Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods

Brazilian Journal of Infectious Diseases

Volumn 15, Issue 2, 2011, Pages 144-150

  Tiba, Monique Ribeiro ^a   de Moura, Claúdia ^a   Carazzolle, Marcelo Falsarella ^a   da Silva Leite, Domingos ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Antigens; Bacterial; Escherichia coli; Polymerase chain reaction; Polymorphism; Restriction fragment length

Indexed keywords

BACTERIAL ANTIGEN; FLAGELLIN; H ANTIGEN; O ANTIGEN; RABBIT ANTISERUM; RESTRICTION ENDONUCLEASE; RESTRICTION ENDONUCLEASE HHAI; RESTRICTION ENDONUCLEASE RSAI; UNCLASSIFIED DRUG;

AGGLUTINATION TEST; AMINO ACID SEQUENCE; AMPLICON; ANTIGEN EXPRESSION; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEROTYPING;

EID: 79959577583     PISSN: 14138670     EISSN: 16784391     Source Type: Journal    
DOI: 10.1590/S1413-86702011000200009     Document Type: Article

References (12)

1. Machado J, Grimont F, Grimont PAD. Identification of Escherichia coli fagellar types by restriction of the amplifed fiC gene. Res Microbiol 2000; 151:535-546.
2. Prager R, Strutz U, Fruth A, Tschäpe H. Subtyping of pathogenic Escherichia coli strains using fagellar (H) - antigens: serotyping versus fiC polymorphisms. Int J Med Microbiol 2003; 292:477-486.
3. Wang L, Rothemund D, Curd H, Reeves PR. Species-wide variation in the Escherichia coli fagellin (H-antigen) gene. J Bacteriol 2003; 185:2936-2943.
4. Ørskov I, Ørskov F. Escherichia coli serotyping and in man and animals. Can J Microbiol 1992; 38:699-704.
5. Ratiner YA. Temperature-dependent fagellar antigen phase variation in Escherichia coli. Res Microbiol 1999; 150:457-463.
6. Schoenhals G, Whitfeld C. Comparative analysis of fagellin sequences from Escherichia coli strains possessing serologi-cally distinct fagellar flaments with a shared complex surface pattern. J Bacteriol 1993; 175:5395-5402.
7. Fields PI, Blom K, Hughes HJ, Helsel LO, Feng P, Swaminathan B. Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-Restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM. J Clin Microbiol 1997; 35:1066-1070.
8. Amhaz JMK, Andrade A, Bando SY, Tanaka TL, Moreira-Filha CA, Martinez MB. Molecular typing and phylogenetic analysis of enteroinvasive Escherichia coli using the fiC gene sequence. FEMS Microbiol Lett 2004; 235:259-264.
9. Scheutz F, Cheasty T,Woodward D, Smith HR. Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181. AP-MIS 2004; 112:569-584.
10. Ørskov F, Ørskov I, Jann B, Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev 1977; 41:667-710.
11. Ewing WH, Edwards, PR. The genus Escherichia. Identification of Enterobacteriaceae, Burgess, Minneapolis, 1983.
12. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. New York CSHL, 1989.