MNAzyme qPCR with superior multiplexing capacity

Clinical Chemistry

Volumn 59, Issue 2, 2013, Pages 419-426

  Mokany, Elisa ^a   Tan, Yee Lee ^a   Bone, Simon M ^a   Fuery, Caroline J ^a   Todd, Alison V ^a  

^a SpeeDx Pty Ltd   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; BIOLOGICAL MARKER; DNA; GLUTATHIONE TRANSFERASE P1; K RAS PROTEIN; OLIGONUCLEOTIDE; RETINOIC ACID RECEPTOR BETA; TRANSFERRIN RECEPTOR;

ARTICLE; CALIBRATION; CONTROLLED STUDY; DNA DETERMINATION; DNA METHYLATION; EPIGENETICS; GENE AMPLIFICATION; HUMAN; LIMIT OF DETECTION; MNAZYME QUANTITATIVE POLYMERASE CHAIN REACTION; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROSTATE CANCER; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

DNA PROBES; ENZYMES; GENOME, HUMAN; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; RNA PROBES; SENSITIVITY AND SPECIFICITY;

EID: 84873165888     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2012.192930     Document Type: Article

References (9)

1. Mokany E, Bone SM, Young PE, Doan TB, Todd AV. MNAzymes, a versatile new class of nucleic acid enzymes that can function as biosensors and molecular switches. J Am Chem Soc 2010;132:1051-9.
2. Santoro S, Joyce G. A general purpose RNA cleaving DNA enzyme. Proc Natl Acad Sci U S A 1997; 94:4262-6.
3. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000;25:169 -93.
4. Vener T, Derecho C, Baden J, Wang H, Rajpurohit Y, Skelton J, et al. Development of a multiplexed urine assay for prostate cancer diagnosis. Clin Chem 2008;54:874-82.
5. Kirchner S, Krämer KM, Schulze M, Pauly D, Jacob D, Gessler F, et al. Pentaplexed quantitative realtime PCR assay for the simultaneous detection and quantification of botulinum neurotoxin-producing clostridia in food and clinical samples. Appl Environ Microbiol 2010;76:4387-95.
6. Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, et al. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res 1989;17:2503-16.
7. Ward R, Hawkins N, O'Grady R, Sheehan C, O'Connor T, Impey H, et al. Restriction endonuclease-mediated selective polymerase chain reaction: a novel assay for the detection of K-ras mutations in clinical samples. Am J Pathol 1998;153:373-9.
8. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 1996;93:9821-6.
9. Mitsuyasu RT, Merigan TC, Carr A, Zack JA, Winters MA, Workman C, et al. Phase 2 gene therapy trial of an anti-HIV ribozyme in autologous CD34+ cells. Nat Med 2009;15:285-92