Comparison of peptide nucleic acid fluorescence in situ hybridization assays with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the identification of bacteria and yeasts from blood cultures and cerebrospinal fluid cultures

Clinical Microbiology and Infection

Volumn 20, Issue 8, 2014, Pages

  Calderaro, A ^a   Martinelli, M ^a   Motta, F ^a   Larini, S ^a   Arcangeletti, M C ^a   Medici, M C ^a   Chezzi, C ^a   De Conto, F ^a  

^a UNIVERSITY OF PARMA   (Italy)

Author keywords

Bacteraemia; Blood culture; Cerebrospinal fluid; Fungaemia; Matrix assisted laser desorption ionization time of flight; Peptide nucleic acid fluorescence in situ hybridization

Indexed keywords

PEPTIDE NUCLEIC ACID;

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOOD SAMPLING; CEREBROSPINAL FLUID CULTURE; CHILD; COMPARATIVE STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROCOCCUS FAECALIS; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGAL STRAIN; FUNGUS IDENTIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE COCCI; GRAM STAINING; HUMAN; INFANT; MAJOR CLINICAL STUDY; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROSCOPY; NEWBORN; PRIORITY JOURNAL; PROSPECTIVE STUDY; SPECIES IDENTIFICATION; STAPHYLOCOCCUS AUREUS; YEAST; BACTEREMIA; BACTERIA; BLOOD; CENTRAL NERVOUS SYSTEM FUNGAL INFECTIONS; CEREBROSPINAL FLUID; DIAGNOSTIC TEST; DIAGNOSTIC USE; EVALUATION STUDY; FUNGEMIA; FUNGUS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MENINGITIS, BACTERIAL; MICROBIOLOGY; MIDDLE AGED; PRESCHOOL CHILD; PROCEDURES; TIME; VERY ELDERLY; YOUNG ADULT;

ENTEROCOCCUS; STAPHYLOCOCCUS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTEREMIA; BACTERIA; BLOOD; CENTRAL NERVOUS SYSTEM FUNGAL INFECTIONS; CEREBROSPINAL FLUID; CHILD; CHILD, PRESCHOOL; DIAGNOSTIC TESTS, ROUTINE; FEMALE; FUNGEMIA; FUNGI; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INFANT; INFANT, NEWBORN; MALE; MENINGITIS, BACTERIAL; MIDDLE AGED; PEPTIDE NUCLEIC ACIDS; PROSPECTIVE STUDIES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS; YOUNG ADULT;

EID: 84906790195     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/1469-0691.12490     Document Type: Article

References (30)

1. Shepard JR, Addison RM, Alexander BD et al. Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual color identification of C. albicans and C. glabrata directly from blood culture bottles. J Clin Microbiol 2008; 46: 50-55.
2. Trenholme GM, Kaplan RL, Karakusis PH et al. Clinical impact of rapid identification and susceptibility testing of bacterial blood culture isolates. J Clin Microbiol 1989; 27: 1342-1345.
3. Weinstein MP, Towns ML, Quartey SM et al. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteraemia and fungaemia in adults. Clin Infect Dis 1997; 24: 584-602.
4. Mylotte JM, Tayara A. Blood culture: clinical aspects and controversies. Eur J Clin Microbiol Infect Dis 2000; 19: 157-163.
5. Wilson ML, Mitchell M, Morris AJ et al., eds. Principles and procedures for blood cultures; approved guideline. CLSI document M47-A. Wayne, PA: Clinical and Laboratory Standards Institute, 2007.
6. Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, Aepfelbacher M. Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption - ionization time of flight mass spectrometry fingerprinting. J Clin Microbiol 2010; 48: 1584-1591.
7. Harris DM, Hata DJ. Rapid identification of bacteria and Candida, using PNA FISH from blood and peritoneal fluid cultures: a retrospective clinical study. Ann Clin Microbiol Antimicrob 2013; 12: 1-9.
8. Peters RPH, Savelkoul PHM, Simoons-Smit AM, Danner SA, Vandenbroucke-Grauls CMJE, van Agtmael MA. Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol 2006; 44: 119-123.
9. Spanu T, Posteraro B, Fiori B et al. Direct MALDI-TOF mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infection: an observational study in two large microbiology laboratories. J Clin Microbiol 2012; 50: 176-179.
10. Wellinghausen N, Wirths B, Franz AR, Karolyi L, Marre R, Reischl U. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn Microbiol Infect Dis 2004; 48: 229-241.
11. Forrest GN, Roghmann MC, Toombs LS et al. Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteraemia: delivering earlier effective antimicrobial therapy. Antimicrob Agents Chemother 2008; 52: 3558-3563.
12. Sogaard M, Hansen DS, Fiandaca MJ, Stender H, Schonheyder HC. Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures. J Med Microbiol 2007; 56: 914-917.
13. Calderaro A, Piccolo G, Montecchini S et al. MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension. J Proteomics 2013; 78: 273-280.
14. Cherkaoui A, Hibbs J, Emonet S et al. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to species level. J Clin Microbiol 2010; 48: 1169-1175.
15. Drancourt M. Detection of microorganisms in blood specimens using matrix-assisted laser desorption/ionization - time of flight mass spectrometry: a review. Clin Microbiol Infect 2010; 16: 1620-1625.
16. Seng P, Drancourt M, Gourlet F et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption/ionization - time of flight mass spectrometry. Clin Infect Dis 2009; 49: 543-551.
17. van Veen SQ, Claas EC, Kuijper EJ. High throughput identification of bacteria and yeasts by matrix-assisted laser desorption/ionization - time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol 2010; 48: 900-907.
18. Forrest GN, Mehta S, Weekes E, Lincalis DP, Johnson JK, Venezia RA. Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother 2006; 58: 154-158.
19. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadeguate antimicrobial treatment of blood stream infections on patient outcomes in the ICU setting. Chest 2000; 118: 146-155.
20. Morgan M, Marlowe E, Della-Latta P et al. Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol 2010; 48: 2268-2270.
21. Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol 2007; 45: 3802-3803.
22. Rigby S, Procop GW, Haase G et al. Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles. J Clin Microbiol 2002; 40: 2182-2186.
23. Baron EJ, Weinstein MP, Dunne WM, Yagupsky P, Welch DF, Wilson DM. Cumitech 1C: blood Cultures IV. Coordinating editor Baron EJ. Washington D.C.: ASM Press, 2005.
24. Martiny D, Busson L, Wybo I, El Haj RA, Dediste A, Vandenberg O. Comparison of the Microflex LT and Vitek MS systems for routine identification of bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012; 50: 1313-1325.
25. Kärpänoja P, Harju I, Rantakokko-Jalava K, Haanperä M, Sarkkinen H. Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of viridians group streptococci. Eur J Clin Microbiol Infect Dis 2013, doi: 10.1007/s10096-013-2012-8. [Epub ahead of print].
26. Deck MK, Anderson ES, Buckner RJ et al. Multicenter evaluation of the Staphylococcus QuickFISH method for simultaneous identification of Staphylococcus aureus and coagulase-negative staphylococci directly from blood culture bottles in less than 30 minutes. J Clin Microbiol 2012; 50: 1994-1998.
27. Akcam FZ, Yayli G, Uskun E, Kaya O, Demir C. Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids. Res Microbiol 2006; 57: 433-436.
28. Lagacé-Wiens PRS, Adam HJ, Karlowsky JA et al. Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization - time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time. J Clin Microbiol 2012; 50: 3324-3328.
29. Leli C, Cenci E, Cardaccia A et al. Rapid identification of bacterial and fungal pathogens from positive blood cultures by MALDI-TOF MS. Int J Med Microbiol 2013; 303: 205-209.
30. Schubert S, Weinert K, Wagner C et al. Novel, improved sample preparation for rapid, direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. J Mol Diagn 2011; 13: 701-706.