Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo

PLoS ONE

Volumn 9, Issue 9, 2014, Pages

  Dana, Hod ^a   Chen, Tsai Wen ^a   Hu, Amy ^a   Shields, Brenda C ^a   Guo, Caiying ^a   Looger, Loren L ^a   Kim, Douglas S ^a   Svoboda, Karel ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NEURONAL CALCIUM SENSOR; PARVOVIRUS VECTOR; PROTEIN GCAMP6F; PROTEIN GCAMP6S; UNCLASSIFIED DRUG; CALCIUM;

ADULT; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL POPULATION; CONFOCAL MICROSCOPY; DENTATE GYRUS; FUNCTIONAL NEUROIMAGING; GENOTYPE; HIPPOCAMPAL CA1 REGION; HIPPOCAMPAL CA3 REGION; IN VIVO STUDY; MOLECULAR IMAGING; MOUSE; NERVE CELL; NONHUMAN; PRIMARY MOTOR CORTEX; PRIMARY SOMATOSENSORY CORTEX; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; STRIATE CORTEX; THY1 PROMOTER GENE; TRANSGENIC MOUSE; ANIMAL; ANIMAL BEHAVIOR; CYTOLOGY; METABOLISM; PHYSIOLOGY; VISUAL CORTEX;

MUS MUSCULUS;

ANIMALS; BEHAVIOR, ANIMAL; CALCIUM; MICE; MICE, TRANSGENIC; NEURONS; VISUAL CORTEX;

EID: 84907459289     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0108697     Document Type: Article

References (38)

1. Huber D, Gutnisky D, Peron S, O'Connor D, Wiegert J, et al. (2012) Multiple dynamic representations in the motor cortex during sensorimotor learning. Nature 484: 473-478.
2. Ahrens MB, Li JM, Orger MB, Robson DN, Schier AF, et al. (2012) Brain-wide neuronal dynamics during motor adaptation in zebrafish. Nature 485: 471-477.
3. Stosiek C, Garaschuk O, Holthoff K, Konnerth A (2003) In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci USA 100: 7319-7324.
4. Tian L, Hires SA, Mao T, Huber D, Chiappe ME, et al. (2009) Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nature Methods 6: 875-881.
5. Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, et al. (2010) Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano. Nature methods 7: 729-732.
6. Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS, et al. (2012) Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging. The Journal of Neuroscience 32: 13819-13840.
7. Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, et al. (2013) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499: 295-300.
8. Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, et al. (2014) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nature methods 11: 175-182.
9. Margolis DJ, Lu tcke H, Schulz K, Haiss F, Weber B, et al. (2012) Reorganization of cortical population activity imaged throughout long-term sensory deprivation. Nature Neuroscience 15: 1539-1546.
10. Wallace DJ, Zum Alten Borgloh SM, Astori S, Yang Y, Bausen M, et al. (2008) Single-spike detection in vitro and in vivo with a genetic Ca(2+) sensor. Nature Methods 5: 797-804.
11. Peters AJ, Chen SX, Komiyama T (2014) Emergence of reproducible spatiotemporal activity during motor learning. Nature 510: 263-267.
12. Zariwala HA, Borghuis BG, Hoogland TM, Madisen L, Tian L, et al. (2012) A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. The Journal of Neuroscience 32: 3131-3141.
13. Heim N, Garaschuk O, Friedrich MW, Mank M, Milos RI, et al. (2007) Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor. Nature Methods 4: 127-129.
14. Direnberger S, Mues M, Micale V, Wotjak CT, Dietzel S, et al. (2012) Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. Nature Communications 3: 1031.
15. Hasan MT, Friedrich RW, Euler T, Larkum ME, Giese G, et al. (2004) Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control. PLoS Biology 2: e163.
16. Dez-Garca J, Matsushita S, Mutoh H, Nakai J, Ohkura M, et al. (2005) Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator protein. European Journal of Neuroscience 22: 627-635.
17. Tallini YN, Ohkura M, Choi B-R, Ji G, Imoto K, et al. (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proceedings of the National Academy of Sciences of the USA 103: 4753-4758.
18. Tallini YN, Brekke JF, Shui B, Doran R, Hwang S-m, et al. (2007) Propagated Endothelial Ca2+ Waves and Arteriolar Dilation In Vivo Measurements in Cx40BAC-GCaMP2 Transgenic Mice. Circulation Research 101: 1300-1309.
19. Atkin SD, Patel S, Kocharyan A, Holtzclaw LA, Weerth SH, et al. (2009) Transgenic mice expressing a cameleon fluorescent Ca2+ indicator in astrocytes and Schwann cells allow study of glial cell Ca2+ signals in situ and in vivo. Journal of Neuroscience Methods 181: 212-226.
20. Chen Q, Cichon J, Wang W, Qiu L, Lee S-JR, et al. (2012) Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice. Neuron 76: 297-308.
21. Caroni P (1997) Overexpression of growth-associated proteins in the neurons of adult transgenic mice. Journal of Neuroscience Methods 71: 3-9.
22. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, et al. (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28: 41-51.
23. Behringer R, Gertsenstein M, Vintersten Nagy K, Nagy A (2013) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.
24. Donello JE, Loeb JE, Hope TJ (1998) Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. Journal of Virology 72: 5085-5092.
25. Loeb JE, Cordier WS, Harris ME, Weitzman MD, Hope TJ (1999) Enhanced expression of transgenes from adeno-associated virus vectors with the woodchuck hepatitis virus posttranscriptional regulatory element: implications for gene therapy. Human Gene Therapy 10: 2295-2305.
26. Dong HW (2008) The Allen reference atlas: A digital color brain atlas of the C57Bl/6J male mouse. Hoboken, NJ, USA: John Wiley & Sons Inc.
27. Niell CM, Stryker MP (2008) Highly selective receptive fields in mouse visual cortex. J Neurosci 28: 7520-7536.
28. Pologruto TA, Sabatini BL, Svoboda K (2003) ScanImage: flexible software for operating laser scanning microscopes. Biomed Eng Online 2: 13.
29. Brainard DH (1997) The Psychophysics Toolbox. Spatial Vision 10: 433-436.
30. Pelli DG (1997) The VideoToolbox software for visual psychophysics: transforming numbers into movies. Spatial Vision 10: 437-442.
31. Kerlin AM, Andermann ML, Berezovskii VK, Reid RC (2010) Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex. Neuron 67: 858-871.
32. Ohki K, Chung S, Ch'ng YH, Kara P, Reid RC (2005) Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433: 597-603.
33. Komiyama T, Sato TR, O'Connor DH, Zhang YX, Huber D, et al. (2010) Learning-related fine-scale specificity imaged in motor cortex circuits of behaving mice. Nature 464: 1182-1186.
34. Guo ZV, Li N, Huber D, Ophir E, Gutnisky DA, et al. (2014) Flow of cortical activity underlying a tactile decision in mice. Neuron 81: 179-194.
35. Helmchen F, Imoto K, Sakmann B (1996) Ca2+ buffering and action potentialevoked Ca2+ signaling in dendrites of pyramidal neurons. Biophys J 70: 1069-1081.
36. Hires SA, Tian L, Looger LL (2008) Reporting neural activity with genetically encoded calcium indicators. Brain Cell Biol 36: 69-86.
37. Guo ZV, Hires SA, Li N, O'Connor DH, Komiyama T, et al. (2014) Procedures for behavioral experiments in head-fixed mice. PloS ONE 9: e88678.
38. Xu NL, Harnett MT, Williams SR, Huber D, O'Connor DH, et al. (2012) Nonlinear dendritic integration of sensory and motor input during an active sensing task. Nature 492: 247-251.