Improved PCR assay for the specific detection and quantitation of Escherichia coli serotype O157 in water

Applied Microbiology and Biotechnology

Volumn 98, Issue 18, 2014, Pages 7869-7877

  Cho, Min Seok ^a,c   Joh, Kiseong ^b   Ahn, Tae Young ^c   Park, Dong Suk ^a  

^a National Institute of Animal Science   (South Korea)

^b Hankuk University of Foreign Studies   (South Korea)

^c Dankook University   (South Korea)

Author keywords

Detection; Drinking water; E. coli serotype O157; Putative regulatory protein; Real time qPCR

Indexed keywords

ERROR DETECTION; GENES; POLYMERASE CHAIN REACTION; POTABLE WATER; PROTEINS;

ENVIRONMENTAL WATER SAMPLES; NUCLEOTIDE SEQUENCES; PATHOGENIC BACTERIUM; RAPID IDENTIFICATION; REAL TIME QPCR; REGULATORY PROTEIN; SENSITIVITY AND SPECIFICITY; SEROTYPES;

ESCHERICHIA COLI;

DRINKING WATER; GENOMIC DNA; REGULATOR PROTEIN;

BIOASSAY; BIOMONITORING; COLIFORM BACTERIUM; DRINKING WATER; HOST-PATHOGEN INTERACTION; POLYMERASE CHAIN REACTION; PROTEIN;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIUM DETECTION; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; ENTEROBACTER AEROGENES; ESCHERICHIA COLI O157; LEGIONELLA PNEUMOPHILA; LIMIT OF DETECTION; LIMIT OF QUANTITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SEROTYPE; SHIGELLA BOYDII; SHIGELLA DYSENTERIAE; SHIGELLA FLEXNERI; SHIGELLA SONNEI; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PROCEDURES;

ESCHERICHIA COLI;

BACTERIOLOGICAL TECHNIQUES; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; WATER MICROBIOLOGY;

EID: 84906946237     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5855-8     Document Type: Article

References (32)

1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) Molecular biology of the cell, 4th edn. Garland Science, New York
2. Albuquerque P, Mendes MV, Santos CL, Moradas-Ferreira P, Tavares F (2009) DNA signature-based approaches for bacterial detection and identification. Sci Total Environ 407:3641-3651
3. Atlas RM (2004) Handbook of microbiological media, 3rd edn. CRC Press.
4. Bhagwat AA (2003) Simultaneous detection of Escherichia coli O157: H7, Listeria monocytogenes and Salmonella strains by real-time PCR. Int J Food Microbiol 84:217-224
5. Bielaszewska M, Mellmann A, Zhang W, Köck R, Fruth A, Bauwens A, Peters G, Karch H (2011) Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect Dis 11: 671-676
6. Bischoff C, Luthy J, Altwegg M, Baggi F (2005) Rapid detection of diarrheagenic E. coli by real-time PCR. J Microbiol Methods 61: 335-341 (Pubitemid 40568584)
7. Bloch S, Felczykowska A, nejman-Falen'czyk B (2012) Escherichia coli O104:H4 outbreak - have we learnt a lesson from it? Acta Biochim Pol 59:483-488
8. Bonnet R, Souweine B, Gauthier G, Rich C, Livrelli V, Sirot J, Joly B, Forestier C (1998) Non-O157:H7 Stx2-producing Escherichia coli strains associated with sporadic cases of hemolytic-uremic syndrome in adults. J Clin Microbiol 36:1777-1780 (Pubitemid 28226073)
9. CDC (2001) University outbreak of calicivirus infection mistakenly attributed to Shiga toxin-producing Escherichia coli O157: H7-Virginia, 2000. MMWR Morb Mortal Wkly Rep 50:489-491
10. CDC (2006) Importance of culture confirmation of Shiga toxinproducing Escherichia coli infection as illustrated by outbreaks of gastroenteritis - New York and North Carolina, 2005. MMWR Morb Mortal Wkly Rep 55:1042-1045
11. Cebula TA, Payne WL, Feng P (1995) Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol 33:248-250 (Pubitemid 24382441)
12. Chen J, Zhang L, Paoli GC, Shi C, Tu SI, Shi X (2010) A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis. Int J Food Microbiol 137:168-174
13. Cho MS, Kang MJ, Kim C, Seol Y, Hahn JH, Park SC, Hwang DJ, Ahn T, Park DH, Lim CK, Park DS (2011) Sensitive and specific detection of Xanthomonas oryzae pv. oryzae by real-time bio-PCR using pathovarspecific primers based on an rhs family gene. Plant Dis 95:589-594
14. Feng P, Lampel KA (1994) Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype O157:H7. Microbiology 140:2101-2107 (Pubitemid 24259047)
15. Feng PC, Jinneman K, Scheutz F, Monday SR (2011) Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Appl Environ Microbiol 77:6699-6702
16. Jothikumar N, Griffiths MW (2002) Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays. Appl Environ Microbiol 68:3169-3179
17. Kim HJ, Park SH, Lee TH, Nahm BH, Chung YH, Seo KH, Kim HY (2006) Identification of Salmonella enterica serovar Typhimurium using specific PCR primers obtained by comparative genomics in Salmonella serovars. J Food Prot 69:1653-1661 (Pubitemid 44097970)
18. Lang JM, Hamilton JP, Diaz MGQ, Van Sluys MA, Burgos MRG, Vera Cruz CM, Buell CR, Tisserat NA, Leach JE (2010) Genomics-based diagnostic marker development for Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Plant Dis 94:311-319
19. Lin CK, Lin JC (2007) Development of PCR primers based on a fragment from randomly amplified polymorphic DNA for the detection of Escherichia coli O157:H7/NM. Mol Cell Probes 21:182-189
20. Mukhopadhyay UM, Mukhopadhyay A (2007) Novel multiplex PCR approaches for the simultaneous detection of human pathogens: Escherichia coli O157:H7 and Listeria monocytogenes . J Microbiol Meth 68:193-200
21. Müller D, Greune L, Heusipp G, Karch H, Fruth A, Tschäpe H, Schmidt MA (2007) Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR. Appl Environ Microbiol 73:3380-3390 (Pubitemid 46834990)
22. Muniesa M, Blanco JE, De Simón M, Serra-Moreno R, Blanch AR, Jofre J (2004) Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology 150:2959-2971 (Pubitemid 39317797)
23. h7 real-time PCR assay with an H7 latex agglutination test for confirmation of the H type of Escherichia coli O157:H7. J Food Prot 72:2195-2197
24. Nielsen EM, Andersen MT (2003) Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5′ nuclease PCR assay. J Clin Microbiol 41:2884-2893 (Pubitemid 36842275)
25. Paton AW, Paton JC, Goldwater PN, Manning PA (1993) Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures using the polymerase chain reaction. J Clin Microbiol 31:3063-3067 (Pubitemid 23318410)
26. Paton AW, Paton JC (1998) Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J Clin Microbiol 36:598-602 (Pubitemid 28060342)
27. Paton AW, Paton JC (2002) Direct detection and characterization of shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J Clin Microbiol 40:271-274 (Pubitemid 34027638)
28. Perelle S, Dilasser F, Grout J, Fach P (2004) Detection by 5′-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical cases. Mol Cell Probes 18:185-192 (Pubitemid 38591610)
29. Segerman B, De Medici D, Ehling Schulz M, Fach P, Fenicia L, Fricker M, Wielinga P, Van Rotterdam B, Knutsson R (2011) Bioinformatic tools for using whole genome sequencing as a rapid high resolution diagnostic typing tool when tracing bioterror organisms in the food and feed chain. Int J Food Microbiol 145:S167-S176
30. Tiba MR, Cd M, Carazzolle MF, Leite Dda S (2011) Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFLP and DNA sequencing methods. Braz J Infect Dis 15:144-150
31. Whelan JA, Russel NB, Whelan MA (2003) A method for the absolute quantification of cDNA using real time PCR. J Immunol Methods 278:261-269 (Pubitemid 37281208)
32. Yuan Y, Xu W, Zhai Z, Shi H, Luo Y, Chen Z, Huang K (2009) Universal primer-multiplex PCR approach for simultaneous detection of Escherichia coli, Listeria monocytogenes, and Salmonella spp. in food samples. J Food Sci 74:446-452