A simple in vitro acylation assay based on optimized HlyA and HlyC purification

Analytical Biochemistry

Volumn 464, Issue , 2014, Pages 17-23

  Thomas, Sabrina ^a   Smits, Sander H J ^a   Schmitt, Lutz ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Erythrocytes; Hemolytic activity; Ion binding; RTX toxin; UPEC

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; PROTEINS; PURIFICATION; TOXIC MATERIALS;

ERYTHROCYTES; HEMOLYTIC ACTIVITY; ION BINDING; RTX TOXIN; UPEC;

ACYLATION;

ACYLTRANSFERASE; BACTERIAL PROTEIN; ESCHERICHIA COLI ENDOTOXIN; HLYA PROTEIN; HLYC PROTEIN; LYSINE; MUTANT PROTEIN; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; HEMOLYSIN; HLYA PROTEIN, E COLI; HLYC PROTEIN, E COLI; PRIMER DNA;

ACYLATION; ANIMAL CELL; ARTICLE; BACTERIUM MUTANT; ENZYME ACTIVE SITE; ENZYME CONFORMATION; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN FAMILY; PROTEIN PURIFICATION; ISOLATION AND PURIFICATION; NUCLEOTIDE SEQUENCE;

ACYLATION; ACYLTRANSFERASES; BASE SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI PROTEINS; HEMOLYSIN PROTEINS; IN VITRO TECHNIQUES;

EID: 84906776080     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.07.001     Document Type: Article

References (27)

1. I. Gentschev, G. Dietrich, and W. Goebel The E. coli alpha-hemolysin secretion system and its use in vaccine development Trends Microbiol. 10 2002 39 45 (Pubitemid 34075263)
2. I. Linhartova, L. Bumba, J. Masin, M. Basler, R. Osicka, J. Kamanova, K. Prochazkova, I. Adkins, J. Hejnova-Holubova, L. Sadilkova, J. Morova, and P. Sebo RTX proteins: a highly diverse family secreted by a common mechanism FEMS Microbiol. Rev. 34 2010 1076 1112
3. U. Baumann, S. Wu, K.M. Flaherty, and D.B. McKay Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif EMBO J. 12 1993 3357 3364 (Pubitemid 23256422)
4. S. Letoffe, P. Delepelaire, and C. Wandersman Protein secretion in gram-negative bacteria: assembly of the three components of ABC protein-mediated exporters is ordered and promoted by substrate binding EMBO J. 15 1996 5804 5811 (Pubitemid 26375502)
5. T. Thanabalu, E. Koronakis, C. Hughes, and V. Koronakis Substrate-induced assembly of a contiguous channel for protein export from E. coli: reversible bridging of an inner-membrane translocase to an outer membrane exit pore EMBO J. 17 1998 6487 6496 (Pubitemid 28521784)
6. S. Thomas, I.B. Holland, and L. Schmitt The type 1 secretion pathway - the hemolysin system and beyond Biochim. Biophys. Acta 2013 1843 1629 1641
7. P.J. Bakkes, S. Jenewein, S.H. Smits, I.B. Holland, and L. Schmitt The rate of folding dictates substrate secretion by the Escherichia coli hemolysin type 1 secretion system J. Biol. Chem. 285 2010 40573 40580
8. H. Lilie, W. Haehnel, R. Rudolph, and U. Baumann Folding of a synthetic parallel beta-roll protein FEBS Lett. 470 2000 173 177 (Pubitemid 30151091)
9. 2+ in Escherichia coli highlight two putative influx mechanisms in response to changes in extracellular calcium Cell Calcium 25 1999 265 274
10. K.B. Lim, C.R. Walker, L. Guo, S. Pellett, J. Shabanowitz, D.F. Hunt, E.L. Hewlett, A. Ludwig, W. Goebel, R.A. Welch, and M. Hackett Escherichia coli alpha-hemolysin (HlyA) is heterogeneously acylated in vivo with 14-, 15-, and 17-carbon fatty acids J. Biol. Chem. 275 2000 36698 36702 (Pubitemid 32002074)
11. P. Stanley, L.C. Packman, V. Koronakis, and C. Hughes Fatty acylation of two internal lysine residues required for the toxic activity of Escherichia coli hemolysin Science 266 1994 1992 1996
12. J.P. Issartel, V. Koronakis, and C. Hughes Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation Nature 351 1991 759 761 (Pubitemid 21896685)
13. P. Stanley, V. Koronakis, and C. Hughes Acylation of Escherichia coli hemolysin: a unique protein lipidation mechanism underlying toxin function Microbiol. Mol. Biol. Rev. 62 1998 309 333 (Pubitemid 28270277)
14. L.M. Worsham, M.S. Trent, L. Earls, C. Jolly, and M.L. Ernst-Fonberg Insights into the catalytic mechanism of HlyC, the internal protein acyltransferase that activates Escherichia coli hemolysin toxin Biochemistry 40 2001 13607 13616 (Pubitemid 33130788)
15. S.L. Chen, C.S. Hung, J. Xu, C.S. Reigstad, V. Magrini, A. Sabo, D. Blasiar, T. Bieri, R.R. Meyer, P. Ozersky, J.R. Armstrong, R.S. Fulton, J.P. Latreille, J. Spieth, T.M. Hooton, E.R. Mardis, S.J. Hultgren, and J.I. Gordon Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach Proc. Natl. Acad. Sci. U.S.A. 103 2006 5977 5982
16. D.J. Slotboom, R.H. Duurkens, K. Olieman, and G.B. Erkens Static light scattering to characterize membrane proteins in detergent solution Methods 46 2008 73 82
17. K. Rutherford, J. Parkhill, J. Crook, T. Horsnell, P. Rice, M.A. Rajandream, and B. Barrell Artemis: sequence visualization and annotation Bioinformatics 16 2000 944 945
18. C. Hyland, L. Vuillard, C. Hughes, and V. Koronakis Membrane interaction of Escherichia coli hemolysin: flotation and insertion-dependent labeling by phospholipid vesicles J. Bacteriol. 183 2001 5364 5370 (Pubitemid 32802609)
19. A. Ludwig, F. Garcia, S. Bauer, T. Jarchau, R. Benz, J. Hoppe, and W. Goebel Analysis of the in vivo activation of hemolysin (HlyA) from Escherichia coli J. Bacteriol. 178 1996 5422 5430 (Pubitemid 26304375)
20. M. Moayeri, and R.A. Welch Prelytic and lytic conformations of erythrocyte-associated Escherichia coli hemolysin Infect. Immun. 65 1997 2233 2239 (Pubitemid 27237302)
21. L. Sanchez-Magraner, A.L. Cortajarena, F.M. Goni, and H. Ostolaza Membrane insertion of Escherichia coli alpha-hemolysin is independent from membrane lysis J. Biol. Chem. 281 2006 5461 5467 (Pubitemid 43853010)
22. H. Ostolaza, A. Soloaga, and F.M. Goni The binding of divalent cations to Escherichia coli alpha-haemolysin Eur. J. Biochem. 228 1995 39 44
23. J.M. Nicaud, N. Mackman, L. Gray, and I.B. Holland Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001 FEBS Lett. 187 1985 339 344 (Pubitemid 15246286)
24. K.R. Hardie, J.P. Issartel, E. Koronakis, C. Hughes, and V. Koronakis In vitro activation of Escherichia coli prohaemolysin to the mature membrane-targeted toxin requires HlyC and a low molecular-weight cytosolic polypeptide Mol. Microbiol. 5 1991 1669 1679 (Pubitemid 21896196)
25. A. Dobereiner, A. Schmid, A. Ludwig, W. Goebel, and R. Benz The effects of calcium and other polyvalent cations on channel formation by Escherichia coli alpha-hemolysin in red blood cells and lipid bilayer membranes Eur. J. Biochem. 240 1996 454 460 (Pubitemid 26292755)
26. K.G. Langston, L.M. Worsham, L. Earls, and M.L. Ernst-Fonberg Activation of hemolysin toxin: relationship between two internal protein sites of acylation Biochemistry 43 2004 4338 4346 (Pubitemid 38445655)
27. L.M. Worsham, K.G. Langston, and M.L. Ernst-Fonberg Thermodynamics of a protein acylation: activation of Escherichia coli hemolysin toxin Biochemistry 44 2005 1329 1337 (Pubitemid 40209012)