A new target for the old regulator: H-NS suppress T6SS secretory protein EvpP, the major virulence factor in the fish pathogen Edwardsiella tarda

Letters in Applied Microbiology

Volumn 59, Issue 5, 2014, Pages 557-564

  Zhang, J ^a   Xiao, J ^a   Zhang, Y ^a   Cui, S ^a   Liu, Q ^a   Wang, Q ^a   Wu, H ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Edwardsiella tarda; EvpP; H NS; Regulation; T6SS

Indexed keywords

FISH; LOSSES; PATHOGENS; PROTEINS;

EDWARDSIELLUM TARDA; EVPP; GLOBAL REGULATORS; H-NS; HOST-CELL INVASION; PROMOTER REGION; REGULATION; T6SS; TWO COMPONENT SYSTEMS; VIRULENCE FACTORS;

TRANSCRIPTION;

EVPP PROTEIN; HISTONE LIKE NUCLEOID STRUCTURING PROTEIN; SECRETORY PROTEIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR; BACTERIAL PROTEIN; DNA BINDING PROTEIN; H-NS PROTEIN, BACTERIA;

ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; DNA FOOTPRINTING; DOWN REGULATION; EDWARDSIELLA TARDA; FISH; GENE EXPRESSION REGULATION; GENE INTERACTION; GENE MAPPING; GENE REPRESSION; IN VITRO STUDY; NONHUMAN; PROMOTER REGION; TRANSCRIPTION REGULATION; TYPE IV SECRETION SYSTEM; CHEMISTRY; GENETICS; METABOLISM; PATHOGENICITY;

EDWARDSIELLA TARDA;

BACTERIAL PROTEINS; DNA-BINDING PROTEINS; EDWARDSIELLA TARDA; GENE EXPRESSION REGULATION, BACTERIAL; VIRULENCE FACTORS;

EID: 84907960895     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/lam.12316     Document Type: Article

References (26)

1. Bouffartigues, E., Buckle, M., Badaut, C., Travers, A. and Rimsky, S. (2007) H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing. Nat Struct Mol Biol 14, 441-448.
2. Cordeiro, T.N., Garci, A.J., Pons, J.I., Aznar, S., Juarez, A. and Pons, M. (2008) A single residue mutation in Hha preserving structure and binding to H-NS results in loss of H-NS mediated gene repression properties. FEBS Lett 582, 3139-3144.
3. Dennis, J.J. and Zylstra, G.J. (1998) Plasposons: modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl Environ Microbiol 64, 2710-2715.
4. Dorman, C.J. (2004) H-NS: a universal regulator for a dynamic genome. Nat Rev Microbiol 2, 391-400.
5. Dorman, C.J. (2007) H-NS, the genome sentinel. Nat Rev Microbiol 5, 157-161.
6. Fang, F.C. and Rimsky, S. (2008) New insights into transcriptional regulation by H-NS. Curr Opin Microbiol 11, 113-120.
7. Fass, E. and Groisman, E.A. (2009) Control of Salmonella pathogenicity island-2 gene expression. Curr Opin Microbiol 12, 199-204.
8. Gordon, B.R.G., Li, Y., Cote, A., Weirauch, M.T., Ding, P., Hughes, T.R., Navarre, W.W., Xia, B. et al. (2011) Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins. Proc Natl Acad Sci USA 108, 10690-10695.
9. Lang, B., Blot, N., Bouffartigues, E., Buckle, M., Geertz, M., Gualerzi, C.O., Mavathur, R., Muskhelishvili, G. et al. (2007) High affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes. Nucleic Acids Res 35, 6330-6337.
10. Liang, W.L., Wang, S.X., Yu, F.G., Zhang, L.J., Qi, G.M., Liu, Y.Q., Gao, S.Y. and Kan, B. (2003) Construction and evaluation of a safe, live, oral Vibrio cholerae vaccine candidate, IEM108. Infect Immun 71, 5498-5504.
11. Lim, C.J., Lee, S.Y., Kenney, L.J. and Yan, J. (2012) Nucleoprotein filament formation is the structural basis for bacterial protein H-NS gene silencing. Sci Rep 2, 509.
12. Liu, Y., Chen, H., Kenney, L.J. and Yan, J. (2010) A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes. Genes Dev 24, 339-344.
13. Lucchini, S., Rowley, G., Goldberg, M.D., Hurd, D., Harrison, M. and Hinton, J.C. (2006) H-NS mediates the silencing of laterally acquired genes in bacteria. PLoS Pathog 2, e81.
14. Luijsterburg, M.S., Noom, M.C., Wuite, G.J.L. and Dame, R.T. (2006) The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: a molecular perspective. J Struct Biol 156, 262-272.
15. Mohanty, B. and Sahoo, P. (2007) Edwardsiellosis in fish: a brief review. J Biosci 32, 1331-1344.
16. Navarre, W.W., McClell, M., Libby, S.J. and Fang, F.C. (2007) Silencing of xenogeneic DNA by H-NS-facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA. Genes Dev 21, 1456-1471.
17. Tan, Y.P., Zheng, J., Tung, S.L., Rosenshine, I. and Leung, K.Y. (2005) Role of type III secretion in Edwardsiella tarda virulence. Microbiology 151, 2301-2313.
18. Tendeng, C. and Bertin, P.N. (2003) H-NS in Gram-negative bacteria: a family of multifaceted proteins. Trends Microbiol 11, 511-518.
19. Wang, X., Wang, Q.Y., Xiao, J.F., Liu, Q., Wu, H.Z., Xu, L.L. and Zhang, Y.X. (2009a) Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish Shellfish Immunol 27, 469-477.
20. Wang, Q.Y., Yang, M.J., Xiao, J.F., Wu, H.Z., Wang, X., Lv, Y.Z., Xu, L.L., Zheng, H.J. et al. (2009b) Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One 4, e7646.
21. Wang, X., Wang, Q.Y., Xiao, J.F., Liu, Q., Wu, H.Z. and Zhang, Y.X. (2010) Hemolysin EthA in Edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system EsrA-EsrB and nucleoid protein HhaEt. Fish Shellfish Immunol 29, 1082-1091.
22. Wang, Y., Cen, X.F., Zhao, G.P. and Wang, J. (2012) Characterization of a new GlnR binding box in the promoter of amtB in Streptomyces coelicolor inferred a PhoP/GlnR competitive binding mechanism for transcriptional regulation of amtB. J Bacteriol 194, 5237.
23. Xiao, J.F., Wang, Q.Y., Liu, Q., Wang, X., Liu, H. and Zhang, Y.X. (2009) Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquac Res 40, 13-17.
24. Xiao, Y., Liu, Q., Chen, H. and Zhang, Y.X. (2011) A stable plasmid system for heterologous antigen expression in attenuated Vibrio anguillarum. Vaccine 29, 6986-6993.
25. Yang, M.J., Lv, Y.Z., Xiao, J.F., Wu, H.Z., Zheng, H.J., Liu, Q., Zhang, Y.X. and Wang, Q.Y. (2012) Edwardsiella comparative phylogenomics reveal the new intra/inter-Species taxonomic relationships, virulence evolution and niche adaptation mechanism. PLoS One 7, e36987.
26. Zheng, J. and Leung, K.Y. (2007) Dissection of a type VI secretion system in Edwardsiella tarda. Mol Microbiol 66, 1192-1206.