Long term chronic Pseudomonas aeruginosa airway infection in mice

Journal of Visualized Experiments

Volumn , Issue 85, 2014, Pages

  Facchini, Marcella ^a,b   De Fino, Ida ^a,b   Riva, Camilla ^a,b   Bragonzi, Alessandra ^a  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b Italian Cystic Fibrosis Research Foundation   (Italy)

Author keywords

Cystic Fibrosis; Infection; Inflammation; Issue 85; Lung Diseases; Opportunistic Infections; Pseudomonas aeruginosa; Respiratory Tract Infections

Indexed keywords

EID: 84907512786     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51019     Document Type: Article

References (16)

1. Gibson, R., Burns, J.L., Ramsey, B.W. Pathophysiology and management of pulmonary infections in cystic fibrosis. Am. J. Respir. Crit. Care Med. 168, 918-951 (2003).
2. Bragonzi, A. Murine models of acute and chronic lung infection with cystic fibrosis pathogens. IJMM. 300, 584-593 (2010).
3. Cash, H.A., McCullough, B., Johanson, W.G. Jr., Bass, J.A. A rat model of chronic respiratory infection with Pseudomonas aeruginosa. Am. Rev. Respir. Dis. 119, 453-459 (1979).
4. Starke, J.R., Langston, C., Baker, C.J. A mouse model of chronic pulmonary infection with Pseudomonas aeruginosa and Pseudomonas cepacia. Pediatr. Res. 22, 698-702 (1987).
5. Pedersen, S.S., Hansen, B.L., Hansen, G.N. Induction of experimental chronic Pseudomonas aeruginosa lung infection with P. aeruginosa entrapped in alginate microspheres. APMIS. 98, 203-211 (1990).
6. Bragonzi, A. et al. Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model. J. Infect. Dis.192, 410-419 (2005).
7. van Heeckeren, A.M. Murine models of chronic Pseudomonas aeruginosa lung infection. Lab. Anim. 36, 291-312 (2002).
8. Bragonzi, A. et al. Pseudomonas aeruginosa microevolution during cystic fibrosis lung infection establishes clones with adapted virulence AJRCCM. 180, 138-145 (2009).
9. Kukavica-Ibrulj, I., Facchini M, Cigana C, Levesque RC, Bragonzi A. Assessing Pseudomonas aeruginosa virulence and the host response using murine models of acute and chronic lung infection. In: Filloux A, Ramos JL, editors. Methods in Pseudomonas aeruginosa: Humana Press. (2014).
10. Moalli, F. et al. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa. J. Immunol. 186, 5425-534 (2011).
11. Paroni, M. Response of CFTR-deficient mice to long-term Pseudomonas aeruginosa chronic infection and PTX3 therapeutic treatment. J. Infect. Dis. In press (2012).
12. Maxeiner, J., Karwot, R., Hausding, M., Sauer, K.A., Scholtes, P., Finotto, S. A method to enable the investigation of murine bronchial immune cells, their cytokines and mediators. Nat. Protoc. 2, 105-112 (2007).
13. Bragonzi, A. et al. Pseudomonas aeruginosa microevolution during cystic fibrosis lung infection establishes clones with adapted virulence. AJRCCM. In press (2009).
14. Pirone, L. et al. Burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains. Environ. Microbiol. 10, 2773-2784 (2008).
15. Bragonzi, A. et al. Modelling co-infection of the cystic fibrosis lung by Pseudomonas aeruginosa and Burkholderia cenocepacia reveals influences on biofilm formation and host response. PLoS One. 7, e52330 (2012).
16. Bianconi, I. et al. Positive signature-tagged mutagenesis in Pseudomonas aeruginosa: tracking patho-adaptive mutations promoting long-term airways chronic infection. PLoS Pathog. 7, e1001270 (2011).