Analysis of the in vivo activation of hemolysin (HlyA) from Escherichia coli

Journal of Bacteriology

Volumn 178, Issue 18, 1996, Pages 5422-5430

  Ludwig, Albrecht ^a   Garcia, Fernando ^a,c   Bauer, Susanne ^a   Jarchau, Thomas ^a,d   Benz, Roland ^a   Hoppe, Jürgen ^b   Goebel, Werner ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c UNIVERSIDAD DE COSTA RICA   (Costa Rica)

^d UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLATION; ARTICLE; BILAYER MEMBRANE; DNA FLANKING REGION; ENDOTHELIUM CELL; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME MODIFICATION; ERYTHROCYTE MEMBRANE; ESCHERICHIA COLI; GENE CONVERSION; LIPID BILAYER; NONHUMAN; OPERON; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0029812094     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.18.5422-5430.1996     Document Type: Article

References (43)

1. Benz, R., A. Schmid, W. Wagner, and W. Goebel. 1989. Pore formation by the Escherichia coli hemolysin: evidence for an association-dissociation equilibrium of the pore-forming aggregates. Infect, Immun. 57:887-895.
2. Bhakdi, S., and E. Martin. 1991. Superoxide generation by human neutrophils induced by low doses of Escherichia coli hemolysin. Infect, Immun. 59:2955-2962.
3. Bhakdi, S., M. Muhly, S. Korom, and G. Schmidt. 1990. Effects of Escherichia coli hemolysin on human monocytes. Cytocidal action and stimulation of interleukin 1 release. J. Clin. Invest. 85:1746-1753.
4. Boehm, D. F., R. A. Welch, and I. S. Snyder. 1990. Domains of Escherichia coli hemolysin (HlyA) involved in binding of calcium and erythrocytc membranes. Infect. Immun. 58:1959-1964.
5. Cavalieri, S. J., G. A. Bohach, and I. S. Snyder. 1984. Escherichia coli α-hemolysin: characteristics and probable role in pathogenicity. Microbiol. Rev. 48:326-343.
6. Chang, Y.-F., R. Young, and D. K. Struck. 1989. Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae. DNA 8:635-647.
7. Coote, J. G. 1992. Structural and functional relationships among the RTX toxin determinants of Gram-negative bacteria. FEMS Microbiol. Rev. 88:137-162.
8. Felmlee, T., S. Pellett, and R. A. Welch. 1985. Nucleotide sequence of an Escherichia coli chromosomal hemolysin. J. Bacteriol. 163:94-105.
9. Frey, J., R. Meier, D. Gygi, and J. Nicolet. 1991. Nuclecitide sequence of the hemolysin I gene from Actinobacillus pleuropneumoniae. Infect. Immun. 59:3025-3032.
10. Gentschev, I, and W. Goebel. 1992. Topological and functional studies on HlyB of Escherichia coli. Mol. Gen. Genet. 232:40-48.
11. Glaser, P., D. Ladant, O. Sezer, F. Pichot, A. Ullmann, and A. Danchin. 1988. The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli. Mol. Microbiol. 2:19-30.
12. Goebel, W., and J. Hedgpeth. 1982. Cloning and functional characterization of the plasmid-encodcd hemolysin determinant of Escherichia coli. J Bacteriol. 151:1290-1298.
13. Grimminger, F., C. Scholz, S. Bhakdi, and W. Seeger. 1991. Subhemolytic doses of Escherichia coli hemolysin evoke large quantities of lipoxygenasc products in human neutrophils. J. Biol. Chem. 266:14262-14269.
14. Hackett, M., L. Guo, J. Shabanowitz, D. F. Hunt, and E. L. Hewlett. 1994. Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis. Science 266:433-435.
15. Hackett, M., C. B. Walker, L. Guo, M. C. Gray, S. Van Cuyk, A. Ullmann, J. Shabanowitz, D. F. Hunt, E. L. Hewlett, and P. Sebo. 1995. Hemolytic, but not cell-invasive activity, of adenylate cyclase toxin is selectively affected by differential fatty-acylation in Escherichia coli. J. Biol. Chem. 270:20250-20253.
16. Hardie, K. R., J.-P. Issartel, E. Koronakis, C. Hughes, and V. Koronakis. 1991. In vitro activation of Escherichia coli prohaemolysin to the mature membrane-targeted toxin requires HlyC and a low molecular-weight cytosolic polypeptide. Mol. Microbiol. 5:1669-1679.
17. Hess, J., W. Wels, M. Vogel, and W. Goebel. 1986. Nucleotidc sequence of a plasmid-encoded hemolysin determinant and its comparison with a corresponding chromosomal hemolysin sequence. FEMS Microbiol. Lett. 34:1-11.
18. Issartel, J.-P., V. Koronakis, and C. Hughes. 1991. Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation. Nature 351:759-761.
19. Jansen, R., J. Briaire, E. M. Kamp, A. L. J. Gielkens, and M. A. Smits. 1993 Cloning and characterization of the Actinobacillus pleuropneumoniae-RTX-toxin III (ApxIII) gene. Infect. Immun. 61:947-954.
20. Jarchau, T., T. Chakraborty, F. Garcia, and W. Goebel. 1994. Selection for transport competence of C-terminal polypeptidcs derived from Escherichia coli hemolysin: the shortest peptide capable of autonomous HlyB/HlyD-dependent secretion comprises the C-terminal 62 amino acids of HlyA. Mol. Gen. Genet. 245:53-60.
21. Jonas, D., B. Schultheis, C. Klas, P. H. Krammer, and S. Bhakdi. 1993. Cytocidal effects of Escherichia coli hemolysin on human T lymphocytes. Infect. Immun. 61:1715-1721.
22. Keane, W. F., R. Welch, G. Gekker, and P. K. Peterson. 1987. Mechanism of Escherichia coli alpha-hemolysin-induced injury to isolated renal tubular cells. Am. J. Pathol. 126:350-357.
23. König, B., W. König, J. Scheffer, J. Hacker, and W. Goebel. 1986. Role of Escherichia coli alpha-hemolysin and bacterial adherence in infection: requirement for release of inflammatory mediators from granulocytes and mast cells. Infect. Immun. 54:886-892.
24. König, B., W. Schönfeld, J. Scheffer, and W. König. 1990. Signal transduction in human platelets and inflammatory mediator release induced by genetically cloned hemolysin-positive and -negative Escherichia coli strains. Infect. Immun. 58:1591-1599.
25. Kraig, E., T. Dailey, and D. Kolodrubetz. 1990. Nucleotide sequence of the leukotoxin gene from Actinobacillus actinomycetemcomitans: homology to the alpha-hcmolysin/leukotoxin gene family. Infect. Immun. 58:920-929.
26. Kramer, W., V. Drutsa, H.-W. Jansen, B. Kramer, M. Pflugfelder, and H.-J. Fritz. 1984. The gapped duplex DMA approach to oligonucleotide-directed mutation construction. Nucleic Acids Res. 12:9441-9456.
27. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bactcriophage T4. Nature 227:680-685.
28. Lo, R. Y. C., C. A. Strathdee, and P. E. Shewen. 1987. Nucleotidc sequence of the leukotoxin genes of Pasteurella haemolytica A1. Infect. Immun. 55:1987-1996.
29. 2+ -dependent binding to crythrocytes. Mol. Gen. Genet. 214:553-561.
30. Ludwig, A., A. Schmid, R. Benz, and W. Goebel. 1991. Mutations affecting pore formation by haemolysin from Escherichia coli. Mol. Gen. Genet. 226:198-208.
31. Ludwig, A., M. Vogel, and W. Goebel. 1987. Mutations affecting activity and transport of haemolysin in Escherichia coli. Mol. Gen. Genet. 206:238-245.
32. O'Farrell, P. H. 1975. High resolution two-dimensional clectrophoresis of proteins. J. Biol. Chem. 250:4007-4021.
33. Oropeza-Wekerle, R.-L., E. Müller, P. Kern, R. Meyermann, and W. Goebel. 1989. Synthesis, inactivation, and localization of extracellular and intracellular Escherichia coli hemolysins. J. Bacteriol. 171:2783-2788.
34. Rock, C. O., and J. L. Garwin. 1979. Preparative enzymatic synthesis and hydrophobic chromatography of acyl-acyl carrier protein. J. Biol. Chem. 254:7123-7128.
35. Rowe, G. E., S. Pellett, and R. A. Welch. 1994. Analysis of toxinogenic functions associated with the RTX repeat region and monoclonal antibody D12 epitope of Escherichia coli hemolysin. Infect. Immun. 62:579-588.
36. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
37. Schülein, R., I. Gentschev, H.-J. Mollenkopf, and W. Goebel. 1992. A topological model for the haemolysin translocator protein HlyD. Mol. Gen. Genet. 234:155-163.
38. Stanley, P., L. C. Packman, V. Koronakis, and C. Hughes. 1994. Fatty acylation of two internal lysine residues required for the toxic activity of Escherichia coli hemolysin. Science 266:1992-1996.
39. Suttorp, N., B. Flöer, H. Schnittler, W. Seeger, and S. Bhakdi. 1990. Effects of Escherichia coli hemolysin on endothelial cell function. Infect. Immun. 58:3796-3801.
40. Vogel, M., J. Hess, I. Then, A. Juarez, and W. Goebel. 1988. Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli. Mol. Gen. Genet. 212:76-84.
41. Wagner, W, M. Vogel, and W. Goebel. 1983. Transport of hemolysin across the outer membrane of Escherichia coli requires two functions. J. Bacteriol. 154:200-210.
42. Wang, R., S. J. Seror, M. Blight, J. M. Pratt, J. K. Broome-Smith, and I. B. Holland. 1991. Analysis of the membrane organization of an Escherichia coli protein translocator, HlyB. a member of a large family of prokaryote and eukaryote surface transport proteins. J. Mol. Biol. 217:441-454.
43. Welch, R. A. 1991. Pore-forming cytolysins of Gram-negative bacteria. Mol. Microbiol. 5:521-528.