Optimization of a whole blood phenotyping assay for enumeration of peripheral blood leukocyte populations in multicenter clinical trials

Journal of Immunological Methods

Volumn 411, Issue , 2014, Pages 23-36

  Hensley McBain, Tiffany ^a   Heit, Antje ^a   De Rosa, Stephen C ^a,b   McElrath, M Juliana ^a,b   Andersen Nissen, Erica ^a,c  

^a Fred Hutchinson Cancer Research Center   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c Cape Town HVTN Immunology Laboratory Hutchinson Center Research Institute of South Africa   (South Africa)

Author keywords

Assay optimization; Clinical trial; Experimental medicine trial; Multiparameter flow cytometry; Phenotyping; Trucount

Indexed keywords

HUMAN IMMUNODEFICIENCY VIRUS VACCINE; IMMUNOLOGICAL ADJUVANT;

ADULT; ARTICLE; BLOOD CELL COUNT; CELL ACTIVATION; CELL ASSAY; CELL COUNTING; CELL MIGRATION; CELL POPULATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DATA ANALYSIS SOFTWARE; DENDRITIC CELL; FLOW CYTOMETRY; HUMAN; IMMUNOPHENOTYPING; MONOCYTE; MULTICENTER STUDY (TOPIC); MYELOID DENDRITIC CELL; NATURAL KILLER CELL; PLASMACYTOID DENDRITIC CELL; PROCESS OPTIMIZATION; TRUCOUNT PANEL 1 ASSAY; ASSAY OPTIMIZATION; BLOOD STORAGE; CLINICAL TRIAL; CRYOPRESERVATION; CYTOLOGY; EXPERIMENTAL MEDICINE TRIAL; FEMALE; IMMUNOLOGY; LEUKOCYTE; MALE; METHODOLOGY; MULTICENTER STUDY; MULTIPARAMETER FLOW CYTOMETRY; PHENOTYPE; STAINING; STANDARD; TRUCOUNT; VACCINATION;

ASSAY OPTIMIZATION; CLINICAL TRIAL; EXPERIMENTAL MEDICINE TRIAL; MULTIPARAMETER FLOW CYTOMETRY; PHENOTYPING; TRUCOUNT;

ADJUVANTS, IMMUNOLOGIC; AIDS VACCINES; BLOOD CELL COUNT; BLOOD PRESERVATION; CRYOPRESERVATION; FEMALE; HUMANS; LEUKOCYTES; MALE; STAINING AND LABELING; VACCINATION;

EID: 84907053651     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.06.002     Document Type: Article

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