Rapid identification of bacteria and candida using pna-fish from blood and peritoneal fluid cultures: A retrospective clinical study

Annals of Clinical Microbiology and Antimicrobials

Volumn 12, Issue 1, 2013, Pages

  Harris, Dana M ^a   Hata, D Jane ^a  

^a MAYO CLINIC   (United States)

Author keywords

Bacteremia; Blood culture; Fungemia; Peritoneal fluid; PNA FISH

Indexed keywords

CASPOFUNGIN; FLUCONAZOLE; VANCOMYCIN;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BLOOD CULTURE; CANDIDA; CANDIDIASIS; COAGULASE NEGATIVE STAPHYLOCOCCUS; COMPARATIVE STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HEALTH CARE COST; HUMAN; MAJOR CLINICAL STUDY; MALE; MEDICAL RECORD REVIEW; NONHUMAN; PEPTIDE NUCLEIC ACID FLUORESCENT IN SITU HYBRIDIZATION; PERITONEAL FLUID; RETROSPECTIVE STUDY;

ANTI-INFECTIVE AGENTS; ASCITIC FLUID; BACTEREMIA; BLOOD; CANDIDA; GRAM-POSITIVE BACTERIA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PEPTIDE NUCLEIC ACIDS; RETROSPECTIVE STUDIES; STAPHYLOCOCCUS; TIME FACTORS; VANCOMYCIN;

EID: 84871871068     PISSN: None     EISSN: 14760711     Source Type: Journal    
DOI: 10.1186/1476-0711-12-2     Document Type: Article

References (21)

1. Heron M, Hoyert DL, Murphy SL, Xu J, Kochanek KD, Tejada-Vera B. Deaths: final data for 2006. Natl Vital Stat Rep 2009, 57(14):1-134.
2. Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Inf Dis 2004, 39(3):309-317.
3. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest 2000, 118(1):146-155. 10.1378/chest.118.1.146, 10893372.
4. Baron EJ, Weinstein MP, Dunne WMJ, Yagupsky P, Welch DF, Wilson DM. Cumitech 1C: Blood Cultures IV 2005, Washington D.C: ASM Press, 1.
5. Klouche M, Schroder U. Rapid methods for diagnosis of bloodstream infections. Clin Chem Lab Med 2008, 46(7):888-908.
6. Forrest GN, Roghmann MC, Toombs LS, Johnson JK, Weekes E, Lincalis DP, Venezia RA. Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: delivering earlier effective antimicrobial therapy. Antimicrob Agents Chemother 2008, 52(10):3558-3563. 10.1128/AAC.00283-08, 2565911, 18663022.
7. Sogaard M, Hansen DS, Fiandaca MJ, Stender H, Schonheyder HC. Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures. J Med Microbiol 2007, 56(Pt 7):914-917.
8. Forrest GN, Mehta S, Weekes E, Lincalis DP, Johnson JK, Venezia RA. Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother 2006, 58(1):154-158. 10.1093/jac/dkl146, 16636084.
9. Morgan M, Marlowe E, Della-Latta P, Salimnia H, Novak-Weekley S, Wu F, Crystal BS. Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures. J Clin Microbiol 2010, 48(6):2268-2270. 10.1128/JCM.00166-10, 2884511, 20357212.
10. Reller ME, Mallonee AB, Kwiatkowski NP, Merz WG. Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. J Clin Microbiol 2007, 45(11):3802-3803. 10.1128/JCM.01127-07, 2168530, 17804657.
11. Rigby S, Procop GW, Haase G, Wilson D, Hall G, Kurtzman C, Oliveira K, Von Oy S, Hyldig-Nielsen JJ, Coull J, et al. Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles. J Clin Microbiol 2002, 40(6):2182-2186. 10.1128/JCM.40.6.2182-2186.2002, 130801, 12037084.
12. Shepard JR, Addison RM, Alexander BD, Della-Latta P, Gherna M, Haase G, Hall G, Johnson JK, Merz WG, Peltroche-Llacsahuanga H, et al. Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles. J Clin Microbiol 2008, 46(1):50-55. 10.1128/JCM.01385-07, 2224311, 17977998.
13. GraphPad Software QuickCalcs http://graphpad.com/quickcalcs.
14. Book R. Pharmacy Fundamental Reference 2009, Montvale, New Jersey: Physicians Desk Reference Inc, 113.
15. Hall L, Le Febre KM, Deml SM, Wohlfiel SL, Wengenack NL. Evaluation of the Yeast Traffic Light PNA FISH probes for identification of Candida species from positive blood cultures. J Clin Microbiol 2012, 50(4):1446-1448. 10.1128/JCM.06148-11, 3318520, 22238445.
16. Akcam FZ, Yayli G, Uskun E, Kaya O, Demir C. Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids. Res Microbiol 2006, 157(5):433-436. 10.1016/j.resmic.2005.10.005, 16364602.
17. Fuller DD, Davis TE. Comparison of BACTEC plus Aerobic/F, Anaerobic/F, Peds Plus/F, and Lytic/F media with and without fastidious organism supplement to conventional methods for culture of sterile body fluids. Diagn Microbiol Infect Dis 1997, 29(4):219-225. 10.1016/S0732-8893(97)00164-8, 9458978.
18. Sorlin P, Mansoor I, Dagyaran C, Struelens MJ. Comparison of resin-containing BACTEC Plus Aerobic/F* medium with conventional methods for culture of normally sterile body fluids. J Med Microbiol 2000, 49(9):787-791.
19. Pfaller MA, Diekema DJ. Role of sentinel surveillance of candidemia: trends in species distribution and antifungal susceptibility. J Clin Microbiol 2002, 40(10):3551-3557. 10.1128/JCM.40.10.3551-3557.2002, 130894, 12354845.
20. Collins CD, Eschenauer GA, Salo SL, Newton DW. To test or not to test: a cost minimization analysis of susceptibility testing for patients with documented Candida glabrata fungemias. J Clin Microbiol 2007, 45(6):1884-1888. 10.1128/JCM.00192-07, 1933067, 17409208.
21. Hermsen ED, Shull SS, Klepser DG, Iwen PC, Armbrust A, Garrett J, Freifeld AG, Rupp ME. Pharmacoeconomic analysis of microbiologic techniques for differentiating staphylococci directly from blood culture bottles. J Clin Microbiol 2008, 46(9):2924-2929. 10.1128/JCM.00623-08, 2546718, 18614649.