Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency

Microchimica Acta

Volumn 181, Issue 13-14, 2014, Pages 1689-1696

  Ruijter, Jan M ^a   Lorenz, Peter ^b   Tuomi, Jari M ^c   Hecker, Michael ^d   van den Hoff, Maurice J B ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF ROSTOCK   (Germany)

^c NORTHERN ONTARIO SCHOOL OF MEDICINE   (Canada)

^d Steinbeis Transfer Center for Proteome Analysis   (Germany)

Author keywords

DNA binding dyes; Hybridization probes; Hydrolysis probes; Monitoring chemistry; PCR efficiency; Quantitative PCR

Indexed keywords

EID: 84907860992     PISSN: 00263672     EISSN: 14365073     Source Type: Journal    
DOI: 10.1007/s00604-013-1155-8     Document Type: Article

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