda K (2001) Prevalence of potential respiratory pathogens in the mouths of elderly patients and effects of professional oral care. Arch Gerontol Geriatr 32: 45-55.
2. Agodi A., Barchitta M, Cipresso R, Giaquinta L, Romeo MA, Denaro C (2007) Pseudomonas aeruginosa carriage, colonization, and infection in ICU patients. Intensive Care Med 33:1155-1161.
3. Ali R.W.C., Bakken V, Nilsen R, Skaug, N (1994) Comparative detection frequency of 6 putative periodontal pathogens in Sudanese and Norwegian adult periodontitis patients. J Periodontol 65:1046-1052.
4. Ali R.W.C., Velcescu MC, Jivanescu B, Lophtus B, Skaug N (1996) Prevalence of 6 putative periodontal pathogens in subgingival plaque samples from Romanian adult periodontitis patients. J Clin Periodonto. 18:411-415.
5. Barbosa F.C., Mayer MP, Saba-Chujfi E, Cai S (2001) Subgingival occurrence and antimicrobial susceptibility of enteric rods and pseudomonads from Brazilian periodontitis patients. Oral Microbiol Immunol 16:306-310.
6. Bergogne-Berezin E., Towner KJ (1996) Acinetobacter spp. as nosocomial pathogens: Microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9:148-165.
7. Botero J.E., Contreras A, Lafaurie G, Jaramillo A, Betancourt M, Arce RM (2007) Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. J Periodontol 78:696-704.
8. Broderick N.A., Raffa, KF, Goodman RM, Handelsman J (2004) Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods. Appl Environ Microbiol 70:293-300.
9. Chastre J., Fagon J (2002) Ventilator-associated pneumonia. Am J Respir Crit Care Med 165:867-903.
10. Colombo A.P., Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD et al. (2009) Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 80:1421-1432.
11. Colombo A.P., Haffajee AD, Dewhirst FE, Paster BJ, Smith CM, Cugini M A et al. (1998) Clinical and microbiological features of refractory periodontitis subjects. J Clin Periodontol 25:169-180.
12. Colombo A.P., Teles RP, Torres MC, Souto R, Rosalem WJ, Mendes MC et al. (2002) Subgingival microbiota of Brazilian subjects with untreated chronic periodontitis. J Periodontol 73:360-369.
13. Da Silva-Boghossian CM, Souto R, Luiz RR, Colombo APV (2011) Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal diaseases. Arch Oral Biol 56:899-906.
14. De Vos D, Lim JrA, Pirnay JP, Struelens M, Vandenvelde C, Duinslaeger L et al. (1997) Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J Clin Microbiol 35:1295-1299.
15. Fourrier F., Duvivier B, Boutigny H, Roussel-Delvallez M, Chopin C (1998) Colonization of dental plaque: A source of nosocomial infections in intensive care unit patients. Crit Care Me. 26:301-308.
16. Fournier P.E., Vallenet D, Barbe V, Audic S, Ogata H, Poirel L et al. (2006) Comparative genomics of multidrug resistance in Acinetobacter baumannii. PLoS Genet. 10:2-7.
17. Fritschi B.Z., Albert-Kiszely A, Persson GR (2008) Staphylococcus aureus and other bacteria in untreated periodontitis. J Dent Re. 87:589-593.
18. Hansen S.K., Haagensen JA, Gjermansen M, Jorgensen T. M, Tolker-Nielsen T., Molin S (2007) Characterization of a Pseudomonas putida rough variant evolved in a mixedspecies biofilm with Acinetobacter sp. strain C6. J Bacteriol 189:4932-4493.
19. Goncalves LdeS, Souto R, Colombo AP (2009) Detection of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival biofilm of HIV-infected subjects undergoing HAART with chronic periodontitis. Eur J Clin Microbiol Infect Dis 28:1335-1342.
20. Karlowsky J.A., Draghi DC, Jones ME, Thornsberry C, Friedland IR, Sahm D. F (2003) Surveillance for antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from hospitalized patients in the United States, 1998 to 2001. Antimicrob Agents Chemother 47:1681-1688.
21. Laine M.J., Farre MA, Crusius BA, Pena S (2000) The mouthwash: A non- invasive sampling method to study cytokine gene polymorphisms. J. Periodontol 71:1315-1318.
22. Luna C.M., Aruj PK (2007) Nosocomial Acinetobacter pneumonia. Respirology 12:787-791.
23. Obana Y (1986) Pathogenic significance of Acinetobacter calcoaceticus: Analysis of experimental infection in mice. Microbiol Immunol 30:645-657.
24. Page R.C., Kornman, KS (1997) The pathogenesis of human periodontitis: An introduction. Periodontol 2000 14:9-11.
25. Paju P. Scannapieco FA (2007) Oral biofilms, periodontitis, and pulmonary infections. Oral Diseases 13:508-512.
26. Paster B.J., Olsen I, Aas JA, Dewhirst FE (2006) The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000 42:80-87.
27. Persson G.R., Hitti J, Paul K, Hirschi R, Weibel M, RothenMet al. (2008) Tannerella forsythia and Pseudomonas aeruginosa in subgingival bacterial samples from parous women. J Periodontol 79:508-516.
28. Pihl M.L.E.C., Schimidtchen A, Svensater G, Davies JR (2010) Effects of clinical isolates of Pseudomonas aeruginosa on Staphylococcus epidermidis biofilm formation. FEMS Immunol Med Microbiol 59:504-512.
29. Scannapieco FA (1998) Position paper of The American Academy of Periodontology: Periodontal disease as a potential risk factor for systemic diseases. J Periodontol 69:841-850.
30. Scannapieco F.A., Papandonatos GD, Dunford RG (1998) Associations between oral conditions and respiratory disease in a national sample survey population. Ann. Periodontol 3:251-256.
31. Slots J., Feik D, Rams TE (1990) Prevalence and antimicrobial susceptibility of Enterobacteriacea, Psedomonadaceaea and Acinetobacter in human periodontitis. Oral Microbiol Immunol 5:149-154.
32. Slots J., Rams TE, Litsgarten MA (1988) Yeasts, enteric rods and pseudomonads in the subgingival flora of severe adult periodontitis. Oral Microb Immunol 3:47-52.
33. Slots J., Rams TE, Feik D, Taveras HD, Gillespie GM (1991) Subgingival microflora of advanced periodontitis in the Dominican Republic. Oral Microbiol Immunol 62:543-547.
34. Smith R.S., Iglewski BH (2003) P. aeruginosa quorum-sensing systems and virulence. Curr Opin Microbiol 6:56-60.
35. Socransky S.S., Haffajee AD, Cugini MA, Smith C, Kent JrRL (1998) Microbial complexes in subgingival plaque. J Clin Periodontol 25:134-144.
36. Souto R., Colombo AP (2008) Detection of Helicobacter pylori by polymerase chain reaction in the subgingival biofilm and saliva of non-dyspeptic periodontal patients. J Periodontol 79:97-103.
37. Souto R., de Andrade AFB, Uzeda M, Colombo APV (2006) Prevalence of "non-oral" pathogenic bacteria in subgingival biofilm of subjects with chronic periodontitis. Braz J Microbiol 37:208-215.
38. Towner KJ (1997) Clinical importance and antibiotic resistance of Acinetobacter spp. J Med Microbiol 46:721-746.
39. Vanbroekhoven K., Ryngaert AK, Wattiau P, Mot R, Springael D (2004) Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting. FEMS Microbiol Ecol 50:37-50.
40. Wagner V.E., Iglewski BH (2008) P. aeruginosa biofilms in CF infection. Clin. Rev Allerg Immul 35:124-134.
41. Willis S.G., Smith KS, Dunn VL, Gapter LA, Riviere KH, Riviere GR (1999) Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR. J Clin Microbiol 37:867-869.
42. Woods D.E., Cryz SJ, Friedman RL, Iglewski BH (1982) Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats. Infect Immun 36:1223-1228.
43. Zuanazzi D., Souto R, Mattos MB, Zuanazzi MR, Tura BR, Sansone C, Colombo AP (2010) Prevalence of potential bacterial respiratory pathogens in the oral cavity of hospitalised individuals. Arch Oral Biol 55:21-28.